Table 2 Mutation distribution of rpoB, katG, inhA promoter and gyrA genes in the smear-positive sputum specimens
Drugs | Genes | Nucleotide change | Amino acid change | Number(%) | Number of phenotypically resistant strains |
|---|---|---|---|---|---|
rifampicin | rpoB a | G(1546) → T | Asp(516) → Tyr | 2(1.59) | 1 |
A(1547) → T | Asp(516) → Val | 1(1.59) | 1 | ||
A(1547) → T;A(1552) → Gb | Asp(516) → Val; Asn(518) → Asp | 1(1.59) | 1 | ||
AGA(1550–1552)deletionb | Frame shift | 1(1.59) | 1 | ||
C(1576) → T | His(526) → Tyr | 2(2.18) | 2 | ||
C(1576) → A | His(526) → Asn | 2(2.18) | 1 | ||
C(1576) → G | His(526) → Asp | 1(1.59) | 1 | ||
A(1577) → T | His(526) → Leu | 4(6.35) | 4 | ||
A(1577) → G | His(526) → Arg | 1(1.59) | 1 | ||
A(1577) → C | His(526) → Pro | 1(1.59) | 1 | ||
C(1592) → T | Ser(531) → Leu | 44(68.25) | 44 | ||
T(1598) → C | Leu(533) → Pro | 4(6.35) | 4 | ||
C(1542) → T;ATGGAC(1543–1548)deletionb | Frame shift | 1(1.59) | 1 | ||
isoniazid | katG | G(944) → C | Ser(315) → Thr | 40(95.24) | 40 |
G(944) → A | Ser(315) → Asn | 2(4.76) | 2 | ||
inhA promoter | T(−8) → C |  | 1(7.69) | 1 | |
C(−15) → T |  | 10(76.92) | 9 | ||
G(−17) → T |  | 1(7.69) | 1 | ||
C(−34) → Tc |  | 1(7.69) | 1 | ||
ofloxacin | gyrA | C(269) → T | Ala(90) → Val | 5(14.29) | 4 |
C(269) → G | Ala(90) → Gly | 2(5.72) | 1 | ||
T(271) → C | Ser(91) → Pro | 4(11.43) | 4 | ||
G(280) → T | Asp(94) → Tyr | 4(11.43) | 3 | ||
G(280) → A | Asp(94) → Asn | 5(14.29) | 4 | ||
A(281) → G | Asp(94) → Gly | 12(34.29) | 12 | ||
A(281) → C | Asp(94) → Ala | 3(8.57) | 3 |