Laboratory-acquired infections of Salmonella enterica serotype Typhi in South Africa: phenotypic and genotypic analysis of isolates

Background Workers in clinical microbiology laboratories are exposed to a variety of pathogenic microorganisms. Salmonella species is among the most commonly reported bacterial causes of laboratory-acquired infections. We report on three cases of laboratory-acquired Salmonella enterica serotype Typhi (Salmonella Typhi) infection which occurred over the period 2012 to 2016 in South Africa. Methods Laboratory investigation included phenotypic and genotypic characterization of isolates. Phenotypic analysis included standard microbiological identification techniques, serotyping and antimicrobial susceptibility testing. Genotypic analysis included the molecular subtyping methodologies of pulsed-field gel electrophoresis analysis, multilocus sequence typing and whole-genome sequencing (WGS); with WGS data analysis including phylogenetic analysis based upon comparison of single nucleotide polymorphism profiles of isolates. Results All cases of laboratory-acquired infection were most likely the result of lapses in good laboratory practice and laboratory safety. The following critical issues were highlighted. There was misdiagnosis and misreporting of Salmonella Typhi as nontyphoidal Salmonella by a diagnostic laboratory, with associated public health implications. We highlight issues concerning the importance of accurate fluoroquinolone susceptibility testing and interpretation of results according to updated guidelines. We describe potential shortcomings of a single disk susceptibility screening test for fluoroquinolone susceptibility and suggest that confirmatory minimum inhibitory concentration testing should always be performed in cases of invasive Salmonella infections. These antimicrobial susceptibility testing issues resulted in inappropriate ciprofloxacin therapy which may have been responsible for failure in clearance of pathogen from patients. Salmonella Typhi capsular polysaccharide vaccine was not protective in one case, possibly secondarily to a faulty vaccine. Conclusions Molecular subtyping of isolates proved effective to investigate the genetic relatedness of isolates. Molecular subtyping data interpreted together with epidemiological data allowed us to pinpoint the most likely sources for our cases of laboratory-acquired infection.


Background
Workers in clinical microbiology laboratories are exposed to a variety of pathogenic microorganisms [1,2]. A review of published literature in 2009, reported that bacteria account for the greatest number of reports of laboratoryacquired infections; Shigella species, Brucella species, Salmonella species, Mycobacterium tuberculosis and Neisseria meningitidis were the most commonly reported bacterial causes of laboratory-acquired infections [1].
There have been very few reports of laboratoryacquired infections involving Salmonella enterica serotype Typhi (Salmonella Typhi), however many may go unreported. A PubMed literature search on 1 February 2017, using the key words 'Salmonella Typhi laboratory acquired infection' found 12 publications (English language) reporting laboratory-acquired Salmonella Typhi infections [3][4][5][6][7][8][9][10][11][12][13][14]. These 12 papers were published over the period 1961 to 1997, so over the last 10 years, to the best of our knowledge, there are no recent publications of laboratory-acquired Salmonella Typhi infections. However, more recently, there have been reports of nontyphoidal Salmonella (NTS) laboratory-acquired infections. In 2016, a Salmonella enterica serotype Typhimurium (Salmonella Typhimurium) laboratory-acquired infection was reported from Canada by Alexander and coworkers [15]; while in 2014 and 2012, the Centers for Disease Control and Prevention (CDC) reported on Salmonella Typhimurium outbreaks in the USA of which the source of the outbreaks were traced to university teaching microbiology laboratories [16,17] In 2015, Barker and coworkers reported on a Salmonella Enteritidis laboratory-acquired infection [18].
In our present study, we report on three cases of laboratory-acquired Salmonella Typhi infection which occurred over the period 2012 to 2016 in South Africa. We demonstrate the usefulness of various molecular subtyping techniques to investigate the source of the infections.

Case reports
Three cases of laboratory-acquired Salmonella Typhi infection occurred over the period 2012 to 2016 in South Africa. We describe these as follows.

