Fig. 1

Screening of small-molecule inhibitors against HSV-1 induced necroptosis. a L929 cells were pretreated with compounds (10μM) from a library of 1280 chemicals for 1 h and subsequently infected with HSV-1 at a multiplicity of infection (MOI) value of 2 for 20h. Cell viability was assessed by measuring ATP levels. b The chemical structure of PHA767491. c L929 cells were pretreated with the indicated concentrations of PHA767491 for 1h and then treated with PBS or HSV-1 (MOI = 2) for 20 h and cell viability was measured. d L929 cells were pretreated with DMSO or PHA767491 for 1h and then infected with GFP-labeled HSV-1 for 16h. The fluorescence intensity was analyzed via inverted fluorescence microscopy