Figure 3

Standard curve obtained with the G6PD L. infantum qPCR test. The standard curve was generated by plotting the Ct against the input target quantity (in this case, serial dilutions of Leishmania DNA respresenting 10⁶ to 10¹ parasites). Ct represents the fractional cycle number reflecting a positive PCR result differentiated from the background noise. Slope -3.206, PCR efficacy 105%, R² = 0.971.