Figure 3
Stability of influenza RNA at 4 C in different matrices at different spiking concentrations. 3.55 × 106, 3.55 × 105, or 3.55 × 104 EID50 of H1N1 virus in 10 μl of PBS diluted as stated in Materials and Methods was mixed with 130 μl of PBS, plasma, or buffy coats for different periods of time indicated. Real-time RT-PCR was performed using TaqMan, the results were shown as Ct value in RT-PCR. (A). Comparison of RNA recovery from different concentrations of virus stored in buffy coats. (B). Comparison of RNA recovery from 10 μl of 3.55 × 106 EID50 of H1N1 virus stored in PBS, plasma, or buffy coats. (C). Comparison of RNA recovery from 10 μl of 3.55 × 105 EID50 of H1N1 virus stored in PBS, plasma, or buffy coats.