Skip to main content
Download PDF

High silent prevalence of human herpesvirus 1 (HSV-1) infection affecting the indigenous reservation of the municipality of Dourados, Central-West Brazil

BMC Infectious Diseases volume 24, Article number: 700 (2024) Cite this article

Abstract

Background

The indigenous population located in the central region of Brazil, is the second largest in terms of population size in the country. The Indigenous Reserve of Dourados has risk factors that increase the vulnerability of the indigenous population to infectious diseases, especially Human alphaherpesvirus (HSV-1), a neglected disease with high prevalence in priority populations in developing countries. The virus can also cause many more severe diseases, including widespread neonatal infections, herpetic keratitis, and herpes encephalitis, which can be fatal if left untreated. We estimated the prevalence of anti-HSV-1 antibodies and correlated it with the demographic and behavioral characteristics of the Indigenous population of the Jaguapirú and Bororó villages (Dourados, Mato Grosso do Sul (MS), Brazil).

Methods

Our approach was cross-sectional. From March 2017 to November 2018. Using anti-HSV-1 (Gg1) IgM and anti-HSV-1 (gG1) IgG Euroimmun and the detection and quantification of HSV-1 viral load in plasma samples, through real-time PCR. The maps were constructed using QGIS and the statistical analyses using R Studio software.

Results

A total of 1138 individuals (> 18 years old) were enrolled. The prevalence of anti-HSV-1 IgM and IgG were 20% and 97.5%, respectively. The prevalence of anti-HSV-1 antibodies for IgG was higher in both sexes. Anti-HSV-1 IgM antibodies were present in 17.1%, 21.2%, 12.5%, and 22% of the participants with urinary problems, genital wounds, genital warts, and urethral discharge, respectively. Real-time PCR was used for confirmatory testing; HSV-1 DNA was detected in 25.6% (54/211) of anti-HSV1 IgM-positive samples. Viral loads ranged from 5.99E + 02 to 3.36E + 13.

Conclusions

The seroprevalence of HSV-1 IgM and detection of HSV-1 DNA in the Indigenous population confirmed high silent prevalence. Furthermore, the seroprevalence of HSV-1 in the Indigenous population was higher than that reported in the general adult Brazilian population. Various socioeconomic factors, drug use, and health and sexual behaviors could contribute to the facilitation of HSV-1 transmission in the Indigenous population. Our results may help develop culturally appropriate intervention programs that eliminate health access barriers and improve the implementation of public health policies aimed at promoting information regarding the prevention, treatment, and control of HSV-1 infection in Brazilian Indigenous populations.

Peer Review reports

Introduction

The Indigenous “priority population” in the state of Mato Grosso do Sul (MS) is at increased risk of acquiring Human Alphaherpesvirus 1 (Human herpes virus 1 or HSV-1). According to the United Nations Declaration on the Rights of Indigenous Peoples, adopted through resolution A/RES/61/295 by the United Nations General Assembly, firmly affirms the equal entitlement of Indigenous communities to the utmost possible standard of physical and mental well-being. This includes their right to access quality healthcare services to safeguard their health needs and uphold their dignity [1]. The lack of information regarding HSV-1 prevalence is a priority when it comes to unique health behavior, awareness, and prevention. According to the 2010 census (conducted by the Brazilian Institute of Geography and Statistics (IBGE) and the National Indian Foundation (FUNAI), Brazil has an Indigenous population of approximately 896,917 individuals, with 324,834 living in urban areas and 572,083 living in rural areas; Currently about 1.3 million Indigenous people in Brazil. Indigenous people in Brazil include 305 ethnically diverse groups and 274 languages, spread over 12.5% of the Brazilian geographic area. The Indigenous population studied in Mato Grosso do Sul, located in the central region of Brazil [2] is the second largest in Brazil [2, 3].

Inhabiting the Jaguapirú and Bororó villages of the city of Dourados in Mato Grosso do Sul (MS), the largest ethnic groups are Guarani-Kaiowá and Terena, comprising approximately 18,000 people in an area of 3474.50 hectares [4, 5]. In addition, the Atikum, Kini Kinawa, Kadiwéu, Guató, Guarani-Nhandeva, and Ofaié ethnic groups inhabit the entire territory of MS [3,4,5]. The geolocation of Indigenous populations places them in close proximity to urban centers and international land borders. In turn, this increases interactions with non-Indigenous populations, and predisposes them to alcoholism and drug use. Additionally, Indigenous populations have low socioeconomic and educational levels related to cultural rituals, and risky sexual behaviors such as lack of condom use, unprotected sex, and a history of sexually transmitted infections (STIs), worsened by absent or limited access to health care and information. Further, several cultural rituals require the use of shared and inadequately disinfected sharp objects. These risk factors increase the vulnerability of the Indigenous population of the Dourados/MS area to infectious diseases, specifically HSV-1 [6,7,8,9,10,11]. The scarcity of specific epidemiological data on STIs from indigenous Brazilian communities greatly contributes to the higher morbidity and mortality rates of HSV-1 in these areas compared to the general population [11, 12].

The Herpesviridae family of enveloped DNA viruses including nine human herpesviruses (HSV) widely distributed worldwide. HSV-1 is a double-stranded DNA virus, transmitted through mucosal epithelial cells by breaks in the skin. It migrates to nervous tissue, where it persists in a latent state. In most cases it is localized to orofacial lesions in the trigeminal ganglia, and can reactivate at any time [13]. Most infections begin during childhood or youth via direct oral contact with secretions contaminated with viral particles. Lytic infection, in most cases, can cause mild illnesses such as cold sores and oral lesions (herpes labialis) [14, 15]. However, HSV-1 has been found to concomitantly infect the orofacial areas and the genital tract [13]. The virus is also capable of causing many more serious diseases, such as herpetic keratitis [16], herpes encephalitis, which can be fatal if left untreated [17, 18], and disseminated neonatal infections [19]. HSV-1 is being studied as a major etiological factor Alzheimer’s disease [18,19,20]. HSV-1 is also closely related to HSV-2, a human pathogen with an increased risk with human immunodeficiency virus-1 (HIV-1) infection [21, 22]. Reactivation can occur in response to various stimuli, such as immunosuppression, stress, or hormonal changes, which are symptomatic; however, transmission can also occur in an asymptomatic form [23,24,25,26]. Latent reactivation leads to asymptomatic or generalized episodes [26,27,28,29,30].

