| pg/ml†
|
---|
| | Patient cells††
|
---|
Cytokine | Healthy controls |
Before MTX
|
After MTX
|
---|
IFN-γ | 0-10 | 13 | <10 |
IL-1β | 0-10 | 1 100 | 2 |
IL-6 | 0-35 | 9 482 | 864 |
IL-8 | 150-2000 | 1 4795 | 1094 |
IL-10 | 0-10 | 425 | 4 |
TNF-α | 0-60 | 92 | <10 |
-
† Cytokine levels in supernatants from cells incubated 48 h in medium alone and quantified using FlowCytomix Human Th1/Th2 Kit as recommended (Bender Medsystems, Vienna, Austria, http://www.bendermedsystems.com).
-
†† No cytokine was detected in serum. IFN-α, IL-2, IL-4, IL-5 and IL-12 were not detected in these samples.