Case one
In September 2012, a laboratory technologist working at Laboratory-A presented to her clinician with signs of severe malaise, fever and mild diarrhoea. The technologist had six years of working experience in a clinical microbiology laboratory. Blood cultures were collected for laboratory testing. The diagnostic laboratory identified Salmonella using the MALDI Biotyper and the isolate failed to agglutinate in Salmonella Typhi-specific antisera. Subsequently, the isolate was reported to the clinician as NTS, susceptible to fluoroquinolones. The patient was treated with seven days of oral ciprofloxacin. The isolate was referred to the Centre for Enteric Diseases (CED) at the National Institute for Communicable Diseases (NICD), for confirmation of identification. The CED confirmed Salmonella Typhi with intermediate resistance to fluoroquinolones [ciprofloxacin minimum inhibitory concentration (MIC), 0.25 μg/ml] and susceptibility to azithromycin (MIC, 4 μg/ml). The patient, though recovered, was still excreting Salmonella Typhi in her stool and was then treated with a combination of azithromycin and fluoroquinolone for 14 days. Subsequent stools tested negative for Salmonella Typhi. The patient, being a laboratory technologist with frequent exposure to Salmonella Typhi, had received Salmonella Typhi vaccination nine months earlier as part of an occupational health program. On investigation, it was found that this vaccine was part of a batch recalled by the manufacturer, because of potentially low (below specification) antigen content [19] .

Case two
In February 2016, a trainee clinical pathology resident working on the stool bench at a clinical microbiology laboratory (Laboratory-B), presented to a physician with fever, nausea and vomiting. The patient was admitted to hospital and discharged three days later. Salmonella Typhi was cultured from microbiological cultures of blood taken on admission. The isolate was referred to the CED for confirmation of identification. The CED confirmed Salmonella Typhi with susceptibility to fluoroquinolones (ciprofloxacin MIC, 0.008 μg/ml) and to azithromycin (MIC, 8 μg/ml). The patient responded well to levofloxacin therapy. Further investigation revealed that the patient had recently serotyped a clinical isolate of Salmonella Typhi in the laboratory and had not worn gloves at the time. The patient had one year of working experience in a clinical microbiology laboratory and had not been vaccinated against Salmonella Typhi.

Case three
In October 2016, a laboratory technologist working in a clinical microbiology laboratory (Laboratory-C) started complaining of headaches at work. His colleagues offered him an over the counter analgesic but his symptoms persisted. He reported to a hospital emergency room with symptoms of diarrhoea, worsening headaches, temperatures of 39°C and severe malaise; he was admitted into hospital. Blood chemistry revealed raised liver enzymes and a C-reactive protein (CRP) reading of 90 mg/L. Blood cultures were collected for laboratory testing; Salmonella Typhi was cultured. The diagnostic laboratory determined that the isolate was susceptible to ceftriaxone and the fluoroquinolones (Kirby-Bauer disk susceptibility testing method -pefloxacin disk (5-μg) screening zone size of 25 mm). The patient was commenced on intravenous ciprofloxacin by the treating physician. He received seven days of intravenous ciprofloxacin followed up by a seven-day course of oral ciprofloxacin on discharge. The isolate was referred to the CED for confirmation of identification. The CED confirmed the Salmonella Typhi identification and determined that the isolate was intermediately-resistant to fluoroquinolones (ciprofloxacin MIC, 0.25 μg/ml) and susceptible to azithromycin (MIC, 4 μg/ml). Salmonella Typhi with the same MICs was isolated from subsequent stool samples, collected 16 days and 19 days after the blood culture sample tested positive. The patient was contacted by the treating private physician and readmitted to hospital where he received five days of intravenous azithromycin. On discharge he was prescribed combination therapy comprising of oral ciprofloxacin and azithromycin for one month. Subsequent stools tested negative for Salmonella Typhi. The technologist had 28 months working experience in a clinical microbiology laboratory and had no history of vaccination against typhoid fever. Epidemiological investigation determined that the technologist had been exposed to Salmonella Typhi while processing a culture of Salmonella Typhi.

Identification of typhoid fever cases and public health response
Typhoid fever is a notifiable disease in South Africa. Based on laboratory diagnosis, typhoid fever is relatively uncommon in South Africa. Laboratory networks report the isolation of Salmonella Typhi to the Outbreak Response Unit of the NICD, who in turn notify the district and provincial communicable diseases coordinators. Cases are investigated through a home visit, interviewed and a case investigation form is completed. Cases are later followed up and include further testing of stool samples. For persons who have had contact with cases, their symptoms are assessed and stool samples are also collected for testing. Details of all cases, including patient occupation are reported to the NICD and recorded in a database. Databases are reviewed to identify cases whose occupation includes laboratory work; data collection includes demographic details, clinical and treatment history and outcome data. For the currently described three cases: all patients came from relatively affluent and hygienic home circumstances, and there was no suggestion of family members being ill or other likely sources of infection; laboratory infection control procedures and laboratory safety policies were reviewed and refresher staff training was conducted; we contacted patients and obtained written informed consent to describe their case.

