Seroprevalence and risk factors of herpes simplex virus type-2 infection among pregnant women in Northeast India
© Biswas et al; licensee BioMed Central Ltd. 2011
Received: 7 December 2010
Accepted: 23 November 2011
Published: 23 November 2011
Herpes simplex virus type-2 (HSV-2) is one of the most common sexually transmitted infections that facilitate human immunodeficiency virus (HIV) acquisition by over two fold or more. The development of HSV-2 control methods as a measure to control HIV epidemic in high HSV-2/HIV areas has become a priority. Two out of the six high HIV prevalent states of India are located in the Northeastern region of India. Due to lack of documented HSV-2 studies from this part of the country; there was a need for estimating the seroprevalence and risk factors of HSV-2 infection in this defined population.
Pregnant women (n = 1640) aged18 years and above attending antenatal clinics of tertiary referral hospitals in five Northeastern states of India were screened for type specific HSV-2 IgG antibodies. Blood samples were collected from all the participants after conducting interviews. Univariate and multivariate analyses were performed to identify the risk factors associated with HSV-2 seropositivity.
Overall seroprevalence of HSV-2 infection was 8.7% (142/1640; 95% CI 7.3-10.0) with a highest prevalence of 15.0% (46/307; 95% CI 11.0-19.0) in the state of Arunachal Pradesh. Higher seroprevalence was observed with increasing age (Adj. Odds Ratio [AOR] 1.9 for 22-25 years old, AOR 2.29 for > 29 years old). The risk factors associated with HSV-2 seropositives were multiple sex partners (AOR 2.5, p = 0.04), condom non-user's (AOR 4.7, p < 0.001), early coitarchal age (age of first intercourse) 'less than 18 years' (AOR 9.6, p = 0.04), middle income group (AOR 2.1, p = 0.001) compared to low income group and low level of education (AOR 3.7, p = 0.02) compared to higher education. HSV-2 seropositivity was higher among Christians (12.6%) compared to Muslims (3.8%). The most frequent clinical symptoms among HSV-2 seropositives were excess vaginal discharge in last one year (53.5%, 76/142) and pelvic pain (26.1%, 37/142). While among subjects with genital ulcers, HSV-2 seroprevalence was 36.8% (7/19).
Overall seroprevalence of HSV-2 infection among pregnant women of Northeast India is relatively low. The generation of awareness among high risk groups may have played key role to limit the infection. The role of vaccination against HSV-2 in near future and elimination of HSV-2 viral shedding along with genital tract inflammation in high HIV/HSV-2 areas may be an option for initiating successful intervention strategies to reduce the transmission and acquisition of HIV infection in Northeast India.
Herpes Simplex Virus Type-2 (HSV-2) infections is almost always sexually transmitted and is the common cause of genital ulcer disease (GUD) worldwide [1, 2]. It is also a good marker of sexual behavior in the population [1, 2]. Seroprevalence studies show wide variations in infection rates by geographic location. Highest prevalence of HSV-2 has been found in some parts of Africa, America and the lowest in Asia . Globally about 535.5 million were infected with HSV-2 with an overall prevalence of 16.2% in 2003 . The HSV-2 prevalence in South-Asia in 2003 was estimated to be 29.4% and 33.2% among males and females in the age group of 15-49 years respectively . Recent study from India shows HSV-2 prevalence among general population to be around 10% . HSV-2 is gaining special attention as a significant risk factor for acquisition of human immunodeficiency virus type-1 (HIV-1). HSV-2 infection is also present in 30 to 70% of those in Europe and 50 to 90% of those in Africa among patients with HIV infection . HSV-2 increases the risk of HIV-1 transmission by more than two fold and HIV transmission on a per-sexual act basis by up to fivefold and may account for 40 - 60% of new HIV infections in high HSV-2 prevalent populations [7–14]. It is assumed that infection with HSV-2 disrupts the genital mucosa and provides a portal of entry for HIV, leading to increased susceptibility of HIV in HIV-negative persons. Also, HSV-2 is thought to enhance HIV acquisition due to dense inflammatory infiltrates of CD4/CD8/dendritic cells in the genital tract associated with HSV-2 shedding . Moreover, in HIV-positive persons, infection with HSV-2 accelerates replication and genital shedding of the virus, thus such individuals are more likely to transmit HIV [16, 17]. Increasing evidence of HSV-2 facilitating HIV acquisition and transmission makes the development of HSV control methods a priority [1, 6]. Also, other meta-analysis data from general population among women showed that in prevalent HSV-2 infection, HIV acquisition increased by over a three-fold . A study by Nagot et al. highlights the association of HSV-2 with significantly higher viral load of HIV-1 in plasma and in genital secretions in women with sexually acquired HIV-1 . This finding has direct clinical implications, suggesting that elimination of HSV-2 viral shedding and genital tract inflammation can reduce the transmission and acquisition of HIV infection.
