Figure 6

Production of reactive nitrogen and reactive oxygen intermediates is similar in Cd36 ^+/+ and Cd36 ^-/- macrophages. A. Nitric oxide production was measured using the Griess reaction in culture supernatant. In response to stimulation with BCG, nitric oxide production is TLR2 (*p < 0.0001) and IRAK-4 (*p < 0.0001) dependent, but independent of TLR4 (p = 0.16) and CD36 (p = 0.83). B. Similar nitric oxide production by Cd36 ^+/+ and Cd36 ^-/- macrophages in response to live and heat-killed BCG and M. marinum, as well as specific toll-like receptor ligands (Pam₃CSK₄ (PAM), a TLR2 ligand, and lipopolysaccharide (LPS); p > 0.05 for all comparisons Cd36 ^+/+ vs Cd36 ^-/-). C and D. Similar kinetics of nitric oxide production over 36 hours in response to both BCG (C) and M. marinum (D). E and F. Similar oxidative burst in response to phorbol myristate acetate (PMA), and no significant response to BCG above baseline among Cd36 ^+/+ and Cd36 ^-/- macrophages.