Referral of bacterial isolates to the CED
The CED is the national reference centre in South Africa for human infections due to enteric pathogens including: Salmonella species, Shigella species, Campylobacter species, diarrhoeagenic Escherichia coli, Vibrio cholerae and Listeria monocytogenes. Isolates from across South Africa are voluntarily submitted to the CED through national laboratory-based surveillance from >200 clinical microbiology laboratories across the country, in relation to potential outbreaks. The CED proceeds with phenotypic and genotypic characterization of isolates. If required, molecular subtyping of isolates is performed.
To determine if these cases of typhoid fever in laboratory-workers were acquired from the isolates to which they were exposed in the laboratory, we identified isolates of Salmonella Typhi that were submitted to the CED from the laboratory in which the patient worked during the two months prior to onset of illness and subjected these isolates and the patient's isolate to phenotypic and genotypic characterization, as described below.

Phenotypic characterization of bacteria
Bacteria were received on Dorset-Egg transport media [Diagnostic Media Products (DMP), National Health Laboratory Service, Johannesburg, South Africa] and subcultured onto 5% Blood Agar (DMP), to check for viability and purity. Cultures were identified using standard phenotypic microbiological identification and serotyping techniques, briefly described as follows. As required, bacterial colonies were identified using the VITEK-2 COMPACT 15 automated microbial identification system (bioMérieux, Marcy-l'Étoile, France). Serotyping was performed according to the White-Kauffmann-Le Minor Scheme. Antimicrobial susceptibility testing was performed using the VITEK-2 COMPACT 15 system (bioMérieux) and the Etest method (bioMérieux). Interpretation of antimicrobial susceptibility data was done in accordance with the Clinical and Laboratory Standards Institute (CLSI) [20].
PCR for the H58 haplotype of Salmonella Typhi PCR to determine whether Salmonella Typhi isolates belonged to the H58 haplotype were performed according to the methodology described by Murgia and coworkers [21].
Pulsed-field gel electrophoresis (PFGE) analysis of bacteria PFGE analysis of XbaI digested genomic DNA was performed using a Bio-Rad CHEF-DR III electrophoresis system (Bio-Rad Laboratories, Hercules, USA), following a PulseNet protocol [22]. PFGE patterns were analyzed using BioNumerics (version 6.5) Software (Applied Maths, Sint-Martens-Latem, Belgium) with dendrograms of the patterns created using the unweighted pair group method with arithmetic averages, with analysis of banding patterns incorporating the Dice-coefficient at an optimization setting of 1.5% and a position tolerance setting of 1.5%.

Whole-genome sequencing (WGS) analysis of bacteria
Genomic DNA was isolated from bacteria using the Qiagen QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). DNA libraries were prepared using a Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA), followed by a 2 × 300 paired-end sequencing runs with 100× coverage using Illumina MiSeq equipment. Raw data generated on the MiSeq was further analyzed using tools available in the CLC Genomics Workbench Software, version 8.5 (Qiagen). Using the 'Trim Sequences Tool' , sequence reads were trimmed to include quality trimming and ambiguity trimming, and length trimming to discard reads below a length of 50 bases. Trimmed reads were assembled using the 'De novo Assembly Tool'; the assembly algorithm works by using de Bruijn graphs to produce contiguous (contig) sequences (minimum contig length was set at 200 bases).

Multilocus sequence typing (MLST) of bacteria
Assembled genome data was analyzed using the 'multilocus sequence typing (MLST)' on-line analysis pipeline available at the Center for Genomic Epidemiology (CGE) of the Technical University of Denmark [23]. MLST produces sequence types (STs) based on sequence analysis of seven housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA and thrA), as described at the Salmonella MLST database [24].