The role of HSV-2 as a biological cofactor in HIV acquisition and transmission, which may have contributed substantially to HIV infections particularly by facilitating HIV spread among the low-risk population with stable long-term sexual partnerships . A study conducted in four cities of Africa indicated that in the two West African cities with lower HSV-2 prevalence, HIV remained concentrated in higher risk groups. However, in the cities with high HSV-2 prevalence, HIV transmission was observed in a large fraction of the population . These results suggest that HSV-2 may be the trigger for the explosive expansion of HIV and were also corroborated by the epidemiological synergy study of HIV and HSV-2 . The increasing evidence that HSV-2 facilitates HIV acquisition and transmission and the synergistic effect of HSV-2 on HIV, makes the development of HSV-2 control methods a priority.
In Northeast India, the epidemic of HIV-1 is driven mainly by the IDU population in the State of Manipur, Mizoram and Nagaland. The state of Manipur has the highest HIV prevalence in the country (1.67% in 2006) . In a recent study from this Institute, the HIV-1 prevalence was found to vary between 23%-32% among the IDUs of Manipur . Female sex workers (FSWs) are common in scattered locations in few northeast states of India. One recent study from this Institute has found HIV-1 prevalence of 13.6% in FSWs of Nagaland, a state in northeast India (unpublished data of the authors). The population of Northeast India is heterogeneous and there is a huge socio-cultural difference among the states. HIV preventive measures have been the cornerstone in the efforts to halt the epidemic amongst IDUs and FSWs of Northeast India. Over the years, HIV has made in-roads into the general population of the Northeastern states. The average HIV prevalence among women attending antenatal clinics in India is 0.48%. The HIV prevalence in the antenatal clinic attendees in the Northeastern state of Manipur was 1.25% and in Mizoram was 1.0% in 2006, which was among the highest in the country in this group of population .
Considering the above facts, this study was conducted to assess the seroprevalence of HSV-2 and its risk factors among pregnant women (antenatal clinic attendees) in five North-eastern states of India (viz. Assam, Arunachal Pradesh, Manipur, Meghalaya, and Mizoram). Since, more than 75% of primary genital herpes virus infections are asymptomatic , the seroepidemiological studies are critical in understanding the pattern and distribution of infection within the population to understand the HSV-2 related HIV transmission dynamic in the different communities of Northeast India.
Study area and Population
This is a cross-sectional study which was conducted in five, out of eight Northeastern states of India to understand the epidemiology and role of risk factors associated with HSV-2 seroprevalence with an aim focused towards prevention. A total of 1640 pregnant woman attending antenatal clinics (ANCs) in five tertiary referral hospitals representing the general population of each of the five Northeastern states i.e., Arunachal Pradesh, Assam, Manipur, Meghalaya, and Mizoram were enrolled in the study between April 2007 and March 2009. The present study was approved by institutional human ethical committee of the Regional Medical Research Centre. Trained field investigators had enrolled pregnant women aged 18 years and above who had consented for the study. Pregnant women in labor or under serious medical conditions were excluded from the study.
A predesigned and pretested structured questionnaire having information on socio-demographic variables such as age, religion, education, occupation, type of family, geographic location, annual income, gestational weeks, and risk factors that may be associated with sexually transmitted diseases like number of sex partners in previous one year, use of condom, alcohol consumption, coitarchal age, commercial sex worker, and type of sex partner were recorded. The clinical history, signs & symptoms associated with genito-pelvic infections, including previous history of genital ulcer, pelvic pain, and excessive vaginal discharge, pain during sexual intercourse, genital warts, and swelling in the groin were also recorded. The participants were interviewed confidentially and examined by qualified gynaecologists.
Specimen collection and laboratory test
Specimen of 5 ml venous blood were collected from all the enrolled subjects in K3 EDTA tubes and immediately kept at +4°C. Plasma samples were separated on the same day and stored at -20°C freezer in aliquots until analyzed. Plasma samples were subjected for HSV-2 IgG detection using type specific HSV-2 glycoprotein (gpG2) IgG EIA kit (HerpeSelect 2 ELISA IgG, Focus Diagnostics, USA) as per manufacture's manual. The cut-off value used to determine a positive test on this kit was > 1.10. Index value between 1.1 and 0.9 were considered equivocal and index values below 0.9 were considered negative as per the kit instruction manual. The equivocal samples were re-tested with the same kit. The samples that were found to be equivocal in the repeat test, a second samples were collected from the same subjects after 1-2 months for reconfirmation. All the positive samples and randomly selected 10% negative samples were re-tested for confirmation.
Data were entered and analyzed in SPSS version 13.0 statistical software (SPSS, Chicago, USA). The statistical significance of HSV-2 seroprevalence with other associated factors (socio-demographic, risk variables and clinical findings) was obtained using univariate analysis. The multivariate analysis was performed by binary logistic regression using Wald Backward elimination method with probability for stepwise entry at p = 0.05 and removal at p = 0.1, to find the association between categorical variables with HSV-2 seroprevalence. Odds ratios (OR) were calculated using Binary logistic regression analysis where first category (having lowest seroprevalence) was used as a reference in each group. OR and their 95% confidence intervals (95% CI) were documented to indicate magnitude and direction of associations. The variables showing significant interactions were analyzed separately. The level of statistical significance was set at p ≤ 0.05.
