Table 1 ssDNA and gRNA sequences for detection of MTB DNA
From: A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system
component | ssDNA | gRNA |
|---|---|---|
IS6110-gRNA1 | CTATACAAGACCGAGCTGAT | UAAUUUCUACUAAGUGUAGAUAUCAGCUCGGUCUUGUAUAG |
IS6110-gRNA2 | GCGCGCTGGGTCGACTGGTT | UAAUUUCUACUAAGUGUAGAUAACCAGUCGACCCAGCGCGC |
IS1081-gRNA1 | GCCGGATGGAGCGCCTGGTC | UAAUUUCUACUAAGUGUAGAUGACCAGGCGCUCCAUCCGGC |
IS1081-gRNA2 | CGCTGCAGCAGCCAGTCCGG | UAAUUUCUACUAAGUGUAGAUCCGGACUGGCUGCUGCAGCG |