Table 2 Intra- and inter-assay evaluation of repeatability with clinical samples from patients experiencing residual viraemia
From: HIV-1 low-level viraemia assessed with 3 commercial real-time PCR assays show high variability
% of samples where at least one replicate is not detected | % of samples where only one triplicate is detected | % of samples where at least one replicate was higher than 50 cop/mL | % of samples where at least one replicate was higher than 100 cop/mL | % of samples where at least one replicate was higher than 200 cop/mL | |
|---|---|---|---|---|---|
Intra-assay Siemens (N=40) | 52.5 | 32.5 | 2.5 | 2.5 | 0.0 |
Intra-assay Abbott (N=40) | 57.5 | 20.0 | 15.0 | 5.0 | 0.0 |
Intra-assay Roche (N=40) | 55.0 | 20.0 | 12.5 | 2.5 | 0.0 |
Inter-assay (N=61) | 52.5 | 13.1 | 18.0 | 4.9 | 3.3 |