Staphylococcus aureus bacteriuria as a prognosticator for outcome of Staphylococcus aureus bacteremia: a case-control study
© Chihara et al; licensee BioMed Central Ltd. 2010
Received: 13 July 2009
Accepted: 29 July 2010
Published: 29 July 2010
When Staphylococcus aureus is isolated in urine, it is thought to usually represent hematogenous spread. Because such spread might have special clinical significance, we evaluated predictors and outcomes of S. aureus bacteriuria among patients with S. aureus bacteremia.
A case-control study was performed at John H. Stroger Jr. Hospital of Cook County among adult inpatients during January 2002-December 2006. Cases and controls had positive and negative urine cultures, respectively, for S. aureus, within 72 hours of positive blood culture for S. aureus. Controls were sampled randomly in a 1:4 ratio. Univariate and multivariable logistic regression analyses were done.
Overall, 59% of patients were African-American, 12% died, 56% of infections had community-onset infections, and 58% were infected with methicillin-susceptible S. aureus (MSSA). Among 61 cases and 247 controls, predictors of S. aureus bacteriuria on multivariate analysis were urological surgery (OR = 3.4, p = 0.06) and genitourinary infection (OR = 9.2, p = 0.002). Among patients who died, there were significantly more patients with bacteriuria than among patients who survived (39% vs. 17%; p = 0.002). In multiple Cox regression analysis, death risks in bacteremic patients were bacteriuria (hazard ratio 2.9, CI 1.4-5.9, p = 0.004), bladder catheter use (2.0, 1.0-4.0, p = 0.06), and Charlson score (1.1, 1.1-1.3, p = 0.02). Neither length of stay nor methicillin-resistant Staphylococcus aureus (MRSA) infection was a predictor of S. aureus bacteriuria or death.
Among patients with S. aureus bacteremia, those with S. aureus bacteriuria had 3-fold higher mortality than those without bacteriuria, even after adjustment for comorbidities. Bacteriuria may identify patients with more severe bacteremia, who are at risk of worse outcomes.
Bacteriuria with Staphylococcus aureus is postulated to occur through a limited number of mechanisms--primarily ascending spread after instrumentation (e.g., urologic procedures or urethral catheterization) or hematogenous seeding of the genitourinary tract. The finding by Lee et al., that bacteremia is strongly associated with bacteriuria among patients infected with S. aureus, supports the notion that bacteremia is an important precursor for bacteriuria . What is less clear, however, is whether in patients with S. aureus bacteremia, the finding of S. aureus in the urine holds any prognostic significance.
Among patients with S. aureus bacteremia, there are many prognostic factors, including host factors or comorbidities (age [2–10], Charlson score [2, 11], immunosuppression , alcoholism , hemodialysis , acute renal failure , diabetes mellitus , recent hospitalization , mechanical ventilation , and acute severity of illness ); pathogen-specific factors (MRSA [3, 13] superantigenic toxin production ); or characteristics of clinical presentation or management (meningitis  or altered mental status [11, 12], community-acquired infection , severe sepsis or septic shock [3, 4, 6, 7], lack of an infectious disease consult [4, 15], prior antibiotic therapy , unknown  or persisting foci of infection , daily dose of penicillinase-stable penicillin < 4 grams , and inappropriate empiric treatment [6, 12] or duration of treatment < 14 days ). The relation of occurrence of bacteriuria to outcome has been postulated , but the interactions with other risk factors warrant investigation. That bacteriuria might be a predictor of worse outcome in S. aureus bacteremia has biologic plausibility. For example, several innate defense mechanisms exist that prevent the development of urinary tract seeding in bacteremic patients: defensins, Toll-like receptor 4, and chemokine receptor CXCR1 [18, 19], and these innate defenses may be overcome and the urinary tract seeded in the setting of higher bacterial loads .
Over the last 20 years, MRSA has emerged as an important cause of nosocomial bacteremia , and significant increases in the incidence of MRSA infections in community patients have been observed in the past few years [22–24]. Methicillin-resistance is a major risk for increased morbidity and mortality in S. aureus infection. Given the increasing burden of infection by community-associated MRSA (CA-MRSA) infections [22–24] and the suggestion that CA-MRSA strains may have greater virulence, predictors of morbidity and mortality are essential for allocation of clinical infections.
We hypothesized that for patients with S. aureus bacteremia, bacteriuria is an important marker of disease severity and predictor of worse outcomes. We examined this hypothesis by evaluating outcomes of S. aureus bacteremia in patients with and without bacteriuria. We also examined the impact of methicillin-resistance on outcomes among patients with S. aureus bacteremia with and without bacteriuria. Finally, using our cohort, we examined the predictors of bacteriuria.