HSV-1 is a highly prevalent human pathogen. About 3,583.5 million of the global population aged 0–49 years have been infected orally with HSV-1 and its worldwide prevalence is approximately 63.6%. Genital HSV1 is prevalent in 5.2% of individuals aged 15–49 years worldwide. Simultaneous oral and genital infections are present in 66.6% of the population, of which 3,752.0 million people aged 0–49 years were infected globally. Moreover, 3.3% of individuals aged 15–49 years had genital HSV-1 and reported oral sex in the year prior (2016) [31, 32]. A study conducted in Latin America and the Caribbean revealed that the mean seroprevalence of HSV-1 was 57.2% among healthy adult populations and 90.9% among adult populations with comorbidities; in patients with clinically diagnosed genital ulcers, the average seroprevalence was 0.9%, and genital herpes, the average seroprevalence was 10.9% for HSV-1, due to other etiologies that generate genital ulcers [33]. The seroprevalence of HSV-1 antibodies in the general population of Brazil is 67.2%, accounting for a total of 1,090 individuals aged 1–40 years, and the overall seroprevalence among men and women is 66.2% and 68.2%, respectively and among Brazilian cities, the seroprevalence of HSV-1 was 73.3% in Manaus, 68.6% in Rio de Janeiro, 62.1% in Porto Alegre, and 47.0% in Fortaleza [34].

We aimed to estimate the prevalence and analyze factors associated with HSV-1 infection in the Indigenous population of the Dourados/MS Reserve using HSV-1 antibody from participants in the Jaguapirú and Bororó villages. Our research is useful for developing culturally appropriate programs that can facilitate access to public health services, eliminate stigmas regarding HSV-1 transmission and treatment, and support the implementation of public health policies to promote the prevention, treatment, intervention, and control of HSV-1 infections in the Brazilian Indigenous population.

Materials and methods

Ethical statement

All participants provided informed consent before participating in the study. The study adhered to the principles of the Declaration of Helsinki. Approval was received from the Ethics Committee of the University of Grande Dourados (UFGD-MS) in March 2017 (CA AE:62012616.3.0000.5160 (number 2.000.496)). This study was conducted with utmost professionalism and care, ensuring that all doubts and concerns were addressed promptly.

Sampling

The Dourados Indigenous Area Health Team (EMSI, Bororó, and Jaguapiró, 1 and 2) provided multidisciplinary expertise and support for this study. Our sampling team of doctors and nurses q1the use of sterile equipment and needles for venous blood draws.

Our approach was cross-sectional. From March 2017 to November 2018, a team of skilled health professionals consisting of medical doctors, physicians, biologists, and nurses, with the assistance of a proficient local interpreter, collected data and blood samples from the Indigenous population from two villages in Dourados, Mato Grosso do Sul (MS), Brazil. The research population was made up exclusively of indigenous adults aged 18 or over who were able to provide informed consent. Before participation, each individual voluntarily signed a comprehensive consent form and responded to a personalized socio-epidemiological survey, ensuring an optimal level of privacy and anonymity. To further validate our structured questionnaire, we sought input from Indigenous health professionals and an Indigenous health agent translated the questionnaire into the native language when needed.

The questionnaire-based interview aimed to capture a comprehensive profile of the participants, focusing on their risks and protective factors. We carefully selected information on risk behaviors for HIV and HBV, including sociodemographic information such as income, education status, and housing conditions, substance use history, medical history, and signs and symptoms of hepatitis B and C, HIV, and HSV infections. The study included consenting individuals aged 18 years who resided in the study area. Participants who failed to provide sufficient blood samples for anti-HSV-1 testing were excluded.

The study population was determined using data from the Special Indigenous Health District of Mato Grosso do Sul (SIHD/MS). According to SIHD/MS, there are 13,094 Indigenous individuals residing in Bororó and Jaguapirú within the Dourados/MS municipality, with 6291 over the age of 18. Factoring in a 20% loss due to refusal, the estimated eligible population for sampling was 3400, comprising both males and females, and individuals from the Guaraní-Kaiowá, Terena, Kadiwéu, and Guarani-Nhandeva ethnic groups. The selection criterion for the regression analysis to calculate the p-value, the POR (95% CI) above 0.20 was statistically calculated. The eligible sample size for our study included 295 individuals, comprising both men and women. We recruited 1,168 study participants over the age of 18 who agreed to take part in the survey; the remainder did not agree to take part in the survey, around four times more than the estimated sample size required. This represents 8.9% of the total population living in the Dourados Indigenous Reserve/MS.

Serological analysis

Anti-HSV-1 (gG1) IgM and anti-HSV-1 (gG1) IgG Euroimmun (Euroimmun, a PerkinElmer Company, Medizinische labordiagnostika AG-Germany: Anti-HSV-1 (gG1) ELISA (IgM) order no. EI 2531-9601-2 M LOTE: E180321AT and Anti-HSV-1 (gG1) ELISA (IgG) order no. El 2531-9601-2 G LOTE: E220906BL) ELISA (enzyme-linked immunosorbent assays) detected serological markers of HSV-1 infection. The sensitivity and specificity of both immunoassays were determined according to the manufacturer’s instructions using positive, calibrator, and negative controls. The test had a specificity of 100% and a sensitivity of 100%. The results were assessed using binary outcomes of positive and negative HSV-1 IgG and IgM. The ELISA technique ensured high antigen specificity, which minimized the incidence of false positives due to cross-reactions with anti-HSV-2. Therefore, it can be concluded that this assay is reliable and accurate as per the protocol.

