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Figure 2 | BMC Infectious Diseases

Figure 2

From: Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia

Figure 2

Comparison of ITS1/PCR results using template DNA prepared by: A) The phenol-based method. B) The sodium hydroxide-based method. C) The potassium acetate method. DNA was prepared from two Whatman 3MM filter paper discs with dried blood containing Leishmania donovani promastigotes. 1 ml human blood was spiked with different numbers of promastigotes: lane 1 = 106, lane2 = 105, lane 3 = 104, lane 4 = 103, lane 5 = 102, lane 6 = 10, lane 8 = 0.

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BMC Infectious Diseases

ISSN: 1471-2334

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