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Table 1 Mean concentration of Brucella melitensis DNA obtained with five purification kits from tissue samples of two dromedaries.

From: Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

  QIAGEN Peqlab Macherey-Nagel MoBio Roche
fg/μl mean SD mean SD mean SD mean SD mean SD
D1452/06           
Liver 8915 15150 186 17 76 9 neg   207 26
Spleen 10 2 5 2 101 21 neg*   402 20
Lung 225 66 72 11 216 55 71 23 2252 89
Right kidney 1377 19 73 27 315 24 101 19 757 53
Prescapular Ln 415 65 111 13 41 11 123 20 1338 355
Intestine 223 56 90 8 179 29 neg*   382 54
D1469/06           
Spleen 484 426 57 4 38 7 neg*   67 67
Right kidney 235 23 10 8 14 3 3 3 111 29
Spinal Cord 289 64 11 1 126 33 neg*   14* 12
Pancreas 229 57 61 10 179 34 75 15 88* 14
Aorta lymph node 208 42 4 2 238 41 neg   112 11
Mammary Ln 355 299 140 26 297 12 465 60 112 18
  1. (Ln, lymph node; SD, standard deviation; neg, negative Brucella PCR assay; *, negative inhibition control).