A total of 1640 pregnant women from five Northeastern states of India were studied. The median age of the study subjects was 25 years (SD ± 4.7) and ranged from 18 to 46 years. The literacy rate of the enrolled subjects was 84.5% (n = 1386), among which 49.3% (n = 809) were educated up to primary level. Majority of the subjects were housewife 79.9% (n = 1310), lived in nuclear family 57.9% (n = 950), and were from low income group 71.8% (n = 1177). Within the enrolled subjects, 67.0% (n = 1099) were in 3rd trimester of pregnancy and 43.2% (n = 708) were primi-gravida with a median of two gravida (2 ± 1.8).
State wise socio-demographic variables of the study subjects, Northeast India (n = 1640).
Subjects recruited (1640)
18 to 21 yrs (355)
22 to 25 yrs (592)
26 to 29 yrs (385)
> 29 yrs (308)
House wife (1310)
Regular employee (110)
Unskilled worker (75)
Primary & less (1063)
Graduate & above (115)
Type of family
Middle & high (463)
Migrant labor (192)
Duration of Pregnancy
1-12 weeks (28)
13-28 weeks (513)
29-42 weeks (1099)
Commercial sex worker (8)
Risk factors associated with HSV-2 seroprevalence among pregnant women in Northeast India (n = 1640).
18 to 21 yrs (355)
22 to 25 yrs (592)
26 to 29 yrs (385)
> 29 yrs (308)
Arunachal Pradesh (307)
Unskilled worker (75)
House wife (1310)
Regular employee (110)
Graduate & above (115)
Primary & less (1063)
Type of family
> 30 yrs (29)
18 to 30 yrs (1335)
< 18 yrs (218)
No response (58)
Never user (1267)
Regular & occasional users (373)
More than 1 (39)
Regular & occasional users (341)
Never user (1299)
The univariate and multivariate analysis of risk factors associated with HSV-2 seroprevalence is presented in Table 2. In univariate analysis, it was found that HSV-2 seroprevalence differs significantly (p < 0.001) among the subjects of different religion in North-east India. Subjects from Christian community were at higher risk (AOR 3.5, 95% CI 1.2-10.1, p = 0.02) of getting infection with reference to Muslims (analyzed separately because of significant interaction with 'state'). On multivariate analysis, it was found that subjects in the state of Arunachal Pradesh has the highest risk of HSV-2 compared to the state of Manipur (AOR 11.5, 95% CI 5.2-25.5, p < 0.001). The risk-factors that had a significant association with HSV-2 were condom never users compared to regular or occasional users (AOR, 4.7, 95% CI 2.5-8.9, p < 0.001); coitarchal age less than 18 years (AOR 9.6, 95% CI 1.1-81.6, p = 0.04); more than one sex partner (AOR 2.5, 95% CI 1.1-6.1, p = 0.04); middle or high income compared to low income subjects (AOR 2.2, 95% CI 1.4-3.3, p = 0.001); subjects from nuclear family (AOR 1.7, 95% CI 1.1-2.6, p = 0.02); subjects with low level of education (AOR 3.7, 95% CI 1.3-10.7, p = 0.02) and subjects having regular earning through a regular job (AOR 3.4, 95% CI 1.3-8.8, p = 0.01) [see table 2]. Though HSV-2 seroprevalence was higher amongst alcoholics (12.1%) compared to non-alcoholics (7.7%), it was not significant in multivariate analysis.
Association of clinical sign & symptoms with HSV-2 seroprevalence among pregnant women of Northeast India (n = 1640).
HSV-2 Sero prevalence
Excess vaginal discharge
The conceptual importance of this study was largely based on the increasing evidence that HSV-2 infection has a synergistic activity on acquiring HIV infection and this has been well documented [6–14, 16, 17]. Since there were virtually no data on the prevalence of HSV-2 in the community of Northeastern states of India, there was a need to know the prevalence of this infection to plan an intervention strategy especially in the high HIV prevalent states of Northeast India. This sero-epidemiological study was based on detection of HSV-2 IgG antibodies against type specific glycoprotein G2. The HerpeSelect HSV-2 IgG ELISA test (Focus Diagnostics), in an evaluation study demonstrated a sensitivity of 96% in a group of pregnant women and 95% in a STD population of men and women. The specificity of Focus HSV-2 ELISA was also high in these groups i.e. 97% in pregnant women and 96% in the STD population . The relative specificity of the assay as compared to Western Blot assay (Gold standard) in a low prevalent population was shown to be 98.7% and relative sensitivity 100% as per instruction manual of the kit. Though, the kit used in our study has a very high sensitivity and specificity, the false positivity of HSV-2 in low prevalence area cannot be ruled out. We also acknowledge the limitations of statistical test used in this cross-sectional study which may be prone to residual confounding effect in comparison to case control studies.