Detection and quantification of HSV-1 viral load by qPCR UL 39

Detection and quantification of the viral load of HSV-1 in the plasma samples were performed using real-time PCR (qPCR). The TaqMan oligonucleotides and probes used in this study are listed. Single qPCR was performed in a reaction mix comprising 1 µL of 25x PCR enzyme (Life Technologies mix, California, USA), 1.25 µL of each oligonucleotide (1 µM), 1 µL probe (0.4 µM), 6 µL of 2x PCR Buffer (Life Technologies, California, USA) and 3 µL of DNA. Absolute quantification was performed using a previously described synthetic standard curve [35]. In order to quantify the viral load, DNA samples were evaluated by qPCR using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher, Scientific), according to manufacturer’s instructions to allow detection and quantification of HSV-1 UL39. Cycling conditions were as follows: initial activation 50°C for 2 min, denaturation and Enzyme Activation at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s, hybridization and extension at 60°C for 1 min. The primers for qRT-PCR were the primers for qRT-PCR were Primers (Sense): 5´- CCAACTGCACCATGATCATCGA − 3´, Primers (Anti-sense): 5´- GATGTTTGTCACCGCAACGAA − 3´ and Probe FAM 5’-CCCGCGCACCACGTC-3’ MGB [35,36,37].

Statistical analysis

Information was categorized into distinct categories, referred to as blocks. Block A encompassed general data, Block B covered sociodemographic information, Block C delved into the history of drug and alcohol usage, Block D focused on tuberculosis, Block E examined STI status, and Block F recorded the tests performed. The participants responded to the questionnaires in a “yes” or “no” format. This questionnaire was validated by the Ethics Committee of the UFGD-MS and is included in Supplementary Information. Demographic data, including age, sex, and socioepidemiological information, were analyzed using Pearson’s chi-square test to evaluate their correlation with serological status. Prevalence was established, and the prevalence odds ratio (POR) were estimated to determine the association between sociodemographic variables and HSV-1 positivity among Indigenous people. We assessed the relationship between age, sex, socioeconomic determinants, and HSV-1 infection using Pearson’s chi-square test. Logistic regression was used to estimate the Prevalence odds Ratio (POR). The significance level was set at p < 0.05. Analyses were performed using RStudio software (version 2023.03.0). Maps were constructed using QGIS (version 3.26.1).

Results

In this study, we analyzed 29.1% (1168) of the eligible population over the age of 18 years. Females consisted of a larger proportion of the sample at 80.9% (945/1168), while the males comprised 19.1% (223/1168). The age of the participants ranged from 18 to 103 years for women and 18 to 83 years for men. A high overall prevalence of HSV-1 IgG was observed (97.5%), and in females and males (97.6% and 97.3%, respectively), as shown in Table 1. The distribution of HSV-1 positivity and negativity corresponding to age, where the mean age at infection was 30 years (interquartile range 22.40) years, with the highest HSV-1 prevalence between 29 and 39 years.

Bororó villagers (98.6%, 486/493) had a slightly higher HSV-1 IgG prevalence than Jaguapirú villages (96.7%, 493/653) (POR = 0.42(0.16–0.96; p = 0.07), showing a lower tendency in the Jaguapiru in relation to the Bororo, as shown in Table 1; Fig. 1.

Fig. 1
figure 1

Distribution of anti-HSV-1 IgG positive and negative prevalence in Bororó (right) and Jaguapirú (left) villages (Dourados, Mato Grosso do Sul (MS), Brazil)

Full size image

The prevalence of anti-HSV-1 IgG in Indigenous people who declared to be retired was 97%, lived in another village previously was 97.7%, and had a family allowance of 97.8%, with no statistical significance. The use of cell phones to access health information was reported in 76.5% of participants, in which the HSV-1 IgG prevalence was 97% (POR = 0.23 (0.03–0.79); p < 0.05). Internet use was reported in 21.7% (POR = 0.51 (0.24–1.17) with an HSV-1 IgG prevalence of 96.1%. In participants that did not use cell phones or internet to access health information, the prevalence was 78.3% and 23.5%, respectively. In addition to these technological means, more than half of the participants used television (52%), while 48% did not.

Regarding ethnicities, the anti-HSV-1 IgG prevalence in Guarani-Kaiowá was 98.3% (767/780) (POR = 2.53 (1.2–5.42); p < 0.05); Terena was 96% (214/223) (POR = 0.51 (0.23–1.20), and in other ethnicities (Guarani-Nhandeva, Kadiwéu and Guató) was 82.1%. Guarani-Kaiowá participants were 2.53 more likely to be positive for Anti-HSV-1- IgG than the other ethnicities with statistical significance (Fig. 2).

Fig. 2
figure 2

Positive and negative outcomes for anti-HSV-1 IgG serological tests among Indigenous Guarani-Kaiowá and Terena ethnicities and their geographical locations in Mato Grosso do Sul, Brazil

Full size image

The prevalence of anti-HSV-1 IgG was found to be significantly influenced by Indigenous educational background. The prevalence in illiterate and elementary-educated participants was 98.2% (846/861) (POR = 1.14 (0.17–4.23), high-school-educated was 96.1% (246/256) (POR = 0.49(0.07–1.90) and college-educated was 92.2% (47/51) (POR = 0.23 (0.03–1.24) (Table 1), observing a decrease in the prevalence according to the educational level, and therefore a protective factor with higher educational levels.

Families earning > 5 minimum wages had a 100% prevalence of anti-HSV-1 IgG (10/10) accounting for 0.9% of the total Indigenous population; families earning < 1 minimum wages was 97.4% (666/684%), representing more than half of Indigenous population (58.8%); families earning 1–2 minimum wages was 98.6% (416/422) (POR = 1.87 (0.77–5.2); and families earning > 3 minimum wages was 92% (57/62) (POR = 0.25 (0.09–0.79). The p-value for family income was 0.004, which was statistically significant. Indigenous people who worked formally or informally tested 97.9% for anti-HSV-1 IgG.

Among those who reported condom use behaviors, the prevalence was 14.1% in those who reported ‘always’ used (POR = 0.35 (0.16–0.82), and 85.9% in those who reported ‘sometimes’ or ‘never’ used. In addition, the prevalences in those with risky behaviors and drug and alcohol history are reported in Table 1. The difference in prevalence for former prisoners, alcoholism, illicit drug use, syringe and needle sharers, smoking, and blood transfusions were not statistically significant. Sexual history, sexual activity, sexual intercourse with a partner who was an injection drug user, sexual intercourse with a partner who is an injecting drug user, sex workers, sexual intercourse with HIV carriers, STI history and single sexual partner. However, the prevalence of anti-HSV-1 IgG with respect to sexual preference was 100% in homosexual and 97.6% in heterosexual individuals. Although certain groups may have had higher prevalence rates, these differences were not statistically significant.

