Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2

BMC Infectious Diseases

Table 2 Addition of the Δ30 mutation to rDEN-2 decreases replication in SCID-HuH-7 mice by 10-fold.

	Replication in SCID-HuH-7 mice^a
Virus	No. of mice	Mean virus titer (log₁₀PFU/ml ± SE)
DEN-2 Tonga/74	6	5.9 ± 0.3
rDEN-2	7	5.9 ± 0.2
rDEN2Δ30	9	4.9 ± 0.2

^a Groups of HuH-7-SCID mice were inoculated directly into the tumor with 10⁴ PFU virus. Serum was collected on day 7 and tittered in Vero cells.

ISSN: 1471-2334