Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis

BMC Infectious Diseases

Table 1 Number of articles reporting subject and laboratory characteristics stratified by exposure route

	Vaccination	Natural Exposure	Total
Geographic location of study population
Asia	2	15	17
North America	5	11	15*
South America	0	1	1
Africa	0	0	0
Australia	1	0	1
Europe	0	2	2
Not reported	1	1	1*
Neutralization percentage
50 %	5	18	23
70 %	0	3	3
80 %	2	2	3*
90 %	0	1	1
Not reported	1	2	3
Cell line
BHK	0	9	9
LLC-MK₂	5	11	16
Vero	3	4	6*
Not reported	0	2	2
Virus concentration (plaque-forming units [pfu] or focus-forming units [ffu] per microliter [uL] or milliliter [mL])
10-20 pfu / 10 uL	0	1	1
10-20 pfu / 50 uL	0	2	2
15-25 pfu / 25 uL	0	2	2
20-30 pfu / 12.5 uL	0	2	2
40-80 pfu / 100 uL	0	1	1
50 pfu / 150 uL	3	4	7
10⁷ pfu / mL	2	0	2
25-50 pfu	0	1	1
50 pfu	0	1	1
320 pfu	0	1	1
100 ffu / 90 uL	0	1	1
800 ffu	1	1	1*
Not reported	2	8	10

* indicates articles do not sum in row as Vasilakis et al. [18] reported titers from vaccination and natural exposure.

ISSN: 1471-2334