Table 1 Comparison of different variables associated with anti-HSV-1 IgG prevalence among Indigenous communities in Bororó and Jaguapirú villages (Dourados, Mato Grosso do Sul (MS), Brazil)
Full size table

In Table 2, the Guarani-Kaiowá ethnic group showed the highest rates of illiteracy (60.8%) and elementary school education (73.3%), which were different from the Guarani-Nhandeva and Terena ethnic groups, where the highest rates of college education were 35.3% and 3.9%, respectively. However, the Guarani-Kaiowá ethnic group had the highest rate with < 1 minimum wage (73.8%), and the Guarani-Nhandeva and Terena ethnic groups had the highest rates of income of 3–4 minimum wages (36.5%) and five minimum wages (10%), respectively. The Guarani-Kaiowá ethnic group had the lowest income and educational level.

Among clinically symptomatic Indigenous individuals, anti-HSV-1 IgM was detected in 17.1%, 21.2%, 12.5%, and 22% of those exhibiting urinary problems, genital wounds, genital warts, and urethral discharge, respectively (Table 3; Fig. 3). Anti-HSV-1 IgM total was 20.8% and found in 21.7% (107/493) and 15.4% (104/675) of Indigenous individuals from Bororó and Jaguapirú villages, respectively. In total, 19.5% (150/767), 18.3% (42/223), and 15% (3/20) of individuals from Guarani-Kaiowá, Terena, and Guarani-Nhandeva, respectively, presented clinical symptoms and reacted to anti-HSV-1 IgM, which resulted in a very high prevalence of HSV-1 in these indigenous villages (Fig. 3). Positive serum anti-HSV-1 IgM tests underwent viral load detection and quantification for qPCR UL39. HSV-1 DNA with high viral loads were detected in participants aged 18–77, with an average of 31.3 (Table 4).

Fig. 3
figure 3

HSV-1 among residents of the Bororó and Jaguapirú villages in Dourados, Mato Grosso do Sul, Brazil

Full size image
Table 2 Relationship between socioeconomic and education variables by ethnic group in Indigenous populations of Dourados, Mato Grosso do Sul (MS), Brazil
Full size table
Table 3 Seroprevalences of anti-HSV-1 IgM in individuals exhibiting clinical symptoms in the Indigenous community, Mato Grosso do Sul, Brazil
Full size table
Table 4 Viral load in participants from Indigenous populations with positive anti-HSV-1 IgM.
Full size table

Discussion

The prevalence of HSV-1 among Indigenous populations can be influenced by various factors that increase vulnerability and risk behaviors. These factors include cultural and social practices pertaining to age, sex, income, education, ethnic background, sex history, sexual partners, and condom use. Moreover, the sharing of contaminated sharp objects, alcoholism, and a history of STIs further contribute to the high prevalence of HSV-1 in this population. It has also been noted that the proximity to national and international borders as well as geographical location could increase the vulnerability of Indigenous populations to HSV-1 infections. Thus, it is imperative to consider these factors when developing effective strategies for STI prevention and control in these communities [38].

The overall prevalence of anti-HSV-1 IgM in the study was 20% (211/1053), varying from 21.7% (107/493) in Bororó to 15.4% (104/675) in Jaguapirú villages. The Guarani-Kaiowá, Terena, and Guarani-Nhandeva groups had anti-HSV-1 IgM prevalence of 19.5% (150/767), 18.3% (42/223), and 15% (3/20), respectively. The most frequent signs and symptoms of STIs were genital wounds, urethral discharge (22%), urinary problems (17.1%), genital sores (21.2%), and genital warts (12.5%). Among the 51 individuals with a history of STIs, 43 (84.3%) tested positive for anti-HSV-1. Further analysis revealed that three individuals with a previous clinical history of genital herpes were anti-HSV-1 positive. According to Sukik, in Latin America and the Caribbean, the virus isolation proportion was approximately 0.9% in genital urethral disease, and 10.9% in genital herpes [33]. These findings provide valuable insights into the prevalence of HSVs among individuals with a history of STIs [34].

Circulation of the virus was confirmed by qPCR: HSV-1 DNA was detected in 25.6% (54/211) of anti-HSV1 IgM-positive samples with viral loads ranging from 5,99E + 02 to 3,36E + 13, indicating a possible HSV-1 outbreak in these Indigenous communities. The high prevalence of HSV-1 is associated with morbidity, physical discomfort, cosmetic disfigurement and psychological distress. In 2014, Arizona had herpes outbreaks that led to exclusion from sporting events [39, 40]. Skin infections are a widespread problem among athletes, causing 8.5% of related adverse events. Wrestlers are particularly susceptible because of the constant skin-to-skin contact required during training and competition. A significant proportion (30%) of viral infections transmitted among high-school wrestlers are caused by HSV-1. These statistics highlight the importance of preventive measures to protect athletes’ health during potential outbreaks and high prevalence [41]. According to Amudha et al., nearly 20% of herpes infections manifest as vesicular or ulcerative lesions in the genital area. Moreover, more than 80% of these patients have no visible symptoms, which makes it easier for the virus to spread and infect healthy individuals. It is important to acknowledge and address these issues to ensure the implementation of effective prevention and management strategies [42, 43]. Based on the results that indicate a high prevalence of HSV-1 IgM in this population, it is reasonable to suggest that an outbreak of HSV-1 has occurred. However, there is no previous data on the prevalence of HSV-1 or other STIs in this population, to make a concrete claim of an HSV-1 outbreak. Furthermore, these findings suggest a significant rate of HSV-1 IgM reactivation or primary infection in this region, which may have contributed to the unexpected increase in infection. These results underscore the importance of continued HSV-1 monitoring in Indigenous communities to prevent its continued spread [44]. According to our analysis, a high percentage (97.5%) of Indigenous Brazilian people residing in Dourados, MS, had HSV-1 IgG antibodies. Furthermore, our research indicates that the prevalence of HSV-1 IgG antibodies is notably similar between the sexes, with females displaying a higher percentage (97.6%) compared to males (97.3%). It was observed that the majority of the people included in the study were women 80.9% (945/1168), while men constituted 19.1% (223/1168). This is due to the demographic location of the villages being located in difficult access to work, health care and, together with this, the social phenomenon on the days when the questionnaires were administered and the blood samples were taken, men, who are the financial providers of the household, were out of the villages due to work or hunting.