Our study detected a significant geographical variation of HSV-2 seroprevalence which has also been observed in an earlier study . Community based studies conducted elsewhere in India also reported wide variations in seroprevalence [3, 25]. One study from Chennai based on study of around 30 communities, reported a wide variation in prevalence of HSV-2 from 4.1 to 49.1% with a mean prevalence of 15.3% . Studies conducted among the high risk groups showed highest seroprevalence (40% to 70%) [12, 26–28]. In our study, we expected highest HSV-2 prevalence in the state of Manipur, which has the highest HIV prevalence in India and expected lowest in the state of Arunachal Pradesh which has the lowest HIV prevalence in the country . But to the contrary, we detected the lowest HSV-2 seroprevalence in the state of Manipur (2.7%) and highest in the state of Arunachal Pradesh (15%). This may be the result of the widespread public awareness in the state of Manipur in last two decades on practice of safe sex to fight HIV epidemic in that state, whereas such public awareness drive is virtually absent in the state of Arunachal Pradesh.
In comparison to other National and international studies, our finding of 8.7% HSV-2 seroprevalence was relatively low. A community based study in New Delhi reported 8.6% seroprevalence among females, which is similar to our findings . Higher seroprevalence were reported from Chennai (15.3%) in South India , central India (12.4%)  and Gujarat (23.3%) in Western India among women attending gynaecology OPD . Seroprevalence of 7.5% among pregnant women from Jammu & Kashmir, North India , and 7.0% among females from Andhra Pradesh, South India was reported in a community based study . The National Health and Nutrition Examination Survey (NHANES) surveillance data from USA found HSV-2 seroprevalence of 20.9% in US females aged '14 to 49 years', with an overall seroprevalence of 16.2% . The HSV-2 seroprevalence among 15 to 49 years females in South Asian region was reported to be 33.2%, whereas it was 19.4% globally .
In comparison to low income group, we observed significantly higher HSV-2 seroprevalence among middle or high income group. Studies in India and abroad also observed association of socio-economic status (SES) with HSV-2 seroprevalence [3, 25, 34]. This has been observed from both the previously mentioned study from New Delhi , and Chennai . In the study from New Delhi, HSV-2 seroprevalence was found to be more common in the urban middle class community with higher average monthly income (12.6%) as compared to urban slum with lower average monthly income (3.1%) . While, the study from Chennai reported an independent association of community-level factors like SES with HSV-2 sero-prevalence . In another study from Australia, found that HSV-2 seroprevalence was significantly lower in areas of low SES than in high SES areas among both men and women for all ages . However, some other studies have reported significant association between HSV-2 seropositivity and lower income . Though this paradox would be hard to explain, it may be related to difference in risk behavior among the different income groups. It was seen in our study that majority of Muslim subjects (84.9%, 90/106) were from low income group. It was also observed that Muslim subjects had the lowest HSV-2 seroprevalence (3.8%) compared to Hindu (5.8%) and Christians (12.6%), which may explain this finding in our study.
It is generally being observed that HSV-2 seroprevalence increases with increasing age [25, 33, 36, 37]. In our study we also observed an increase in prevalence with age (6.5% to 9.4%) but with a peak in prevalence (10.5%) in the 22-25 years age group. This may reflect a relatively recent introduction of HSV-2 in the community which may have resulted in higher prevalence among sexually active younger subjects rather than the older subjects which didn't had the exposure to the virus when they were sexually active in the past.
The risk factors for HSV-2 are well known and include polygamous relationships/multiple sex partners', unsafe sex, increasing numbers of lifetime partners and association with other STIs or GUD etc [7, 16, 35, 38]. The strongest predictor for infection is a person's number of lifetime sex partners . In our study, it was also observed that subjects with multiple sex partners had significantly higher HSV-2 seroprevalence compared to single sex partner. Moreover, it was seen that higher coitarchal age was associated with a lower chance of HSV-2 infection. The association of coitarchal age less than 18 years or early age of first sex with seroprevalence was also observed in other studies [39, 40]. In this study, higher seroprevalence was found among Christians versus Muslims. This difference in prevalence with religion (3.8% in Muslim compared to 12.6% among Christians), may be due to practice of male circumcision at infancy or early childhood by the spouses of the pregnant women among Muslims. Male circumcision lowers the prevalence of HSV-2 or HPV has been reported in some studies . Subjects from nuclear family, middle income group, low level of education, having regular source of income, had a higher risk of HSV-2 infections. Modifiable risk factors included, low coitarchal age, never or low condom usage, and multiple sex partners, which can be incorporated in awareness programs to lower the transmission of HSV-2 in the community.
