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Table 1 Primer sequences. Primer sequences used for the detection of norovirus. Primers for both genotypes target the ORF1/ORF2 overlap of the viral genome.

From: The Prevalence of Norovirus in returning international travelers with diarrhea

Genogroup

Primer

Sequence

Location*

G I

NV 192

5'- gC (CT) Atg TTC CgC Tgg Atg C - 3'

5282 - 5300

G I

NV 193

5'- CgT CCT Tag ACg CCA TCA TCA - 3'

5379 - 5359

G I

TM8

5'- FAM - Tgg ACA gg(Ag) gAT CgC (Ag)AT

5321 - 5345

 

Probe

CTC CTg C - 3' - TMR

 

G II

NV 107a

5'- AgC CAA TgT TCA gAT ggA Tg - 3'

5007 - 5026

G II

NV 117

5'- TCg Acg CCA TCT TCA TTC AC-3'

5100 - 5081

G II

TM3

5'- FAM - Tgg gAg ggC gAT CgC AAT CTg gC -

5048 - 5070

 

Probe

3' - TMR

 
  1. *Genome location of primers and probes are based for GI on the sequence of Norwalk/68/US [GenBank: M87661] and for GII on the sequence of Lordsdale/93/UK [GenBank: X86557];