Single nucleotide polymorphism (SNP) profiles and phylogenetic analysis of bacteria
Assembled genome data was analyzed using the 'CSI-Phylogeny 1.4' on-line analysis pipeline available at the CGE [25]. The CSIPhylogeny pipeline uses various publicly available programs and the analysis steps are briefly described as follows: assembled genome data is aligned against a reference genome and single nucleotide polymorphisms (SNPs) are called; SNPs are filtered and qualified; final qualified SNPs for each genome is concatenated to an alignment; phylogeny is then inferred based on a comparison of SNP alignments of strains. SNPs were called by alignment and referencing against a South African strain isolated in 2016 (reference number TCD981492). SNP alignments were analyzed with iTOL software [26] to generate phylogenetic maximum-likelihood trees.

Results
The results for molecular subtyping of isolates are as follows.
For case one, molecular subtyping using PFGE analysis showed that the PFGE pattern of the patient's isolate was indistinguishable (100% identical) to that of a PFGE pattern shown by a cluster of Salmonella Typhi isolates that the patient (laboratory technologist) had been working on in Laboratory-A (Fig. 1), suggesting that the source of the patient's infection was within this cluster of isolates that the patient had been working on. This 'cluster of isolates' was sourced from a region of South Africa located~1500 km from where the laboratory technologist lived and worked; also the laboratory technologist had no travel history or any history of contact with persons that lived/travelled to this region of South Africa. Therefore, this could not have been a case of community exposure to Salmonella Typhi. For case one, MLST presented a ST 1 subtype for the patient's isolate; the isolate was also PCR-positive for a marker associated with haplotype H58, a haplotype of Salmonella Typhi which is being reported with increasing frequency from many countries in Africa and Asia [27,28].
For case two, molecular subtyping of isolates included PFGE analysis, MLST and SNP profiling. We investigated all Salmonella Typhi isolates that the patient (resident) could have been exposed to in Laboratory-B. Compared to three possible isolates, PFGE analysis showed a PFGE pattern match (Fig. 2) to a single isolate (isolate A) from Laboratory-B. Phylogenetic analysis and SNP profiling showed that isolate A was related to case two; the isolates only differed by 41 SNPs (Fig. 2). Both isolates also presented the MLST ST 2 subtype and both were PCR-negative for the marker associated with haplotype H58. These data suggested that the source of the patient's infection was isolate A. The patient later confirmed that she had indeed worked with isolate A.
For case three, molecular subtyping of isolates included PFGE analysis, MLST and SNP profiling. We investigated all Salmonella Typhi isolates that the patient (laboratory technologist) could have been exposed to in Laboratory-C. Compared to two possible isolates (isolates B and C) that the patient could have been exposed to in Laboratory-C; PFGE analysis showed a PFGE pattern match (Fig. 3) to one of the isolates (isolate B). This was supported by MLST; case three presented ST 1 which matched the ST 1 of isolate B; isolate C presented ST 2 and so did not match the case. The case isolate and isolate B were both PCR-positive for the marker associated with haplotype H58. Phylogenetic analysis and SNP profiling confirmed that isolate B was highly related to case three with the isolates only differing by 6 SNPs (Fig. 3). These data suggested that the source of the patient's infection was isolate B. a Snapshot from a dendrogram of PFGE (XbaI digestion) patterns (b) Snapshot from a phylogenetic maximum-likelihood tree drawn using SNP alignments. Note: isolate 958151 was isolated from a specimen collected on 4 June 2016 (4 months after isolation of the case 2 isolate), so case 2 could not have been exposed to isolate 958151

