Sample concentration: | Subtype A | Subtype B | Subtype C | Subtype D |
---|
Log dilution | Mean Cpa
| Mean Cp | Mean Cp | Mean Cp |
-2 | 26,16 | 22,90 | 24,61 | 26,13 |
-3 | 29,50 | 26,02 | 28,10 | 29,27 |
-4 | 32,82 | 29,75 | 31,79 | 32,86 |
-5 | 36,72 | 32,98 | 34,83 | 36,72 |
Slopeb
| -3,50 | -3,37 | -3,43 | -3,54 |
R2c
| 0,99 | 0,99 | 0,99 | 0,99 |
- (a) Mean of crossing point values calculated using two replicates of each point in 3 runs.
- (b) The LightCycler Software calculates the slope of the curve by plotting crossing points against the logarithm of concentration for each point of a 10-fold dilution series. A slope value of -3.33 indicates the maximal PCR efficiency.
- (c) The LightCycler Software also calculates the coefficient of correlation R2.