Molecular detection and speciation of pathogenic Leptospiraspp. in blood from patients with culture-negative leptospirosis

Table 2 Nucleotide distance based on the 443-nucleotide fragment and almost full-length fragment of the rrs gene

Nucleotide distance based on the 443-nucleotide *rrs*
Average distance (%)
	1	2	3	4	5	6	7	8	9	10	11	12	13
1 L. interrogans
2 L. kirschneri	0.27
3 L. borgpetesenii	1.81	1.58
4 L. noguchii	1.13	0.90	1.58
5 L. santarosai	2.28	2.05	1.37	1.83
6 L. weilii	1.81	1.62	0.68	2.00	2.05
7 L. alexanderi	1.58	1.35	0.90	1.58	1.83	1.09
8 L. alstonii	0.90	0.68	1.35	0.90	1.83	1.77	1.13
9 L. kmetyi	1.58	1.35	1.58	1.35	2.50	2.00	1.81	0.68
10 L. fainei	4.14	3.87	4.36	4.03	4.61	4.78	4.59	3.91	3.69
11 L. inadai	4.51	4.25	4.74	4.63	4.31	5.38	4.74	4.29	4.51	1.20
12 L. broomii	4.74	4.47	4.51	4.63	4.54	5.16	4.97	4.51	4.29	0.98	0.23
13 L. wolffii	5.42	5.17	5.42	5.42	5.66	6.06	5.87	5.42	5.19	2.56	2.71	2.48
14 L. licerasiae	5.87	5.62	5.87	5.87	6.12	6.51	6.32	5.87	5.64	3.01	3.16	2.93	0.45
Nucleotide distance based on almost whole length fragment of *rrs* gene
Average distance (%)
	1	2	3	4	5	6	7	8	9	10	11	12	13
1 L. interrogans
2 L. kirschneri	0.13
3 L. borgpetesenii	0.77	0.69
4 L. noguchii	0.64	0.57	0.83
5 L. santarosai	1.11	1.03	0.81	0.91
6 L. weilii	1.00	0.92	0.48	1.00	1.05
7 L. alexanderi	0.99	0.89	0.63	1.06	1.23	0.92
8 L. alstonii	0.89	0.83	1.08	0.81	1.42	1.28	1.24
9 L. kmetyi	0.95	0.89	0.99	0.80	1.35	1.21	1.27	0.53
10 L. fainei	4.93	4.81	4.86	4.86	5.09	5.22	5.24	5.11	4.62
11 L. inadai	4.84	4.71	4.79	4.90	4.86	5.27	5.09	5.04	4.77	0.61
12 L. broomii	5.06	4.94	4.86	5.01	5.01	5.27	5.32	5.27	4.77	0.43	0.23
13 L. wolffii	4.62	4.54	4.58	4.72	4.80	4.98	4.86	4.85	4.55	2.42	2.58	2.35
14 L. licerasiae	4.32	4.24	4.30	4.27	4.42	4.58	4.48	4.47	4.09	2.65	2.73	2.58	1.36

ISSN: 1471-2334