A case-control study was performed for patients admitted to John H. Stroger Jr. Hospital of Cook County, a 464-bed inner-city safety-net hospital in Chicago, Illinois. The institutional review board reviewed the study and deemed it exempt from review.
Study Design and Definitions
Our study included in-patients during January 2002 through December 2006. Cases were patients who had a positive urine culture for S. aureus within 72 hours of a positive blood culture for S. aureus. Controls were patients who had a negative urine culture for S. aureus within 72 hours of a positive blood culture S. aureus. Control patients were randomly sampled from all eligible patients with S. aureus bacteremia but without bacteriuria (i.e., with negative urine cultures), with the aim of 4 controls per case. Subsequently, chart reviews were performed and exclusion criteria were applied to sampled putative cases and controls to create a cohort for analysis.
Patients who were younger than age 18 years, who were seen only in the emergency department or in the clinic, or who did not have a urine culture performed within 72 hours of the positive blood cultures, were excluded. Only the first episode of S. aureus bacteremia per admission was assessed.
Using electronic data and chart review, we collected demographic and clinical data including mortality, ICU admission, length of stay, age, race, sex, Charlson score , comorbidities (HIV, diabetes mellitus, end stage renal disease requiring hemodialysis), alcoholism, intravenous drug use, presence of indwelling bladder catheter at the time of culture, previous urological procedure, duration of fever, duration of bloodstream infection, urinary symptoms, duration of chief complaint, organs and sites infected by S. aureus decided by the clinician, evidence of foreign material, susceptibilities of S. aureus, and time to appropriate antimicrobial therapy. For patients who died during their hospitalization, death was attributed to the bacteremia if so noted by the patient's discharge diagnosis or when there was a positive blood culture within 1 week preceding death.
S. aureus was deemed hospital-acquired if illness had onset > 72 hours after hospital admission. Cultures that turned positive within 72 hours of admission--community-onset--were divided into 2 groups: healthcare-associated infection was defined based on history of hospitalization, surgery, dialysis, or residence in a long-term care facility within 1 year or a presence of an indwelling catheter or percutaneous medical device (e.g., tracheostomy tube, gastrostomy tube, or bladder catheter) or a prior MRSA infection; patients with community-acquired infection did not have any of these risk factors .
Categorical variables were examined with chi-square analysis and continuous variables were analyzed with the independent samples t test. For multivariable analysis, statistically important factors with p values <0.10 on univariate analysis were included in initial models, and Charlson score was included a priori based on the strong association between comorbidities and death, and our desire to appropriately adjust for comorbidities in our analysis. We used Cox proportional hazards regression analysis to assess the association of bacteriuria with time to S. aureus death or hospital discharge, adjusted for suspected effect modifiers as identified in univariate analysis. Explanatory variables were evaluated for adherence to the proportional hazards assumption. To assess the multivariable predictors of bacteriuria, logistic regression was used. Data analysis was performed using SPSS software (version 15.0, Chicago, IL).
During 5 years (January 1, 2002, through December 31, 2006), 67 patients with S. aureus bacteremia and bacteriuria were identified, and control sampling yielded 268 bacteremic, non - bacteriuric patients. Following chart reviews and application of exclusion criteria, there were 61 cases and 247 controls. Of all 308 episodes of bacteremia, 128 (41.6%) were due to MRSA.
The mean age of pooled dataset, created by joining case and control patients was 48.3 years; 68.5% were male, 181 (58.8%) were African-American, and 37 (12.0%) died during hospital admission. Sites involved by bacteremia were cardiac valves [31 (10.7%)], vascular catheters [59 (19.2%)], skin/soft tissue [57 (18.5%)], bone/joints [27 (8.8%)], lungs [41 (13.3%)], genitourinary tracts [22 (7.1%)], and other sites [11 (3.6%)]; for 106 (36.7%) patients, there was no documented organ involvement other than bloodstream.
