Figure 2
Standard curves in vitro RNA Figure 2 a-d. Relationship of time-to-positivity (TTP) to HHV-8 mRNA copy number. The number of mRNA molecules present in the reaction is indicated on the x axis and the ttp value in indicated on the y axis. For the data obtained with 50, 102 to 107 molecules, the values of TTP are the mean of five replicates of independent experiments. For the U1A the range of RNA input was 103 to 108. Error bars indicate the standard deviation for the values of ttp. The solid line was obtained by linear regression analysis of the data from 50 to 107 molecules, and the dotted lines indicate the 95% confidence intervals for the regression. Inserts a-d: Amplification plot of a 10-fold dilution serial dilution of in vitro RNA for ORF 73, vGCR, vBcl-2 and vIL-6. The amount of input RNA 1 * 107, 1 * 106, 1 * 105, 1 * 104, 1 * 103, 1 * 102, 50 and 0 molecules. Insert e: Amplification plot of a 10-fold dilution serial dilution of U1A in vitro RNA, the amount of input RNA 1 * 108, 1 * 107, 1 * 106, 1 * 105, 1 * 104, 1 * 103 and 0 molecules.