Figure 2
Chromosomal location of tet (L) genes in S. suis isolates. (A1, B1) PFGE patterns of SmaI digested genomic DNA of four S. suis serotype 2 strains, (A2, B2): Southern blot hybridization of SmaI digested genomic DNA of four strains with 698 bp- tet(L) probe. (A) Lane 1: S. suis BM308; lane 2: S. suis BM407; (B) lane 1: S. suis EN031; lane 2: S. suis EN241. (A, B) Lane 3: 698 bp-tet(L) PCR amplicons, which was added to the well of PFGE gel one hour prior to completion of the PFGE running programme. Molecular weights of marker bands, consisting of XbaI digested genomic DNA of Salmonella serotype Braenderup strain (H9812), spanning the region containing the positive signals of tet(L), are indicated.