An adjuvanted inactivated murine cytomegalovirus (MCMV) vaccine induces potent and long-term protective immunity against a lethal challenge with virulent MCMV

Table 3 Long-term protection in mice immunized with inactivated vaccine with or without adjuvant ^a

Immunogen	Dosage (μg)	ELISA titer (2ⁿ) of IgG antibody^b(6 months after the last immunization)	Protection against SGV challenge
Immunogen	Dosage (μg)		Survival mice/total mice
Adjuvant-free	4	8.0 ± 0.8	3/14
+Chitosan		9.5 ± 1.0^d	6/14^c
+Alum		11.7 ± 0.5^d	12/14^c,d
+MF59		12.3 ± 0.6^d	13/14^c,d
PBS	-	-	1/15

^aMice were immunized by i.p. injection twice, 3 weeks apart, with 4 μg of the inactivated vaccine with or without an adjuvant (MF59, alum, or chitosan). Mice were challenged with a lethal dose of SG-MCMV 6 months after the last immunization. Serum samples from the immunized mice were collected 2 days prior to challenge. The survival of mice 3 weeks after the challenge was measured.
^bValues represent means ± SD of each group.
^cSignificant difference (p < 0.05), compared with control.
^dSignificant difference (p < 0.05), compared with the corresponding adjuvant-free subjects.

ISSN: 1471-2334