Fig. 3

Detection of ESBL and/or AmpC production using the Mast D68C

Disc A contained cefpodoxime (10 µg), Disc B contained cefpodoxime (10 µg) and an ESBL inhibitor, Disc C contained cefpodoxime (10 µg) and an AmpC inhibitor, Disc D contained cefpodoxime (10 µg) and both the AmpC and ESBL inhibitors

(a): An E. coli isolate showing ESBL production only: B - A and D - C ≥ 5 mm AND The difference between B & D and between A & C are < 4 mm

(b): A K. pneumoniae isolate showing AmpC production only: C - A and D - B are ≥ 5 mm AND The difference between A & B and between C & D are < 4 mm

(c): An E. coli isolate showing ESBL and AmpC co-production: D - C ≥ 5 mm AND The difference between A & B is < 4 mm

(d): A K. pneumoniae isolate showing negative result for both ESBL and AmpC production: A difference of ≤ 2 mm is present between all zones (A, B, C and D)