Univariate analysis of predictors of death during admission among patients with S. aureus bacteremia and a contemporaneous urine culture obtained a
Death during admission
No death during admission
OR (95% CI)
Urine culture positive
3.11 (1.47 - 6.55)
2.17 (1.07 - 4.39)
0.24 (0.03 - 1.90)
ID consult or managementc
Skin soft tissue infection
Time to effective therapy
1.06 (2.6) days
1.8 (3.7) days
Length of stay after culture
10.1 (11.3) days
13.5 (14.1) days
Multiple Cox regression analysis of factors predictive of death among patients with S. aureus bacteremia with one contemporaneous urine culture obtained
Hazard Ratio (95% CI)
Urine culture positive for S. aureus
Bladder catheter present
Univariate analysis of risk factors for bacteriuria among patients with S. aureus bacteremia
Urine culture positive
Urine culture negative
OR (95% CI)
2.17 (1.00 - 4.77)
3.78 (1.22 - 11.73)
0.41 (0.17 - 1.00)
Skin soft tissue infection
1.87 (0.89 - 3.94)
5.92 (2.41 - 14.54)
Multiple Cox regression analysis of risk factors for bacteriuria in patients with S. aureus bacteremia
OR (95% CI)
In our case-control study, patients who had S. aureus bacteremia and a positive urine culture for S. aureus had significantly higher mortality than did bacteremic patients with a negative urine culture. This was true even after adjustment for multiple covariates including use of bladder catheters, presence or absence of lower urinary tract symptoms, or recent urological surgeries, which are known risk factors for a positive urine culture, significant factor in the multivariable analysis such as presence of line infection or phlebitis or presence of comorbidities as measured by the Charlson score [2, 11]. The conclusion that S. aureus bacteriuria patients have worse prognosis compared to patients without S. aureus bacteriuria among S. aureus bacteremia patients may be useful to clinicians when deciding whether to monitor a patient on the ward or the ICU and when explaining the prognosis of the illness to the patient and his family.
S. aureus is a common pathogen in the community and in hospitals. S aureus causes significant mortality and morbidity but is an infrequent cause of urinary tract infection . In patients with S. aureus bacteremia, a positive urine culture is typically attributed to ascending infection or to hematogenous spread. Predictors of a positive urine culture for S. aureus include presence of indwelling bladder catheters, urinary tract obstruction, instrumentation, or surgery [27, 28]. We hypothesized that S. aureus appears in the urine in bacteremic patients because of higher burden of organisms and thus may portend worse outcome.
A recent study examining the relationship of bacteriuria with mortality found a two-fold increased risk of death in patients with concomitant S. aureus bacteriuria and bacteremia . The current study adds to published literature by including significant numbers of methicillin-resistant isolates and by using statistical methods appropriate for varying time at risk. The association of bacteriuria with increased risk of mortality even after adjustment for comorbidities was similar in both studies, suggesting that bacteriuria provides useful prognostic information in the setting of S. aureus bacteremia. In addition, based on our findings, it appears that this is an effect independent of methicillin-resistance and that secular trends in incidence of MRSA are unlikely to alter this association.
There are several limitations to our study. The patients in the study were cared for at a single center and it may be difficult to generalize our findings to other hospitals. The Cook County Bureau of Health Services is the largest provider of indigent care in Cook County and has seen large increases in rates of community-associated S. aureus infection, and our estimates likely represent robust measures of risk for mortality. A second limitation is that this was a retrospective study and depended on documentation in the chart, which may have resulted in misclassification errors in assessing comorbidities and other covariates. Non-differential misclassification tends to bias towards the null, which suggests that our estimates of risk are likely conservative. In addition, electronic data were used in this study and were prospectively collected at the time of care; these data were not subject to problems inherent in reviews of written documentation Finally, in our study, we had an overall mortality rate of 12% which was at the lower end of published mortality rates--12-46% [3–13, 27, 29, 30]--in this setting, suggesting that our cohort may have had fewer comorbidities and lower attributable mortality that in other published data. This may be a result of the significant burden of CA-MRSA in our patient population.
In patients with S. aureus bacteremia, a positive urine culture for S. aureus may be used to identify patients at higher risk of death. Patients who have S. aureus bacteremia and more comorbidities are at increased risk for higher mortality rate.
We thank Izumi Chihara for assistance with statistical analysis.
Financial support: This study was funded in part by the CDC Chicago Epi-Center Grant Number 5U01CI000327.
