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Table 1 RPA primers and probe designed in this study

From: Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China

Name

Sequence

S. japonicum RPA FP

CCAAGTCTCAGTGAAGTTGTGAAGGCTAT

S. japonicum RPA RP

GTTAGTGTTCGAGACCAGTCAGATGGGATT

S. japonicum RPA P

CTTAAAGCGAGGGAGAGCGGCAGGACCAGA

(dT-FAM)G(THF)A(dT-BHQ1)TGACCCCTGAGATAT[3’-block]

  1. FP forward primer, RP reverse primers, P probe, dT-FAM thymidine nucleotide carrying fluorescein, THF tetrahydrofuran spacer, dT-BHQ1 thymidine nucleotide carrying black hole quencher
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BMC Infectious Diseases

ISSN: 1471-2334

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