Our study found a significantly higher HSV-2 seropositive amongst subjects with sign and symptoms of genito-pelvic infections such as genital ulcer, pelvic pain and cervicitis. In our study it was found that HSV-2 prevalence was higher among subjects with excessive vaginal discharge but was not significant statistically. The subjects with history of genital ulcers had the highest HSV-2 seroprevalence among the study variables. Also, it is known that majority of HSV2 seropositive people shed virus whether or not they are aware of recurrent genital lesions. Genital tract inflammation is evident in "asymptomatic shedders" . Studies conducted in Surat and Delhi also observed higher HSV-2 seroprevalence in subjects with vaginal discharge and lower abdominal or low backache [30, 43].
In Northeast India, the impact of HSV-2 infection in driving the HIV-1 epidemics is still not clear. Although HSV-2 vaccines are still in clinical trials, acyclovir when given continuously or episodically can reduce recurrence of ulcers in individuals with HSV-2 infection. Though clinical trials in Africa on use of acyclovir did not reduce the risk of linked transmission of HIV-1 to couples, however it showed a reduction of plasma HIV-1 RNA level as well as 73% reduction in occurrence of genital ulcers due to HSV-2 . Multiple studies have shown that a persistent increase of 0.5 log10 copy per millilitre in the plasma HIV-1 level is associated with a clinically shortened time for progression to AIDS . The demonstration that daily anti-HSV-2 therapy can reduce the viral load by this amount is thus of direct importance for treatment. The study by Nagot et al. underlines the findings of a 1989 study showing that zidovudine plus acyclovir (ACV) was associated with prolonged survival, as compared with zidovudine alone . The data from another controlled trial with HSV-2 suppressive therapy with acyclovir showed no evidence of decreasing the incidence of HIV in Tanzania . This landmark study fails to demonstrate the role of acyclovir in decreasing the transmission of HIV among HSV-2 seropositives. This is in contrary to the fact that acyclovir is activated into a human herpes virus (HHV) DNA polymerase inhibitor exclusively by HHV kinases. It suppresses HIV-1 in HHV coinfected human tissues but not in HHV free tissue or cell cultures . Acyclovir treatment in patients coinfected with HSV/HIV has been observed to alter the disease course and decrease HIV viral load which attributed to indirect effects of HSV suppression on HIV replication . Also, if we accept the fact that HSV-2 increases the risk of HIV acquisition by 2-3 folds, than suppression of HSV-2 by ACV which results in decrease genial ulceration and inflammation would be expected to influence HIV acquisition. Use of antivirals for suppression of HSV-2 for lowering the risk of HIV transmission cannot be overlooked until more light is shed on this topic. Therapeutic options that provide a complete eradication of HSV-2 including suppression of genital inflammation may be more relevant for decreasing HIV transmission in high HSV-2 prevalent regions.
The findings of this study which is first of its kind in North-east India, has provided a baseline data on HSV-2 seroprevalence and risk factors for future in-depth studies. The data provides a useful epidemiologic signal for a possible impending spike in HIV incidence among the young age group (22-25 years) as is indicated by a higher HSV-2 prevalence in this age group. Targeted public health awareness campaigns directed at high risk groups having multiple sexual partners, condom non-users, early coitarchal age, Christian community etc. are needed to be advocated for initiating successful intervention strategies to control HSV-2 and indirectly limiting HIV epidemic in Northeast India.
We are thankful to Dr. B. C. Das, ICPO, Noida for providing us hands on training on various aspects of the study and Dr. Kanwar Narain of RMRC, Dibrugarh for his statistical support. We are also grateful to Dr. (Mrs.) Angshurekha Das, Dr. (Mrs.) Gargi Choudhuri, all the study participants and project staffs for their support in the study without which this study would have not been possible. This study was funded by Indian Council of Medical Research.
- WHO and UNAIDS: Herpes simplex virus type 2: programmatic and research priorities in developing countries. Report of a WHO/UNAIDS/LSHTM WORKSHOP, London. 2001, WHO/HIV_AIDS/2001.05.2001, , 14-16 February
- Smith JS, Robinson NJ: Age-specific prevalence of infection with herpes simplex virus types 2 and 1: A global review. J Infect Dis. 2002, 186 (Suppl1): S3-28.View ArticlePubMed
- Jennings JM, Louis TA, Ellen JM, Srikrishnan AK, Sivaram S, Mayer K, Solomon S, Kelly R, Celentano DD: Geographic Prevalence and Multilevel Determination of Community-level Factors Associated with Herpes Simplex Virus Type 2 Infection in Chennai, India. Am J Epidemiol. 2008, 167 (12): 1495-1503. 10.1093/aje/kwn066.View ArticlePubMed
- Looker KJ, Garnetta GP, Schmid GP: An estimate of the global prevalence and incidence of herpes simplex virus type 2 infection. WHO Bulletin. 2008, 86 (10): 805-13.