Our data also revealed that HSV-1 positivity was most common among those aged 22–40 years in the MS region, with an average age of 30 years at infection. According to Clemens, the prevalence rates for anti-HSV-1 were at 70.1% among 1,037 individuals in the general population of Brazil in 2010, with varying HSV-1 prevalence across different geographic regions of the country, with rates of 73.3% in Manaus, 68.6% in Rio de Janeiro, 62.1% in Porto Alegre, and 47.0% in Fortaleza. These findings provide important insights into the distribution of the virus within the population and may have significant implications for future public health interventions and prevention strategies [34].

A global HSV-1 infection prevalence is well-documented [45]. HSV-1 is responsible for a range of morbidities [39, 46], with orolabial herpes lesions as a typical clinical manifestation [39, 47]. Typically, lifelong, mostly asymptomatic infections are acquired during childhood via oral transmission [46]. However, mounting evidence suggests an HSV-1 epidemiological transition in Europe, North America [13, 47,48,49] and Asia [50], with an increasing number of individuals acquiring the infection through oral sex during adulthood [13, 47, 48]. The report demonstrated that HSV-1 infection is prevalent in Latin America and the Caribbean, with a seroprevalence of 67% in the global population [45]. Nearly 60% of children and 90% of adults are infected, with a high seroprevalence of 76.5% in adults [50, 51] and 96.2% in adults in Africa [52]. The seroprevalence of HSV-1 decreased from 62.0% in 1988–1994 to 57.7% in the year 1999–2004 in United States. Upon analyzing individuals infected with HSV-1 but not HSV-2, a higher percentage during 1999–2004, as compared to 1988–1994, were diagnosed with genital herpes (1.8% vs. 0.4%, respectively; P < 0.01), while the overall trend was positive, suggesting that there may be an upward trend in genital herpes among those solely infected with HSV-1 [51].

An epidemiological transition, defined as significant changes in the occurrence of the infection and/or its mode of transmission, has been observed in multiple Western countries, with HSV-1 becoming the primary cause of first-episode genital herpes, surpassing HSV-2 [13, 45, 47, 49, 53,54,55]. These data suggest the need for region-specific prevention and control strategies to effectively prevent the spread of the disease. According to a study on an Indigenous population in Australia, the HSV-1 seroprevalence was significantly higher, reaching 97.8%. Furthermore, all patients aged 16–24 years included in the study had positive antibodies against HSV-1, which is similar to the rates observed in developing countries where early adult infections approach 100% [56, 57]. In contrast, many developed countries, such as Japan [58], England [59], and the USA [60] report overall seroprevalence rates of HSV-1 to be less than 70%. This is supported by cross-sectional seroprevalence studies that indicate a significant decline in HSV-1 infections in developed countries over the past three decades [61]. This decrease is attributed to advancements in socioeconomic conditions, particularly sanitation and overcrowding, which have greatly reduced childhood acquisition of HSV-1. In a study in the Indigenous Australian population, the findings on HSV-1 were significant for public health decision-making for this population [62, 63].

A study conducted in villages in Dourados City (Mato Grosso do Sul) found that Bororó exhibited a moderately higher prevalence of HSV-1 than Jaguapiró at 98.6% and 96.7%, respectively (POR = 0.42 (0.16–0.96)). Bororó and Jaguapiru villages are predominantly inhabited by the Guarani-Kaiowá and Terena ethnicities [3, 4]. The Bororó population demonstrated higher adherence to the study, perhaps owing to limited access to healthcare services. Bororó village is geographically large, extending from Bolivia to the Miranda River in Brazil [64]. The population’s morbidity rate highlights precarious living conditions and largely comprises infections associated with basic sanitation, hygiene, and alcoholism. Both the Jaguapiró and Bororó Indigenous populations are a mix of families from multiple Indigenous communities, including Guanari-Kaiowá, Guarani-Nhandeva, Terena, Guató, Kadiwéu, Paraguayans, and regional Brazilians assimilated through interethnic marriages [64]. The results of our study indicated that the prevalence of anti-HSV-1 IgG among the Guarani-Kaiowá and Terena ethnicities was 98.3% and 96%, respectively, both of which were significant, with odds ratios of 2.53 (1.2–5.42) and 0.51 (0.23–1.2), respectively. In contrast, the prevalence of anti-HSV-1 IgG in other ethnicities was 82.1%, which was not statistically significant. Our findings suggest that individuals belonging to the Guarani-Kaiowá ethnicity are at a significantly higher risk of acquiring HSV-1 than those belonging to other ethnic groups in the study and are more numerous in the Bororo (72.3%; 405/560) village than in the Jaguapirú village (27.6%; 155/560). However, HSV-1 was more prevalent in the Terena ethnic group in the Jaguapirú village (88%; 92/104) than in the Bororo village (13%; 12/104).

Our study sheds light on the prevalence of HSV-1 among various ethnic groups in Brazil, providing valuable insights into the regional distribution of the disease. As shown in Table 1,the Guarani-Kaiowá ethnic group had the highest percentage of individuals with primary education (73.3%) and higher education (47.1%). However, the Terena and Guarani-Nhandeva ethnic groups had the highest rates of indigenous people with higher education (35.3% and 3.9%, respectively). The Guarani-Kaiowá also had lower income rates than other ethnic groups. This study revealed that the Guarani-Nhandeva and Terena ethnic groups had the highest levels of income and education. In addition, the Terena and Guarani-Kaiowá ethnic groups are located approximately 5 km from the city of Dourados, which allows them access to the urban center and free contact with non-indigenous populations [65]. A negative correlation was observed between the salaries received by indigenous people and the prevalence of HSV-1. When individuals earned between 1 and 2 minimum wages, their chance of acquiring the virus increased, with a prevalence of 98.6% (POR = 1.87 (0.77–5.2). Poverty, low income, limited access to medical care and limited information contribute to the high prevalence of HSV-1 in vulnerable populations, such as indigenous communities [64, 65].