Discussion
Case one highlight's issues concerning the misdiagnosis and misreporting of a Salmonella Typhi isolate as a NTS by a diagnostic laboratory; with associated public health implications, as typhoid fever is a notifiable disease in most countries including South Africa. Salmonella Typhi has a high potential to cause outbreaks of disease [29,30], therefore rapid diagnosis and reporting to the health authorities is vital to ensure appropriate investigation including follow up of the patient and tracing the patient's contacts, as well as to design interventions to avert any potential outbreak.
Cases one and three highlights issues concerning the importance of accurate fluoroquinolone susceptibility testing and interpretation of results according to updated guidelines [20]. For case one, the patient was treated initially with inappropriate therapy with ciprofloxacin due to the use of defunct interpretative criteria for fluoroquinolone resistance [31], which was later followed with appropriate therapy with azithromycin. For case three, the discrepancy between the pefloxacin screening result (25 mm or susceptible) and the ciprofloxacin MIC (0.25 μg/ml or intermediately-resistant) for the isolate highlights the potential shortcomings of a single disk susceptibility screening test for fluoroquinolone susceptibility and suggests that confirmatory MIC testing should always be performed in cases of invasive Salmonella infections. While appropriate antimicrobial therapy does not always result in clearance of Salmonella Typhi from the stool, in both cases one and three, treatment with ciprofloxacin to which the isolates were intermediately-resistant failed to clear the pathogen whilst subsequent therapy with azithromycin [32] was successful.
All cases highlight issues concerning Salmonella Typhi vaccination of laboratory workers who are at high risk of exposure to Salmonella Typhi. Neither of the two laboratory workers described in cases two or three had received a Salmonella Typhi vaccination, despite exposures to pure cultures. Case one had received vaccine based on Vi polysaccharide antigen, but no currently available vaccine against typhoid is 100% protective; vaccination decreases risk of infection but does not completely eliminate the risk of infection. Vaccine failures are reported [33,34]. For case one, vaccination was ineffective; vaccine failure may have occurred secondary to a faulty vaccine. In developed countries such as the USA, Canada, United Kingdom and Denmark; vaccination against Salmonella Typhi is offered to and encouraged for laboratory workers who are at high risk of exposure to Salmonella Typhi, such as those working in reference laboratories. In South Africa, this is certainly the policy and procedure at the CED at the NICD; however not all laboratory networks in South Africa provide occupationspecific vaccination. For reference laboratories in most other African countries, vaccination of laboratory workers against Salmonella Typhi is not routine practice. In 1980, a review of Salmonella Typhi laboratory-acquired infections reported that of 24 cases, the vast majority of patients (19 cases; 79.2%) had not been vaccinated; so lack of vaccination is a definite risk factor [8].
All our cases of laboratory-acquired infection were most likely the result of lapses in good laboratory practice (GLP) and laboratory safety. Case one had six years of working experience in a clinical microbiology laboratory, yet laboratory-infection still occurred. Lapses in laboratory safety do occur, even among laboratory workers with many years of experience working in a clinical  Fig. 3 Molecular subtyping of Salmonella Typhi isolates. Snapshots are shown from dendrograms and phylogenetic trees of all South African isolates. a Snapshot from a dendrogram of PFGE (XbaI digestion) patterns (b) Snapshot from a phylogenetic maximum-likelihood tree drawn using SNP alignments microbiology laboratory. A recent report in 2015 described an NTS infection involving a laboratory technician with 20 years of experience in a clinical microbiology laboratory [18]. Over recent years, the decreasing numbers of reports concerning Salmonella laboratory-acquired infections probably reflect the current increased awareness and practice of GLP and laboratory safety. Risky procedures such as mouthpipetting are now no longer practiced. Today, clinical microbiology laboratories in most countries enforce uniform safety policies nationwide, which would include: safety training; restricted laboratory access; use of biological safety cabinets; personal protective equipment (PPE) (gloves, masks, coats, etc.); hand-washing facilities with automatic-operating taps or elbow operating taps and automatic-operating paper towel dispensers; prohibiting eating, drinking and smoking in the laboratory; prohibiting cell phones in the laboratory; proper decontamination of laboratory benches before and after working with clinical specimens and bacterial cultures; etc. Laboratory accreditation to International Organization for Standardization (ISO) requirements ensures compliance and audit safety policies; diagnostic laboratories in South Africa are all accredited against the ISO 15189 standard [35]. With regards to use of biological safety cabinets, in South Africa (and most African countries), these are not mandatory for working with Salmonella Typhi. However, for some laboratories in Europe and the USA, it is mandatory to work with Salmonella Typhi within a biological safety cabinet (cabinets with a minimum of level-2 safety) or within a biosafety level-2 laboratory.
Molecular subtyping of bacterial isolates is an effective methodology to investigate the relatedness of bacterial isolates and to investigate the source of the infections. Molecular subtyping, including PFGE analysis [3,[15][16][17][18] and WGS [15], has previously been reported to investigate laboratory-acquired infections involving Salmonella species. In particular, Alexander and coworkers [15] used WGS to identify the source of a laboratory-acquired infection involving a laboratory technologist; interestingly, the technologist was not responsible for any testing conducted on the causative strain, so it was concluded that the infection was probably acquired while cleaning laboratory benches or discarding biohazardous waste.
For our current cases, all isolates were investigated with PFGE analysis. Although PFGE analysis is an older molecular subtyping methodology, it still provides valuable information and still remains the primary bacterial subtyping methodology employed by PulseNet International, a molecular subtyping network for foodborne and waterborne disease surveillance. PFGE analysis is limited in that it may not always provide sufficient resolution; for example, if you have a case isolate and you query a database of isolates to find a closest match by genetic relatedness, you may find that the case isolate will equally match (by PFGE pattern) a number of isolates. This was the situation for our case one, where the PFGE pattern for the case isolate was found to match a PFGE pattern represented by a cluster of isolates (Fig. 1), suggesting that the source of the laboratory worker's infection was within this cluster of isolates that the laboratory worker had handled. To pinpoint the best possible epidemiological match, PFGE data analyzed together with epidemiological data (dates, geographic locations, patient contact information, etc.) is helpful. This was the situation for cases two and three, whereby PFGE data interpreted together with epidemiological data allowed us to pinpoint the most likely source of the laboratory worker's infection; source isolate A for case two (Fig. 2) and source isolate B for case three (Fig. 3).
For any finer degree of resolution, alternative molecular subtyping methodologies must be employed. The ultimate methodology is WGS data analysis; this WGS methodology was used to investigate isolates for cases two and three, thereby providing a secondary analysis to the primary PFGE analysis. Our analysis of WGS data included a phylogenetic analysis based upon a comparison of SNP profiles. When the number of SNP differences is compared among isolates, the lowest number of SNP differences implies the closest genetic relationship. This allowed us to determine the exact genetic similarity between a case isolate and its probable source. For case two, the case isolate and the probable source isolate (isolate A) differed by only 41 SNPs and were located within close proximity on a phylogenetic tree (Fig. 2). For case three, the case isolate and the probable source isolate (isolate B) differed by only 6 SNPs and were located within close proximity on a phylogenetic tree (Fig. 3).
Lastly, a third molecular subtyping methodology, MLST, was employed to investigate our case isolates. MLST is generally more suitable for a long-term epidemiological comparison of isolates or a global comparison of isolates. However, in some cases of short-term epidemiological investigations (outbreak investigations), it can sometimes provide valuable supporting data. This was the situation for case three, whereby the laboratory worker had acquired his infection by exposure to two possible isolates; isolate B with MLST ST 1 or isolate C with MLST ST 2. The case three isolate presented MLST ST 1, providing evidence supporting isolate B as the cause of the infection.

