Figure 1

Experimental design for in vivo selection of drug-resistant HSV. This cartoon depicts the methodology used to select drug-resistant virus. Both ear pinnae of each mouse were infected with HSV. There were five replicate mice per treatment group (treated suboptimally with 0.2 mg/ml FCV or VCV, or with placebo) and isolates from seven serial in vivo passages examined. The resulting virus preparation from the pooled ears of an individual mouse (sample number 1, passage number 1) were used to inoculate both ear pinnae of one mouse for the subsequent serial passage (sample number 1, passage number 2).