The Indigenous population of Cape York in Australia is confronting unwarranted economic and social discrepancies such as elevated unemployment rates and cramped living conditions, along with lower household incomes and a reduced likelihood of completing secondary education [66]. These variables may be linked to the increased prevalence of HSV-1 infection in this vulnerable community. Further research is necessary to unravel the specific underlying factors that contribute to HSV-1 acquisition in this group.

With the advent of technology, electronic devices act as gateways for information and healthcare services, and access to these media has been linked to a higher HSV-1 prevalence [67,68,69,70]. In individuals who used cell phones, television, and the internet within Indigenous villages, the prevalence was 97% (POR = 0.23 (0.03–0.79)), 97%, and 96.1% (POR = 0.51 (0.24–1.17)), respectively, compared to non-device-users. Among these villages, 76.5% (867/894), 52% (589/607), and 21.7% (244/254) reported using cell phones, television, and the internet, respectively. However, those without access to communication media had a higher chance of acquiring HSV-1. Therefore, limited access to information and diagnostic services may be responsible for the higher prevalence of HSV-1 infection in Indigenous communities. Notably, the remoteness of some villages can affect media access, underscoring the importance of promoting disease prevention and awareness through improved communication channels [70]. The prevalence of anti-HSV-1 IgG among Indigenous individuals moving between villages was 97%. However, compared to those who refrained from travelling and migration, the risk of having anti-HSV-1 IgG was only 3% greater which was not statistically significant.

The results indicated a significantly higher prevalence of HSV-1 among smokers (97.9%) than among nonsmokers (2.1%), providing evidence that smoking is a risk factor for contracting HIV, STIs, and Tuberculosis, separate from poverty and alcohol use [71,72,73]. Further analysis revealed that Indigenous individuals who reported always using condoms (94.5%) were less affected than those who did not use or used sometimes (98%). Additionally, condom use was protective in nature in preventing STI transmission and statistically associated. The low condom use reported among the population may contribute to increased STI rates; even though saliva is the primary means of HSV-1 transmission, sexual transmission can also occur [74,75,76,77,78,79]. This study also found an association between a low frequency of consistent condom use and vulnerability to the transmission of other STIs among Indigenous migrant agricultural workers with same-ethnic partners, concurrent partners, and partners using illegal drugs. These findings emphasize the importance of promoting condom use as an effective measure for preventing STIs, especially in vulnerable populations [78,79,80].

According to our study, the presence of risky behaviors and a history of drug and alcohol use were linked to the acquisition of HSV-1. Our study showed a high prevalence of HSV-1 among individuals who had a former inmate status, received blood transfusions, engaged in illicit drug use, were alcoholics, and shared syringes and needle objects. We observed prevalence rates of 96%, 97.9%, 97.7%, 97.8%, and 94.7%, respectively; however, there were no statistically significant differences between them. HSV-1 infections associated with blood transfusions are more likely to occur in severely immunocompromised individuals, often leading to fatal generalized infections [27].

In this study, we identified additional risk factors contributing to a higher likelihood of acquiring and transmitting HSV-1. Sexual behaviors such as sex work, engaging in sexual encounters outside one’s cultural context, and experiencing repeated breakups are among these factors. Based on our analysis of Indigenous cultural behaviors, we found that certain sexual practices place individuals at a particularly high risk of HSV-1 infection [77, 81,82,83], such as sexual intercourse with a partner who is an injecting drug user, sex worker, STI history, HIV carriers and non-injecting illicit drug user, were risk factors for HSV-1 with rates of 96.6%, 90.9%, 94.1%, 100% and 100% of cases, respectively. These findings highlight the importance of targeted interventions and education to reduce the spread of HSV-1 in these populations. In sexual preference studies, a considerable proportion of Indigenous individuals who were identified as sexually active homosexuals exhibited a 100% prevalence rate for hsv-1, compared to their heterosexual counterparts who exhibited a prevalence rate of 97.6%. A study conducted in Melbourne, Australia revealed that 73.3% of HIV-seronegative homosexual males possessed HSV-1 antibodies [84], this study, indicating a significantly high prevalence (100%) for HSV-1 in the indigenous homosexual population in Brazil. These findings contribute to a better understanding of the impact of sexual preference on the prevalence of HSV-1 and can guide and prioritize future preventive measures. Despite our best efforts, the scope of this study was constrained due to the scarcity of blood samples required for molecular and phylogenetic analysis. These precious materials were procured from populations that are notoriously hard to reach, contributing to the challenges faced during our investigation.

Conclusions

The prevalence of HSV-1 in Indigenous populations was significantly higher (97.5%) than in the general adult Brazilian population (70.1%). Our study also identified a high incidence of HSV-1 infection and circulation of HSV-1 DNA within the Jaguapirú and Bororú village populations. The discovery of HSV-1 IgM suggests that reactivation or primary infection of HSV-1 may have caused an outbreak of the virus in the Bororó and Jaguapirú Indigenous populations, due to its high HSV1 prevalence. It is important to emphasize that HSV-1 is a latent virus that can be reactivated and, in some cases, cause serious neurological complications. If left untreated, widespread and disseminated HSV-1 infections can lead to high mortality rates in high-risk groups. Several socioeconomic factors, sexual behaviors, and drug use contribute to the increased HSV-1 prevalence in Indigenous population in Brazil. As such, culturally appropriate intervention programs are necessary to eliminate health access barriers faced by Indigenous populations. Additionally, public health policies should aim to promote information on HSV-1 infections and therapeutically prevent and control the virus among the Indigenous populations in Brazil. Achieving these goals requires a respectful and culturally sensitive approach, including the promotion of appropriate condom usage. Our research offers valuable insights into the prevalence and potential risk factors contributing to HSV-1 infections among Indigenous populations in Brazil, providing more effective public health policies and interventions. In conclusion, it is important to acknowledge that the health requirements and susceptibilities of Indigenous Peoples are diverse, given that they represent unique and multifaceted communities inhabiting varying environmental and social conditions.

