Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia

Table 2 Dengue detection by RT-PCR, NS1, and IgM ELISAs on samples from Indonesia

Parameter	RT-PCR	NS1	NS1 sensitivity (%)	IgM^a	IgM sensitivity (%)	p-value^b
City (island)
Denpasar (Bali)	13	10/13	76.9	5/13	38.5	0.131
Jakarta (Java)	9	8/9	88.9	4/9	44.4	0.221
Jayapura (Papua)	8	3/8	37.5	7/8	87.5	0.134
Kendari (Sulawesi)	3	1/3	33.3	2/3	66.7	1.000
Medan (Sumatra)	6	5/6	83.3	4/6	66.7	1.000
Samarinda (Borneo)	24	1/24	4.2	17/24	70.8	<0.001
Semarang (Java)	29	22/29	75.9	27/29	93.1	0.182
Surabaya (Java)	96	56/96	58.3	74/93	79.6	0.001
Total Indonesia	188	106/188	56.4	140/188	73.7	<0.001
Serotype
DENV-1	86	56/86	67.4	74/84	88.1	0.002
DENV-2	19	13/19	68.4	12/19	63.2	1.000
DENV-3	32	22/32	68.8	17/32	53.1	0.332
DENV-4	42	8/42	19.0	31/42	73.8	<0.001
Mix-infection	9	5/9	55.6	6/8	75.0	0.617
Infection status ^a
Primary	71	48/71	67.6	40/71	56.3	0.201
Secondary	114	55/114	48.2	100/114	87.7	<0.001
Severity ^c
DF	59	30/59	50.8	34/58	58.6	0.486
DHF	120	72/120	60.0	97/118	82.2	<0.001
DSS	9	4/9	44.4	9/9	100	0.074

ISSN: 1471-2334