Mechanisms of first-line antimicrobial resistance in multi-drug and extensively drug resistant strains of Mycobacterium tuberculosis in KwaZulu-Natal, South Africa

Table 1 Primer sequences and annealing temperatures used for PCR and sequencing

Gene	Primer	Nucleotide Sequence (5′ → 3′)	Annealing Temp (°C)	Associated Drug Resistance	Ref
rpoB	rpoB F	TGTTGGACATCTACCGCAAG	54 °C	Rifampicin	*
rpoB	rpoB R	CGAGACGTCCATGTAGTCCA	54 °C	Rifampicin	*
inhA	inhA F	CTACATCGACACCGATATGAC	55 °C	Isoniazid	[26]
inhA	inhA R	GACCGTCATCCAGTTGTAG	55 °C	Isoniazid	[26]
katG	katG F	GGTCGACATTCGCGAGACGTT	57 °C	Isoniazid	[27]
katG	katG R	TTGTTCCTGCGACGCATCGTG	57 °C	Isoniazid	[27]
pncA	pncA F	GCTGGTCATGTTCGCGATCG	59 °C	Pyrazinamide	[28]
pncA	pncA R	GCTTTGCGGCGAGCGCTCCA	59 °C	Pyrazinamide	[28]
embB	embB F	AAGCTGGCGCACCTTCAC	55 °C	Ethambutol	*
embB	embB R	ATAGCGCGGTGATCAAAAA	55 °C	Ethambutol	*

ISSN: 1471-2334