Figure 5From: Identification of an N-terminal 27 kDa fragment of Mycoplasma pneumoniae P116 protein as specific immunogen in M. pneumoniaeinfections Comparative ELISA analysis of recombinant proteins with commercial assay with patient sera negative for M. pneumoniae infection. Comparative ELISA analysis of purified recombinant P116(N-27), P1(C-40) and P116(N-27) + P1(C-40) with commercial ELISA for IgM antibodies to M. pneumoniae with 31 negative patient sera. For each serum the "blue square" shows reactivity with the P116(N-27), whereas "purple, yellow and green squares" show reactivity with the P1(C-40), P116(N-27) + P1(C-40) and commercial ELISA kit respectively (Inset shows western blot results for the three patients negative by commercial ELISA, but positive in P116(N-27), or P1(C-40) or P116(N-27)+ P1(C-40) ELISAs as well as in western blot with P116(N-27)).Back to article page