Data availability

The data presented in this study are available upon request from the corresponding author. All the data from this study are reported in this article.

Abbreviations

HSV-1:

Human Alphaherpesvirus 1

MS:

Mato Grosso do Sul

IBGE:

Geography and Statistics

FUNAI:

National Indian Foundation

STIs:

Sexually transmitted infections

HIV-1:

Human immunodeficiency virus-1

UFGD-MS:

University of Grande Dourados

SIHD/MS:

Special Indigenous Health District of Mato Grosso do Sul

IgM:

Anti-HSV-1 (gG1) type M

IgG:

Anti-HSV-1 (gG1) type G

POR:

Prevalence Odds Ratio

References

  1. WHO, AAC, SEVENTY-SIXTH WORLD, HEALTH ASSEMBLY World Health Organization (WHO). The health of Indigenous PeoplesDraft resolution proposed by Australia, Bolivia (Plurinational State of), Brazil, Canada, Colombia, Cuba, Ecuador, European Union and its Member States, Guatemala, Mexico, New Zealand, Panama, Paraguay, Peru, United States of America and Vanuatu. Agenda item 16.3: A/RES/61/295; 23 May 2023.

  2. Brazilian Geographic and Statistics Institute. IBGE. Indigenous in the 2010population census. https://indigenas.ibge.gov.br/images/indigenas/estudos/indig ena_censo 2010.pdf.2010.

  3. Cidadania. SMSEd. Governo do Estado de Mato Grosso do Sul.: https://www.secid.ms.gov.br/ comunidades- indig enas-2/.

  4. de Alcantara MLB, Moure W, Trajber Z, Machado IR. Equipe De Jovens Da Ação dos Jovens Indígenas De Dourados-MS. a percepção do suicídio como inseparável das outras formas de violência segundo os/as jovens indígenas: um estudo de caso da Reserva Indígena De Dourados. Rev Med. 2020;99(3):305–18.

    Google Scholar 

  5. Mota JGB, Cavalcante TLV. Reserva Indígena de Dourados: Histórias e Desafios Contemporâneos, 285 (Karywa, 2019). ISBN: 978-85-68730–38–6. 2019.

  6. Kotronias D, Kapranos N. Detection of herpes simplex virus DNA in maternal breast milk by in situ hybridization with tyramide signal amplification. Vivo. 1999;13(6):463–6.

    CAS  Google Scholar 

  7. Braço ILJ, de Sá KSG, Waqasi M, Queiroz MAF, da Silva ANR, Cayres-Vallinoto IMV, et al. High prevalence of human T-lymphotropic virus 2 (HTLV-2) infection in villages of the Xikrin tribe (Kayapo), Brazilian Amazon region. BMC Infect Dis. 2019;19(1):459.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Graeff SV, Pícolli RP, Arantes R, Castro VOL, Cunha RVD. Epidemiological aspects of HIV infection and AIDS among indigenous populations. Rev Saude Publica. 2019;53:71.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Vallinoto AC, Ishak MO, Azevedo VN, Vicente AC, Otsuki K, Hall WW, et al. Molecular epidemiology of human T-lymphotropic virus type II infection in Amerindian and urban populations of the Amazon region of Brazil. Hum Biol. 2002;74(5):633–44.

    Article  PubMed  Google Scholar 

  10. SANTOS VL. Analyzing STD and aids control policies among Brazilian indigenous population. Tempus Actas de Saúde Coletiva; 2010.

  11. Romero-Sandoval N, Cifuentes L, León G, Lecaro P, Ortiz-Rico C, Cooper P, et al. High rates of exposures to Waterborne pathogens in Indigenous communities in the Amazon Region of Ecuador. Am J Trop Med Hyg. 2019;101(1):45–50.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Gabster A, Pascale JM, Cislaghi B, Francis SC, Weiss HA, Martinez A, et al. High prevalence of sexually transmitted infections, and high-risk sexual behaviors among indigenous adolescents of the Comarca Ngäbe-Buglé, Panama. Sex Transm Dis. 2019;46(12):780–7.

    Article  PubMed  Google Scholar 

  13. Roberts CM, Pfister JR, Spear SJ. Increasing proportion of herpes simplex virus type 1 as a cause of genital herpes infection in college students. Sex Transm Dis. 2003;30(10):797–800.

    Article  PubMed  Google Scholar 

  14. Placek BJ, Berger SL. Chromatin dynamics during herpes simplex virus-1 lytic infection. Biochim Biophys Acta. 2010;1799(3–4):223–7.

    Article  CAS  PubMed  Google Scholar 

  15. j CA. D I. Management of herpes simplex virus infections. Paediatr Child H2004.

  16. Tsatsos M, MacGregor C, Athanasiadis I, Moschos MM, Jameel S, Hossain P, et al. Herpes simplex virus keratitis: an update of the pathogenesis and current treatment with oral and topical antiviral agents - comment. Clin Exp Ophthalmol. 2017;45(9):932.

    Article  PubMed  Google Scholar 

  17. Steiner I, Benninger F. Update on herpes virus infections of the nervous system. Curr Neurol Neurosci Rep. 2013;13(12):414.

    Article  PubMed  Google Scholar 

  18. Marcocci ME, Napoletani G, Protto V, Kolesova O, Piacentini R, Li Puma DD, et al. Herpes simplex Virus-1 in the brain: the Dark side of a sneaky infection. Trends Microbiol. 2020;28(10):808–20.

    Article  CAS  PubMed  Google Scholar 

  19. Pinninti SG, Kimberlin DW. Neonatal herpes simplex virus infections. Semin Perinatol. 2018;42(3):168–75.

    Article  PubMed  Google Scholar 

  20. Laval K, Enquist LW. The potential role of Herpes Simplex Virus Type 1 and Neuroinflammation in the pathogenesis of Alzheimer’s Disease. Front Neurol. 2021;12:658695.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Desai DV, Kulkarni SS. Herpes Simplex Virus: the interplay between HSV, Host, and HIV-1. Viral Immunol. 2015;28(10):546–55.

