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Table 4 Discordant result of the UMD SelectNA and MicroSeq ID methods

From: Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples

Patient ID

Sample type

Indication

UMD SelectNA result

MicroSeq ID result

Other findings

Conclusion

6

Tissue

Infected hip prosthesis

S. dysgalactiae equisimilis

S. dysgalactiae equisimilis found with 16S in 3 other samples from the patient

R

9

Tissue

Mycotic aneurism

S. pneumoniae

Cocci found in blood culture bottle and positive antibody reaction for pneumococci

R

10

Tissue

Spinal implant infection

Staphylococcus lundunensis/hominis

Staphylococcus hominis cultured from tissue sample

R

11

Tissue

Infected knee prosthesis

S. aureus/simiae

S. aureus cultured from tissue

R

Tissue

S. aureus/simiae

R

12

Tissue

Infected hip prosthesis

Staphylococcus epidermidis/caprae/capitis

S. epidermidis cultured in 3 out of 5 biopsies

R

R

Tissue

S. epidermidis/caprae/capitis

13

Tissue

Cerebral abscess

Toxoplasma gondii (parasite)

 

R

14

Tissue

Infected hip prosthesis

Bacillus sp. & Thiotrix sp.

Two other tissue samples negative with the UMD and routine 16S/28S

A

15

Fluid from pacemaker electrode

Pericarditis

Kocuria rhizophila

Two other samples negative with UMD and routine 16S/28S

A

16

Implantation material

Spondylodiscitis

Hymenobacter arizonensis

Other implant material sample negative with UMD and routine 16S/28S

A

17

Tissue

Spondylodiscitis

Cloacibacterium normanense & Bergeyella sp. H1890

Fluid from back negative with UMD and routine 16S/28S

A

18

Lymph node

Lymphoma

S. epidermidis

 

A