Conclusions
Laboratory-acquired infection of Salmonella Typhi is uncommon, as the risk of infection is low if appropriate laboratory safety policies are in place and adhered to. Nonetheless, accidental infections still do occur and would most likely be the result of lapses in aseptic techniques. In these situations, molecular subtyping of bacterial isolates is very useful to investigate the source of infections. Availability of data and materials All material (including raw data) described in this publication is freely available. All datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions AMS: Conception and design of the study. Obtained ethics approval for the study. Analysis and interpretation of laboratory data. Preparation of all drafts of the manuscript. Collation of all edits/comments from coauthors. Continuous review and editing of drafts of the manuscript. Submission of the manuscript for publication. Corresponding author for the manuscript. SLS: Laboratory analysis of isolates. Interpretation of laboratory data. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. NT: Laboratory analysis of isolates. Interpretation of laboratory data. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. CB: Laboratory analysis of isolates. Interpretation of laboratory data. Obtained patient information and epidemiological data regarding isolates. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. VMM: Laboratory analysis of isolates. Interpretation of laboratory data. Obtained patient information and epidemiological data regarding isolates. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. CJ: Coordination of the public health response regarding isolation of Salmonella Typhi. Obtained patient information and epidemiological data regarding isolates. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. KMM: Coordination of the public health response regarding isolation of Salmonella Typhi. Obtained patient information and epidemiological data regarding isolates. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. AL: Laboratory analysis of isolates. Interpretation of laboratory data. Obtained patient information and epidemiological data regarding isolates. Review and critical assessment of the manuscript. Approved the final draft of the manuscript. KHK: Conception and design of the study. Interpretation of laboratory data. Review and critical assessment of the manuscript. Approved the final draft of the manuscript.
Ethics approval and consent to participate Ethical approval to publish this case study was obtained by Human Research Ethics Committees from the University of the Witwatersrand (certificate number M1703100) and from the University of Cape Town (reference number 306/2017).

Consent for publication
Informed consent for writing this publication was obtained from all patients -written informed consent was obtained. This publication has not documented any potentially-identifying information about the patients.