Skip to main content

Table 1 Antibacterial properties of animal venoms tested against Burkholderia pseudomallei at 0.1 mg/mL concentration.

From: In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei

Common name

Scientific name

Phospholipase A2 enzymatic activity

Yield of protein (mg/mL)

Micro-organisms

  

Total activity (μmoles/min)

Specific activity (μmoles/min/mg protein)

 

B. pseudomallei (KHW)

B. pseudomallei (TES)

Elapidae

      

Death adder

Acanthophis augtra

3.74

74.5 ± 0.5

0.1

-

-

Common death adder

Acanthophis antarcticus

190

487.5 ± 0.96

0.78

16.6 ± 0.43

14.3 ± 0.34

Northern death adder

Acanthophis praelongus

241.2

1416 ± 3.84

0.34

8.90 ± 0.23

8.47 ± 0.21

Desert death adder

Acanthophis pyrrhus

138

1150 ± 0.98

0.24

14.7 ± 0.23

16.5 ± 0.32

Hector

Androctonus australis

22.2

85.2 ± 0.27

0.52

-

8.13 ± 0.14

Malayan krait

Bungarus candidus

34.9

166.3 ± 0.56

0.42

-

-

Sea snake

Hydrophis cyanocinctus

3.3

40.5 ± 0.56

0.16

-

-

Indian cobra

Naja naja naja

293.4

1333 ± 1.7

0.44

12.2 ± 0.16

10.2 ± 0.09

Kreft's tiger snake

Notechis aterater

41.96

183.1 ± 0.59

0.46

-

-

Spitting cobra

Naja sumatrana

406.5

903.5 ± 0.6

0.90

-

-

Cobra

Naja kaouthia

228.4

1904 ± 3.8

0.24

8.17 ± 0.15

-

King brown snake

Pseudechis australis

434.5

3949 ± 3.2

0.22

27.7 ± 0.13

29.8 ± 0.105

Speckled brown snake

Pseudechis guttata

308.5

791 ± 2.0

0.78

25.4 ± 0.19

26.8 ± 0.109

Red-bellied black snake

Pseudechis porphyriacus

726.7

3303 ± 1.7

0.44

16.8 ± 0.15

14.2 ± 0.17

Collett's snake

Pseudechis colletti

15.7

111.8 ± 0.89

0.28

-

-

-

Pseudonaja inframaggula

1995

5945 ± 26.6

0.66

7.7 ± 0.16

8.32 ± 0.11

Western brown snake

Pseudonaja nuchalis

162.8

361.8 ± 0.6

0.90

8.2 ± 0.08

9.30 ± 0.11

Eastern brown snake

Pseudonaja textilis

416.8

832.3 ± 0.6

1

14.2 ± 0.09

8.10 ± 0.14

Dugite

Pseudechis affinis

218.7

376.8 ± 0.9

1.16

-

-

Tiger keelback

Rhabdophis tigrinus

36.3

259.1 ± 0.42

0.28

-

-

The coastal taipan

Oxyuranus scutellatus

1275.7

6075 ± 0.98

0.63

-

-

Viperidae

      

Pallas

Agkistrodon halys

86.5

157.4 ± 0.20

1.1

20.4 ± 0.14

26.4 ± 0.08

Puff adder

Bitis arietans

124.1

248.2 ± 0.27

0.54

18.2 ± 0.16

16.2 ± 0.17

West African gaboon viper

Bitis gabonica rhinoceros

126.5

452.4 ± 0.57

0.56

16.0 ± 0.19

14.5 ± 0.26

Common lancehead snake

Bothrops atrox (L-amino acid oxidase)

3.8

6.36 ± 0.06

1.2

24.4 ± 0.19

26.2 ± 0.19

South American rattlesnake

Crotalus adamanteus (L-amino acid oxidase)

34.2

201.3 ± 0.21

0.34

25.4 ± 0.208

23.4 ± 0.18

South American rattlesnake

Crotalus adamanteus

236.4

619.4 ± 0.46

1.56

30.0 ± 0.23

28.3 ± 0.23

Saw-scaled viper

Echis carinatus

53.4

106.5 ± 0.49

1.4

8.0 ± 0.14

7.5 ± 0.15

Indian Russell's viper

Daboia russelli russelli

392.8

785.2 ± 0.40

1.36

29.9 ± 0.12

28.6 ± 0.16

Burmese Russell's viper

Daboia russelli siamensis

262.4

524.4 ± 0.44

1.24

16.2 ± 0.15

15.6 ± 0.18

Wagler's pit viper

Trimeresurus wagleri

4.6

38.2 ± 0.26

0.24

15.1 ± 0.14

16.3 ± 0.14

Apiidae

      

Honeybee venom

Apis mellifera

3.7

20.5 ± 0.1

0.36

7.22 ± 0.23

12.3 ± 0.21

Scorpionidae

      

Black scorpion

Androctonus crasicuda

3.6

69.5 ± 0.3

0.104

-

-

Scorpion

Buthotus hottenlota

4.4

10.4 ± 0.2

0.86

-

-

Scorpion

Buthotus hottenota hottenota

5.1

38.8 ± 0.1

0.26

-

-

Chinese red scorpion

Buthus martensii Karsch.

4.6

90.4 ± 0.2

0.102

-

-

Ceftazidime (CF)

30 μg/disc

-

-

-

28.9 ± 0.56

31.7 ± 0.78

Chloramphenicol (C)

30 μg/disc

-

-

-

28.5 ± 0.37

30.0 ± 0.12

Streptomycin (S)

10 μg/disc

-

-

-

27.3 ± 0.25

26.4 ± 0.29

  1. Values of Phospholipase A2 enzymatic activity (μmoles/min/mg protein) are presented as mean ± S.D. (n = 10) of ten replicates.
  2. *The values given as mean ± S.D. (n = 5) represent a venom inhibition zone in mm, including the 7 mm diameter of the disc, after 24 h incubation. The bacterial inoculum per plate contained 3.2 × 108 colony forming units (cfu/mL) which were spread onto the TS agar surface with sterile cotton swap. Sterile paper discs (7 mm diameter) were placed onto the TS agar surface and 20 μL of venom solution (0.1 mg/mL) added. Micro-organisms: B.p (KHW)-Burkholderia pseudomallei (KHW) and B.p (TES) Burkholderia pseudomallei (TES), Control (0); No activity (-), Drug controls: Chloramphenicol (30 μg/disc); Ceftazidime (30 μg/disc); Streptomycin (10 μg/disc).