- Sgaier SK, Mony P, Jaykumar S, McLaughlin C, Arora P, Kumar R, Bhatia P, Jha P: Prevalence and correlates of Herpes Simplex Virus-2 and syphilis infections in the general population in India. Sex Transm Infect. 2011, 87: 94-100. 10.1136/sti.2010.043687.View ArticlePubMed
- Corey L: Synergistic Co pathogens - HIV-1 and HSV-2. N Engl J Med. 2007, 356 (8): 854-856. 10.1056/NEJMe068302.View ArticlePubMed
- Mbizvo EM, Msuya Sia E, Stray-Pedersen B, Chirenje MZ, Munjoma M, Hussain A: Association of herpes simplex virus type 2 with human immunodeficiency virus among urban women in Zimbabwe. Int J STD AIDS. 2002, 13: 343-8. 10.1258/0956462021925171.View ArticlePubMed
- Wald A, Link K: Risk of human immunodeficiency virus infection in herpes simplex virus type 2-seropositive persons: a meta-analysis. J Infect Dis. 2002, 185 (1): 45-52. 10.1086/338231.View ArticlePubMed
- Serwadda D, Gray RH, Sewankambo NK, Wabwire-Mangen F, Chen MZ, Quinn TC, Lutalo T, Kiwanuka N, Kigozi G, Nalugoda F, Meehan MP, Morrow RA, Wawer MJ: Human immunodeficiency virus acquisition associated with genital ulcer disease and herpes simplex virus type 2 infection: a nested case-control study in Rakai, Uganda. J Infect Dis. 2003, 188 (10): 1492-7. 10.1086/379333.View ArticlePubMed
- Holmberg SD, Stewart JA, Gerber AR, Byers RH, Lee FK, O'Malley PM, Nahmias AJ: Prior Herpes Simplex Virus Type 2 Infection as a Risk Factor for HIV Infection. JAMA. 1988, 259: 1048-1050. 10.1001/jama.1988.03720070048033.View ArticlePubMed
- Holmberg SD, Gerber AR, Stewart JA, Lee FK, O'Malley PM, Nahmias AJ: Herpes viruses as co-factors in AIDS. Lancet. 1988, 2: 746-747.View ArticlePubMed
- Reynolds SJ, Risbud AR, Shepherd ME, Zenilman JM, Brookmeyer RS, Paranjape RS, Divekar AD, Gangakhedkar RR, Ghate MV, Bollinger RC, Mehendale SM: Recent herpes simplex virus type 2 infection and the risk of human immunodeficiency virus type 1acquisition in India. J Infect Dis. 2003, 187: 1513-21. 10.1086/368357.View ArticlePubMed
- Corey L: The current trend in genital herpes. Progress in prevention. Sex Transm Dis. 1994, 21 (suppl 2): S38-44.PubMed
- Corey L, Wald A, Celum CL, Quinn TC: The effects of herpes simplex virus-2 on HIV-1 acquisition and transmission: a review of two overlapping epidemics. J Acquir Immune Defic Syndr. 2004, 35: 435-445. 10.1097/00126334-200404150-00001.View ArticlePubMed
- Zhu J, Hladik F, Woodward A, Klock A, Peng T, Johnston C, Remington M, Magaret A, Koelle DM, Wald A, Corey L: Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisition. Nat Med. 2009, 15: 886-892. 10.1038/nm.2006.PubMed CentralView ArticlePubMed
- Yahya-Malima Khadija, Bjørg Evjen-Olsen, Matee Mecky, Knut Fylkesnes, Lars Haarr: HIV-1, HSV-2 and syphilis among pregnant women in a rural area of Tanzania: Prevalence and risk factors. BMC Infect Dis. 2008, 8: 75-10.1186/1471-2334-8-75.PubMed CentralView ArticlePubMed
- Nagot N, Ouédraogo A, Foulongne V, Konaté I, Weiss HA, Vergne L, Defer MC, Djagbaré D, Sanon A, Andonaba JB, Becquart P, Segondy M, Vallo R, Sawadogo A, Perre PVD, Mayaud P: Reduction of HIV-1 RNA levels with therapy to suppress herpes simplex virus. N Engl J Med. 2007, 356: 790-799. 10.1056/NEJMoa062607.View ArticlePubMed
- Freeman EE, Weiss HA, Glynn JR, Cross PL, Whitworth JA, Hayes RJ: Herpes simplex virus 2 infection increases HIV acquisition in men and women: systematic review and meta-analysis of longitudinal studies. AIDS. 2006, 20 (1): 73-83. 10.1097/01.aids.0000198081.09337.a7.View ArticlePubMed
- Abu-Raddad LJ, Magaret AS, Celum C, Wald A, Longini IM, Self SG, Cory L: Genital herpes has played a more important role than any other sexually transmitted infection in driving HIV prevalence in Africa. PLoS ONE. 2008, 3 (5): e2230-10.1371/journal.pone.0002230.PubMed CentralView ArticlePubMed
- Buvé A, Caraël M, Hayes RJ, Auvert B, Ferry B, Robinson NJ, Anagonou S, Kanhonou L, Laourou M, Abega S, Akam E, Zekeng L, Chege J, Kahindo M, Rutenberg N, Kaona F, Musonda R, Sukwa T, Morison L, Weiss HA, Laga M: The multicentre study on factors determining the differential spread of HIV in four African cities: summary and conclusions. AIDS. 2001, 15 (Suppl 4): S127-S131.View ArticlePubMed
- National AIDS Control Organization. Accessed 28 April 2011., [http://www.nacoonline.org/upload/NACO%20PDF/Note%20on%20HIV%20Sentinel%20Surveillance%20and%20HIV%20Estimation_01%20Feb%2008.pdf]
- Mahanta J, Medhi GK, Paranjape RS, Roy N, Kohli A, Akoijam SB, Dzuvichu B, Das HK, Goswami P, Thongamba G: Injecting and sexual behaviors, sexually transmitted infections and HIV prevalence in injecting drug users in three states in India. AIDS. 2008, 22 (Suppl 5): S59-S68. 10.1097/01.aids.0000343764.62455.9e.View ArticlePubMed
- Ural Serdar: Genital Herpes in Pregnancy. Accessed 18 November 2011., [http://emedicine.medscape.com/article/274874-overview]
- Ashly RL: Performance and use of HSV type-specific serology test kits. Herpes. 2002, 9 (2): 38-45.
