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Table 3 Comparison of five markers in eggs deposited in liver and colon tissue from mice infected with S. japonicum post treatment

From: Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues

Staining

method

Marker

Positive rate (positive / total eggs; %) at different days post treatment with PZQ

Eggs in hepatic tissue

Eggs in colon tissue

30d

90d

180d

30d

90d

180d

 

Viable morphology

2.7*

(91/3369)

0.00*

(0/2713)

0.00

(0/1548)

2.8*

(126/4497)

0.00

(0/2320)

0.00

(0/1587)

NBT/

BCIP

ALP

83.52

(304/364)

34.88

(75/215)

5.83

(6/103)

75.35

(217/288)

0.00

(0/187)

0.00

(0/ 149)

von

Kossa

CalSâ–¼

78.57

(176/224)

15.22*

(21/138)

2.97

(3/101)

68.75

(143/208)

8.45

(12/142)

2.94

(3/102)

DAB

AOS

13.97**

(96/687)

0.00**

(0/323)

0.00

(0/312)

19.90**

(83/417)

0.00

(0/220)

0.00

(0/126)

MTT

SDHG

11.41**

(67/587)

5.70**

(29/509)

0.00

(0/456)

7.16**

(38 /531)

3.08

(9/292)

0.00

(0/266)

  1. ALP (alkaline phosphatase), CalS (calcified substance), AOS (antioxidase) and SDHG (succinic dehydrogenase). â–¼: Data ares non-calcified number of eggs. Compared with ALP: * P < 0.05; ** P < 0.01. All the data were collected from examining 18 blocks of liver or intestinal tissue samples from each of six mice