Figure 5
From: Cytometric detection of antigen-specific IFN-γ/IL-2 secreting cells in the diagnosis of tuberculosis
Secretion of IL-17 and IL-10 by PBMC stimulated with M. tuberculosis antigens. PBMC of controls (black bars, n = 6) or patients with TB (white bars, n = 14) were activated with SEA, PPD or ESAT-6/CFP-10 or left un-stimulated (None). After 72 hours supernatants were collected and examined for A) IL-17 and B) IL-10 content by ELISA. Detection limit of the assays is 15 pg/ml. Histograms represent the median value of cytokine secretion after each stimulus expressed in pg/ml.