- Lee BK, Crossley K, Gerding DN: The association between Staphylococcus aureus bacteremia and bacteriuria. Am J Med. 1978, 65 (2): 303-306. 10.1016/0002-9343(78)90824-0.View ArticlePubMedGoogle Scholar
- Lesens O, Methlin C, Hansmann Y, Remy V, Martinot M, Bergin C, Meyer P, Christmann D: Role of comorbidity in mortality related to Staphylococcus aureus bacteremia: a prospective study using the Charlson weighted index of comorbidity. Infect Control Hosp Epidemiol. 2003, 24 (12): 890-896. 10.1086/502156.View ArticlePubMedGoogle Scholar
- Shurland S, Zhan M, Bradham DD, Roghmann MC: Comparison of mortality risk associated with bacteremia due to methicillin-resistant and methicillin-susceptible Staphylococcus aureus. Infect Control Hosp Epidemiol. 2007, 28 (3): 273-279. 10.1086/512627.View ArticlePubMedGoogle Scholar
- Kaech C, Elzi L, Sendi P, Frei R, Laifer G, Bassetti S, Fluckiger U: Course and outcome of Staphylococcus aureus bacteraemia: a retrospective analysis of 308 episodes in a Swiss tertiary-care centre. Clin Microbiol Infect. 2006, 12 (4): 345-352. 10.1111/j.1469-0691.2005.01359.x.View ArticlePubMedGoogle Scholar
- Blot SI, Vandewoude KH, Hoste EA, Colardyn FA: Outcome and attributable mortality in critically Ill patients with bacteremia involving methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Arch Intern Med. 2002, 162 (19): 2229-2235. 10.1001/archinte.162.19.2229.View ArticlePubMedGoogle Scholar
- Soriano A, Martinez JA, Mensa J, Marco F, Almela M, Moreno-Martinez A, Sanchez F, Munoz I, Jimenez de Anta MT, Soriano E: Pathogenic significance of methicillin resistance for patients with Staphylococcus aureus bacteremia. Clin Infect Dis. 2000, 30 (2): 368-373. 10.1086/313650.View ArticlePubMedGoogle Scholar
- Jensen AG, Wachmann CH, Poulsen KB, Espersen F, Scheibel J, Skinhoj P, Frimodt-Moller N: Risk factors for hospital-acquired Staphylococcus aureus bacteremia. Arch Intern Med. 1999, 159 (13): 1437-1444. 10.1001/archinte.159.13.1437.View ArticlePubMedGoogle Scholar
- Mylotte JM, Tayara A: Staphylococcus aureus bacteremia: predictors of 30-day mortality in a large cohort. Clin Infect Dis. 2000, 31 (5): 1170-1174. 10.1086/317421.View ArticlePubMedGoogle Scholar
- McClelland RS, Fowler VG, Sanders LL, Gottlieb G, Kong LK, Sexton DJ, Schmader K, Lanclos KD, Corey R: Staphylococcus aureus bacteremia among elderly vs younger adult patients: comparison of clinical features and mortality. Arch Intern Med. 1999, 159 (11): 1244-1247. 10.1001/archinte.159.11.1244.View ArticlePubMedGoogle Scholar
- Laupland KB, Church DL, Mucenski M, Sutherland LR, Davies HD: Population-based study of the epidemiology of and the risk factors for invasive Staphylococcus aureus infections. J Infect Dis. 2003, 187 (9): 1452-1459. 10.1086/374621.View ArticlePubMedGoogle Scholar
- Bader MS: Staphylococcus aureus bacteremia in older adults: predictors of 7-day mortality and infection with a methicillin-resistant strain. Infect Control Hosp Epidemiol. 2006, 27 (11): 1219-1225. 10.1086/507924.View ArticlePubMedGoogle Scholar
- Gomez J, Garcia-Vazquez E, Banos R, Canteras M, Ruiz J, Banos V, Herrero JA, Valdes M: Predictors of mortality in patients with methicillin-resistant Staphylococcus aureus (MRSA) bacteraemia: the role of empiric antibiotic therapy. Eur J Clin Microbiol Infect Dis. 2007, 26 (4): 239-245. 10.1007/s10096-007-0272-x.View ArticlePubMedGoogle Scholar
- Romero-Vivas J, Rubio M, Fernandez C, Picazo JJ: Mortality associated with nosocomial bacteremia due to methicillin-resistant Staphylococcus aureus. Clin Infect Dis. 1995, 21 (6): 1417-1423.View ArticlePubMedGoogle Scholar
- Desachy A, Lina G, Vignon P, Hashemzadeh A, Denis F, Etienne J, Francois B, Ploy MC: Role of superantigenic strains in the prognosis of community-acquired methicillin-susceptible Staphylococcus aureus bacteraemia. Clin Microbiol Infect. 2007, 13 (11): 1131-1133. 10.1111/j.1469-0691.2007.01810.x.View ArticlePubMedGoogle Scholar
