From: Association of circulating Chlamydia pneumoniaeDNA with cardiovascular disease: a systematic review
Reference | Country | Cases | Prevalence (%) | Controls | Prevalence (%) | Blood sampling | DNA Extraction | PCRa target |
---|---|---|---|---|---|---|---|---|
Berger, 2000 [21] | Germany | Vascular surgery | 12 of 60 (20.0) | Not stated | 7 of 51 (13.7) | 4 mL EDTA blood | Phenol-chloroform | 16S rDNA |
Boman, 1998 [22] | Sweden | Coronary Angiography | 60 of 101 (59.4) | Blood donors | 24 of 52 (46.1) | 10 mL EDTAb blood | Phenol-chloroform | Nested MOMPc |
Da Costa, 2000 (abstract) | Germany | Coronary Angioplasty and antibiotics | 14 of 233 (6.0) | Blood donors | 5 of 200 (2.5) | Not stated | QIAamp or phenol-chloroform | Not stated |
Freidank, 2000 [23] | Germany | Carotid atherosclerosis | 10 of 60 (16.7) | Medical students, patients | 0 of 109 (0.0) | 10 mL whole blood | QIAamp | Nested pstI cloned fragment |
Kaul, 2000 [24] | United States | Coronary artery disease | 13 of 28 (46.4) | Blood donors | 5 of 19 (26.3) | 20–30 mL EDTA, CD3+ cell selection | SDS-proteinase K | Nested MOMP HSPd-60 |
Maraha, 2001 [25] | Netherlands | Aortic aneurysm | 18 of 88 (20.5) | Healthy controls | 8 of 88 (9.1) | EDTA blood | QIAamp | 16S rDNA |
Sessa, 2000 [26] | Italy | Unstable angina, myocardial infarction | 24 of 93 (25.8) | Healthy controls | 2 of 42 (4.8) | EDTA blood | Phenol-chloroform | Hemi-nested pstI cloned fragment |
Smieja, 2001 [27] | Canada | Coronary Angiography or angioplasty | 22 of 187 (11.8) | Normal angiogram | 2 of 21 (9.5) | 8 mL BD CPTc tube | QIAamp | Nested MOMP |
Wong, 1999 [28] | United Kingdom | Coronary Angiography | 79 of 913 (8.7) | Normal angiogram | 21 of 292 (7.2) | 4–5 mL EDTA blood | Phenol-chloroform | Nested MOMP |