- Chawla R, Bhalla P, Bhalla K, Singh MM, Garg S: Community-based study on seroprevalence of herpes simplex virus type 2 infection in New Delhi. Indian J Med Microbiol. 2008, 26 (1): 34-9. 10.4103/0255-0857.38855.View ArticlePubMed
- Wang H, Wang N, Chen RY, Sharp GB, Ma Y, Wang G, Ding G, Wu Z: Prevalence and predictors of herpes simplex virus type 2 infections among female sex workers in Yunnan Province, China. Int J STD AIDS. 2008, 19 (9): 635-639. 10.1258/ijsa.2008.008013.PubMed CentralView ArticlePubMed
- Watson-Jones D, Weiss HA, Rusizoka M, Baisley K, Mugeye K, Changalucha J, Everett D, Balira R, Knight L, Ross D, Hayes RJ: Risk Factors for Herpes Simplex Virus Type 2 and HIV Among Women at High Risk in Northwestern Tanzania: Preparing for an HSV-2 Intervention Trial. J Acquir Immune Defic Syndr. 2007, 46 (5): 631-642. 10.1097/QAI.0b013e31815b2d9c.PubMed CentralView ArticlePubMed
- Glynn JR, Crampin AC, Ngwira BMM, Ndhlovu R, Mwanyongo O, Fine PEM: Herpes simplex virus type 2 trends in relation to the HIV epidemic in northern Malawi. Sex Transm Infect. 2008, 84: 356-360. 10.1136/sti.2008.030056.PubMed CentralView ArticlePubMed
- Anvikar AR, Rao VG, Savargaonkar DD, Rajiv Y, Bhondeley MK, Tiwari B, Karkare A, Luke C, Gadge V, Ukey M, Patel P: Seroprevalence of sexually transmitted viruses in the tribal population of Central India. Int J Infect Dis. 2009, 13: 37-39. 10.1016/j.ijid.2008.03.021.View ArticlePubMed
- Mitra N, Gupta N: Seroprevalence and correlates of herpes simplex virus type-2 infection in a general gynaecology clinic. Arch Gynecol Obstet. 2007, 275: 19-23.View Article
- Rathore S, Jamwal A, Gupta V: Herpes simplex virus type 2: Seroprevalence in antenatal women. Indian J Sex Transm Dis. 2010, 31: 11-5. 10.4103/0253-7184.68994.PubMed CentralView ArticlePubMed
- Schneider JA, Lakshmi V, Dandona R, Kumar GA, Sudha T, Dandona L: Population-based seroprevalence of HSV-2 and syphilis in Andhra Pradesh state of India. BMC Infect Dis. 2010, 10: 59-PubMed CentralView ArticlePubMed
- Xu F, Sternberg MR, Gottlieb SL, Berman SM, Markowitz LE, Forhan SE, Taylor LD: Seroprevalence of Herpes Simplex Virus Type 2 among Persons Aged 14-49 Years - United States, 2005-2008. MMWR. 2010, 59 (15): 456-459.