- Fowler VG, Sanders LL, Sexton DJ, Kong L, Marr KA, Gopal AK, Gottlieb G, McClelland RS, Corey GR: Outcome of Staphylococcus aureus bacteremia according to compliance with recommendations of infectious diseases specialists: experience with 244 patients. Clin Infect Dis. 1998, 27 (3): 478-486. 10.1086/514686.View ArticlePubMedGoogle Scholar
- Jensen AG, Wachmann CH, Espersen F, Scheibel J, Skinhoj P, Frimodt-Moller N: Treatment and outcome of Staphylococcus aureus bacteremia: a prospective study of 278 cases. Arch Intern Med. 2002, 162 (1): 25-32. 10.1001/archinte.162.1.25.View ArticlePubMedGoogle Scholar
- Huggan PJ, Murdoch DR, Gallagher K, Chambers ST: Concomitant Staphylococcus aureus bacteriuria is associated with poor clinical outcome in adults with S. aureus bacteraemia. J Hosp Infect. 2008, 69 (4): 345-349. 10.1016/j.jhin.2008.04.027.View ArticlePubMedGoogle Scholar
- Ganz T: Defensins in the urinary tract and other tissues. J Infect Dis. 2001, 183 (Suppl 1): S41-42. 10.1086/318838.View ArticlePubMedGoogle Scholar
- Svanborg C, Bergsten G, Fischer H, Godaly G, Gustafsson M, Karpman D, Lundstedt AC, Ragnarsdottir B, Svensson M, Wullt B: Uropathogenic Escherichia coli as a model of host-parasite interaction. Curr Opin Microbiol. 2006, 9 (1): 33-39. 10.1016/j.mib.2005.12.012.View ArticlePubMedGoogle Scholar
- De NS: Experimental pyelonephritis in the rabbit produced by staphylococcal infection. J Pathol Bacteriol. 1950, 62 (3): 429-436. 10.1002/path.1700620315.View ArticleGoogle Scholar
- National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2003, issued August 2003. Am J Infect Control. 2003, 31 (8): 481-498. 10.1016/j.ajic.2003.09.002.
- Fridkin SK, Hageman JC, Morrison M, Sanza LT, Como-Sabetti K, Jernigan JA, Harriman K, Harrison LH, Lynfield R, Farley MM: Methicillin-resistant Staphylococcus aureus disease in three communities. N Engl J Med. 2005, 352 (14): 1436-1444. 10.1056/NEJMoa043252.View ArticlePubMedGoogle Scholar
- Hota B, Ellenbogen C, Hayden MK, Aroutcheva A, Rice TW, Weinstein RA: Community-associated methicillin-resistant Staphylococcus aureus skin and soft tissue infections at a public hospital: do public housing and incarceration amplify transmission?. Arch Intern Med. 2007, 167 (10): 1026-1033. 10.1001/archinte.167.10.1026.View ArticlePubMedGoogle Scholar
- King MD, Humphrey BJ, Wang YF, Kourbatova EV, Ray SM, Blumberg HM: Emergence of community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone as the predominant cause of skin and soft-tissue infections. Ann Intern Med. 2006, 144 (5): 309-317.View ArticlePubMedGoogle Scholar
- Deyo RA, Cherkin DC, Ciol MA: Adapting a clinical comorbidity index for use with ICD-9-CM administrative databases. J Clin Epidemiol. 1992, 45 (6): 613-619. 10.1016/0895-4356(92)90133-8.View ArticlePubMedGoogle Scholar
- Naimi TS, LeDell KH, Como-Sabetti K, Borchardt SM, Boxrud DJ, Etienne J, Johnson SK, Vandenesch F, Fridkin S, O'Boyle C, et al: Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA. 2003, 290 (22): 2976-2984. 10.1001/jama.290.22.2976.View ArticlePubMedGoogle Scholar
- Demuth PJ, Gerding DN, Crossley K: Staphylococcus aureus bacteriuria. Arch Intern Med. 1979, 139 (1): 78-80. 10.1001/archinte.139.1.78.View ArticlePubMedGoogle Scholar
- Arpi M, Renneberg J: The clinical significance of Staphylococcus aureus bacteriuria. J Urol. 1984, 132 (4): 697-700.PubMedGoogle Scholar
- Hawkins C, Huang J, Jin N, Noskin GA, Zembower TR, Bolon M: Persistent Staphylococcus aureus bacteremia: an analysis of risk factors and outcomes. Arch Intern Med. 2007, 167 (17): 1861-1867. 10.1001/archinte.167.17.1861.View ArticlePubMedGoogle Scholar
- Mylotte JM, McDermott C, Spooner JA: Prospective study of 114 consecutive episodes of Staphylococcus aureus bacteremia. Rev Infect Dis. 1987, 9 (5): 891-907.View ArticlePubMedGoogle Scholar
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