Table 1 Primer pairs used in the PCR/ESI-MS MRSA typing and characterization assay
Rowa | Primer pair code | Target(s) | Accession number and coordinates | Forward and reverse primer sequences | Reference |
|---|---|---|---|---|---|
A | BCT879 | mecA | Y14051 | 5′-TCAGGTACTGCTATCCACCCTCAA-3′ | [14] |
4507..4581 | 5′-TGGATAGACGTCATATGAAGGTGTGCT-3′ | ||||
BCT2157 | pta | NC_003923 | 5′-TCTTGTTTATGCTGGTAAAGCAGATGG-3′ | [13] | |
629121..629229 | 5′-TGGTACACCTGGTTTCGTTTTGATGATTTGTA-3′ | ||||
B | BCT2249 | tufB | NC_002758.2 | 5′-TGAACGTGGTCAAATCAAAGTTGGTGAAGA-3′ | [14] |
615038..616222 | 5′-TGTCACCAGCTTCAGCGTAGTCTAATAA-3′ | ||||
BCT2163 | yqi | NC_003923 | 5′-TGAATTGCTGCTATGAAAGGTGGCTT-3′ | [13] | |
379057..379199 | 5′-TCGCCAGCTAGCACGATGTCATTTTC-3′ | ||||
C | BCT3016 | mupA | X75439 | 5′-TAGATAATTGGGCTCTTTCTCGCTTAAAC-3′ | [14] |
2482..2573 | 5′-TAATCTGGCTGCGGAAGTGAAAT-3′ | ||||
BCT2161 | tpi | NC_003923 | 5′-TCCCACGAAACAGATGAAGAAATTAACAAAAAAG-3′ | [13] | |
830671..830799 | 5′-TGGTACAACATCGTTAGCTTTACCACTTTCACG-3′ | ||||
D | BCT3106 | TSST1 | NC_002758.2 | 5′-TCGTCATCAGCTAACTCAAATACATGGA-3′ | This work |
519..620 | 5′-TCACTTTGATATGTGGATCCGTCATTCA-3′ | ||||
BCT3025 | arcC | NC_003923 | 5′-TGAATAGTGATAGAACTGTAGGCACAATCGT-3′ | [13] | |
2724791..2724920 | 5′-TGCGCTAATTCTTCAACTTCTTCTTTCGT-3′ | ||||
E | BCT2095 | PVL genes, lukE-lukD | NC_003923 | 5′-TGAGCTGCATCAACTGTATTGGATAG-3′ | [14] |
1529595..1531285 | 5′-TGGAAAACTCATGAAATTAAAGTGAAAGGA-3′ | ||||
BCT2149 | aroE | NC_003923 | 5′-TGGGGCTTTAAATATTCCAATTGAAGATTTTCA-3′ | [13] | |
1674546..1674697 | 5′-TACCTGCATTAATCGCTTGTTCATCAA-3′ | ||||
F | BCT2256 | nuc | NC_002758.2 | 5′-TACAAAGGTCAACCAATGACATTCAGACTA-3′ | [14] |
894288..894974 | 5′-TAAATGCACTTGCTTCAGGGCCATAT-3′ | ||||
BCT2166 | yqi | NC_003923 | 5′-TAGCTGGCGGTATGGAGAATATGTCT-3′ | [13] | |
379190..379311 | 5′-TCCATCTGTTAAACCATCATATACCATGCTATC-3′ | ||||
G | BCT4022 | PVL / lukD | NC_003923 | 5′-TGGTCAACAAAGCTATATCAGTGAAGTAGAAC-3′ | This work |
1529595..1531285 | 5′-TGGATCATGTCCAGACATTTCACCTAATG-3′ | ||||
BCT2156 | gmk | NC_003923 | 5′-TCACCTCCAAGTTTAGATCACTTGAGAGA-3′ | [13] | |
1191206..1191337 | 5′-TGGGACGTAATCGTATAAATTCATCATTTC-3′ | ||||
H | BCT4024 | ileS | NC_002758.2 | 5′-TCAGTTGCTACAAGAGGAGTATCACCT-3′ | This work |
1247700..1250453 | 5′-TACTCATCTTCTTACCTTCACCGTCCATA-3′ | ||||
BCT2150 | aroE | NC_003923 | 5′-TGATGGCAAGTGGATAGGGTATAATACAG-3′ | [13] | |
1674392..1674523′ | 5′-TAAGCAATACCTTTACTTGCACCACCTG-3′ |