- Page A, Taylor R, Richters J, Shaw J, Taylor J, Cunningham A, Mindel A: Upstairs and downstairs: socio-economic and gender interactions in herpes simplex virus type 2 seroprevalence in Australia. Sex Transm Dis. 2009, 36 (6): 344-9. 10.1097/OLQ.0b013e3181990c9a.View ArticlePubMed
- Buchacz K, McFarland W, Hernandez M, Klausner JD, Page-Shafer K, Padian N, Molitor F, Ruiz JD, Bolan G, Morrow S, Katz MH: Prevalence and correlates of herpes simplex virus type 2 infection in a population-based survey of young women in low-income neighborhoods of Northern California. Sex Transm Dis. 2000, 27: 393-400. 10.1097/00007435-200008000-00004.View ArticlePubMed
- Siegel D, Golden E, Washington AE, Morse SA, Fullilove MT, Catania JA, Marin B, Hulley SB: Prevalence and correlates of herpes simplex infection: The population-based AIDS in Multiethnic Neighborhoods Study. JAMA. 1992, 268: 1702-8. 10.1001/jama.1992.03490130090036.View ArticlePubMed
- Weiss HA, Buvé A, Robinson NJ, Van Dyck E, Kahindo M, Anagonou S, Musonda R, Zekeng L, Morison L, Caraël M, Laga M, Hayes RJ: The epidemiology of HSV-2 infection and its association with HIV infection in four urban African populations. AIDS. 2001, 15 (Suppl 4): S97-S108.View ArticlePubMed
- Obasi A, Mosha F, Quigley M, Sekirassa Z, Gibbs T, Munguti K, Todd J, Grosskurth H, Mayaud P, Changalucha J, Brown D, Mabey D, Hayes R: Antibody to herpes simplex virus type 2 as a marker of sexual risk behavior in rural Tanzania. J Infect Dis. 1999, 179 (1): 16-24. 10.1086/314555.View ArticlePubMed
- Nilsen A, Mwakagile D, Marsden H, Langeland N, Matre R, Haarr L: Genital Herpes Simplex Virus Infection. Forum for Nord Derm Ven. 2008, 46: Dissertations Vol. 13 (2)
- Tideman RL, Taylor J, Marks C, Seifert C, Berry G, Trudinger B, Cunningham A, Mindel A: Sexual and demographic risk factors for herpes simplex type 1 and 2 in women attending an antenatal clinic. Sex Transm Infect. 2001, 77: 413-415. 10.1136/sti.77.6.413.PubMed CentralView ArticlePubMed
- Tobian AAR, Serwadda D, Quinn TC, Kigozi G, Gravitt PE, Laeyendecker O, Charvat B, Ssempijja V, Riedesel M, Oliver AE, Nowak RG, Moulton LH, Chen MZ, Reynolds SJ, Wawer MJ, Gray RH: Male circumcision for the prevention of HSV-2 and HPV infections and syphilis. N Engl J Med. 2009, 360: 1298-1309. 10.1056/NEJMoa0802556.PubMed CentralView ArticlePubMed
- Wald A, Zeh J, Selke S, Warren T, Ryncarz AJ, Ashley R, Krieger JN, Corey L: Reactivation of Genital Herpes Simplex Virus Type 2 Infection in Asymptomatic Seropositive Persons. N Engl J Med. 2000, 342: 844-850. 10.1056/NEJM200003233421203.View ArticlePubMed
- Ray K, Bala M, Bhattacharya M, Muralidhar S, Kumari M, Salhan S: Prevalence of RTI/STI agents and HIV infection in symptomatic and asymptomatic women attending peripheral health set-ups in Delhi, India. Epidemiol Infect. 2008, 136 (10): 1432-1440.PubMed CentralView ArticlePubMed
- Celum C, Wald A, Lingappa JR, Magaret AS, Wang RS, Mugo N, Mujugira A, Baeten JM, Mullins JI, Hughes JP, Bukusi EA, Cohen CR, Katabira E, Ronald A, Kiarie J, Farquhar C, Stewart GJ, Makhema J, Essex M, Were E, Fife KH, Bruyn GD, Gray GE, McIntyre JA, Manongi R, Kapiga S, Coetzee D, Allen S, Inambao M, Kayitenkore K, et al: Acyclovir and Transmission of HIV-1 from Persons Infected with HIV-1 and HSV-2. N Engl J Med. 2010, 362: 427-439. 10.1056/NEJMoa0904849.PubMed CentralView ArticlePubMed
- Watson-Jones D, Weiss HA, Rusizoka M, Changalucha J, Baisley K, Mugeye K, Tanton C, Ross D, Everett D, Clayton T, Knight L, Hambleton I, Goff JL, Belec L, Hayes R: Effect of Herpes Simplex Suppression on Incidence of HIV among Women in Tanzania. N Engl J Med. 2008, 358: 1560-1571. 10.1056/NEJMoa0800260.PubMed CentralView ArticlePubMed
- Lisco A, Vanpouille C, Tchesnokov EP, Grivel JC, Biancotto A, Brichacek B, Elliott J, Fromentin E, Shattock R, Anton P, Gorelick R, Balzarini J, McGuigan C, Derudas M, Gotte M, Schinazi RF, Margolis L: Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpes virus-infected human tissues. Cell Host Microbe. 2008, 4: 260-270. 10.1016/j.chom.2008.07.008.PubMed CentralView ArticlePubMed
- McMahon MA, Siliciano JD, Lai J, Liu JO, Stivers JT, Siliciano RF, Kohli RM: The Antiherpetic Drug Acyclovir Inhibits HIV Replication and Selects the V75I Reverse Transcriptase Multidrug Resistance Mutation. J Biol Chem. 2008, 283: 31289-31293. 10.1074/jbc.C800188200.PubMed CentralView ArticlePubMed
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/11/325/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.