Figure 5
From: Serum biomarkers predictive of cure in Chagas disease patients after nifurtimox treatment
MALDI identification of human fibronectin. MALDI MS-MS data confirms the identification of the 28.9 kDa biomarker to be a fragment of human FN1. (A) MS Reflector Positive spectrum obtained from the in-gel trypsin digestion of the ~30 kDa band cut out from fraction 2 shows the precursor peaks generated by trypsin. (B) ProteinPilot MS-MS data shows that 14 peptides were sequenced and matched to human FN1 with more than 95% confidence. (C) MS-MS sequence coverage. Peptides sequenced with more than 95% confidence (green) covered 48.3% of the amino-terminal domain of the FN1 human protein sequence.