Probe | Melting peak | Mean Tm | SD | N | Tm Acceptance Criteria |
---|---|---|---|---|---|
136A2 | A136 | 62.0°C | 0.49 | 2228 | 60.0°C – 64.0°C |
136A2 | V136 | 53.2°C | 0.36 | 214 | 51.2°C – 55.2°C |
136A2 | T 136 | 56.9°C | 0.15 | 8 | 55.2°C – 58.9°C |
136A2 | AT 137 | 59.5°C | 0.19 | 7 | 59.0°C – 60.0°C |
154H2 | H154 | 56.6°C | 0.51 | 300 | 54.6°C – 58.7°C |
154H2 | R154 | 49.9°C | 0.29 | 2171 | 47.9°C – 51.9°C |
171R6 | R171 | 55.4°C | 0.39 | 1524 | 53.4°C – 57.4°C |
171R6 | Q171 | 49.8°C | 0.31 | 1392 | 48.4°C – 51.8°C |
171R6 | QE 175 | 49.7°C | 0.21 | 12 | 48.4°C – 51.8°C |
171R6 | L 168 Q | 47.9°C | 0.19 | 5 | 46.9°C – 48.4°C |
171R6 | H171 | 44.8°C | 0.27 | 168 | 42.8°C – 46.8°C |
171R6 | K 171 | 41.0°C | 0.15 | 8 | 39.0°C – 42.8°C |
141C3 | F141 | 53.9°C | 0.19 | 39 | 51.9°C – 55.9°C |
141C3 | L141 | 45.0°C | 0.14 | 6 | 43.0°C – 47.0°C |