Dynamics of pneumococcal nasopharyngeal carriage in healthy children attending a day care center in northern Spain. influence of detection techniques on the results

Table 2 Streptococcus pneumoniae serotype distribution in nasopharyngeal samples of carriers collected on three distinct dates

	Time of sampling			Single or multiple carriage per sample				Total samples with this serotype	Serotyping method			Number of children colonized with this serotype
Serotype^a	Winter 2004	Spring 2005	Winter 2005	Single	With another one	With another two	With another three		Quellung^b	Pneumotest Latex^c	Multiplex PCR
4			1	1				1	1	1	1	1
5	1					1		1	0	0	1	1
6A		2			2			2	2	2	2	2
6B	13	2		2	9	4		15	15	14	15	14
6C	1						1	1	1	1	1	1
9V		2		1	1			2	2	2	2	2
9N		1				1		1	1	1	1	1
10A	3	8	1	8	3	1		12	11	12	12	11
10B	1			1				1	1	1	1	1
11A	12	2		2	8	3	1	14	11	11	14	13
13	1	1				2		2	2	2	2	2
14		6	6	8	3	1		12	6	6	12	12
15B	8	10	3	14	5	2		21	20	20	21	20
16F	14	9		8	11	3	1	23	17	17	23	21
19A	9	12	20	30	9	2		41	41	41	41	36
19F	2	4		2	3	1		6	6	6	6	5
20	6	9	1	4	9	3		16	6	6	16	16
21	7	6		9	3	1		13	13	13	13	13
22F	2	1			2	1		3	1	1	3	3
23B	6	3	1	7		2	1	10	10	10	10	9
35F	1	1	6	5	2	1		8	8	8	8	7
Total pneumococci serotyped	87	79	39	102	70	29	4	205	175	175	205	191
Non typeable isolates	7	4	2	13				13	13	13	13	11
Total pneumococci detected	94	83	41	115	70	29	4	218			218

^a Serotype: in bold, serotypes included in the seven-valent pneumococcal conjugate vaccine
^b Quellung: serotype 6C-specific antiserum, factor 6 d, was not available at the moment of the study and serotype 6C was verified by using factor antiserum 6b
^c Pneumotest-Latex kit: the kit used did not allow the identification at the serotype level, being identifications done at the serogroup level

ISSN: 1471-2334