    Article  CAS  PubMed  Google Scholar 

  22. Johnston C, Corey L. Current concepts for genital herpes simplex virus infection: Diagnostics and Pathogenesis of Genital Tract Shedding. Clin Microbiol Rev. 2016;29(1):149–61.

    Article  CAS  PubMed  Google Scholar 

  23. Davenport DS, Johnson DR, Holmes GP, Jewett DA, Ross SC, Hilliard JK. Diagnosis and management of human B virus (Herpesvirus Simiae) infections in Michigan. Clin Infect Dis. 1994;19(1):33–41.

    Article  CAS  PubMed  Google Scholar 

  24. Fierer J, Bazely P, Braude AI. Herpes B virus encephalomyelitis presenting as ophthalmic zoster. A possible latent infection reactivated. Ann Intern Med. 1973;79(2):225–8.

    Article  CAS  PubMed  Google Scholar 

  25. HOLMES AW, CALDWELL RG, DEDMON RE DEINHARDTF. ISOLATION AND CHARACTERIZATION OF A NEW HERPES VIRUS. J Immunol. 1964;92:602–10.

    Article  CAS  PubMed  Google Scholar 

  26. Roizman B, Knipe D, Whitley R. Herpes simplex viruses. In: Knipe DM, Howley PM, editors. Fields Virology. 5th ed. Philadelphia: LippincottWilliams & Wilkins; 2007. pp. 2502–601.

    Google Scholar 

  27. Whitley R. Herpes simplex virus. In: Scheld WM, Whitley RJ, Marra CM, editors. Infections of the central nervous system. 4th ed. Philadelfia,: Lippincott Williams & Wilkins; 2014. pp. 137–56.

    Google Scholar 

  28. Freeman EE, Weiss HA, Glynn JR, Cross PL, Whitworth JA, Hayes RJ. Herpes simplex virus 2 infection increases HIV acquisition in men and women: systematic review and meta-analysis of longitudinal studies. AIDS. 2006;20(1):73–83.

    Article  PubMed  Google Scholar 

  29. Corey L, Wald A, Celum CL, Quinn TC. The effects of herpes simplex virus-2 on HIV-1 acquisition and transmission: a review of two overlapping epidemics. J Acquir Immune Defic Syndr. 2004;35(5):435–45.

    Article  PubMed  Google Scholar 

  30. Cohen EM, Avital N, Shamay M, Kobiler O. Abortive herpes simplex virus infection of nonneuronal cells results in quiescent viral genomes that can reactivate. Proc Natl Acad Sci U S A. 2020;117(1):635–40.

    Article  CAS  PubMed  Google Scholar 

  31. James C, Harfouche M, elton NJ, Turner KME, Abu-Raddad LJ, SL G et al. Supplementary webappendix: data from 2016 HSV infection estimates (data repository). Bristol: University of Bristol; 2019. Bristol: University of Bristol; 2019.2019.

  32. James C, Harfouche M, Welton NJ, Turner KM, Abu-Raddad LJ, Gottlieb SL, et al. Herpes simplex virus: global infection prevalence and incidence estimates, 2016. Bull World Health Organ. 2020;98(5):315–29.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Sukik L, Alyafei M, Harfouche M, Abu-Raddad LJ. Herpes simplex virus type 1 epidemiology in Latin America and the Caribbean: systematic review and meta-analytics. PLoS ONE. 2019;14(4):e0215487.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Clemens SA, Farhat CK. Seroprevalence of herpes simplex 1–2 antibodies in Brazil. Rev Saude Publica. 2010;44(4):726–34.

    Article  PubMed  Google Scholar 

  35. da Silva AS, Raposo JV, Pereira TC, Pinto MA, de Paula VS. Effects of RNA interference therapy against herpes simplex virus type 1 encephalitis. Antivir Ther. 2016;21(3):225–35.

    Article  PubMed  Google Scholar 

  36. Zhe R, Mei-Ying Z, Kitazato K, Kobayash N, Qin-Chang Z, Pei-Zhuo Z, et al. Effect of siRNA on HSV-1 plaque formation and relative expression levels of UL39 mRNA. Arch Virol. 2008;153(7):1401–6.

    Article  PubMed  Google Scholar 

  37. Lima LR, Silva AP, Schmidt-Chanasit J, Paula VS. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction. Mem Inst Oswaldo Cruz. 2017;112(3):220–3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Ministério de Saúde. Povos indígenas e a prevenção as DST, HIV e aids: Manual de diretrizes técnicas. ISBN 85-334-0232-5; 2000.

  39. C BR, I BD. Treatment of herpes simplex virus infections. Antiviral Res2004.

  40. Anderson BJ. The effectiveness of valacyclovir in preventing reactivation of herpes gladiatorum in wrestlers. Clin J Sport Med. 1999;9(2):86–90.

    Article  CAS  PubMed  Google Scholar 

  41. Yard EE, Collins CL, Dick RW, Comstock RD. An epidemiologic comparison of high school and college wrestling injuries. Am J Sports Med. 2008;36(1):57–64.

    Article  PubMed  Google Scholar 

  42. Amudha VP, Rashetha, Sucilathangam G, Cinthujah B, Revathy C. Serological Profile of HSV-2 in STD patients: evaluation of Diagnostic Utility of HSV-2 IgM and IgG detection. J Clin Diagn Res. 2014;8(12):DC16–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Carvalho M, de Carvalho S, Pannuti CS, Sumita LM, de Souza VA. Prevalence of herpes simplex type 2 antibodies and a clinical history of herpes in three different populations in Campinas City, Brazil. Int J Infect Dis. 1998;3(2):94–8.

    Article  PubMed  Google Scholar 

  44. Agabi YA, Banwat EB, Mawak JD, Lar PM, Dashe N, Dashen MM, et al. Seroprevalence of herpes simplex virus type-2 among patients attending the Sexually Transmitted Infections Clinic in Jos, Nigeria. J Infect Dev Ctries. 2010;4(9):572–5.

    Article  PubMed  Google Scholar 

  45. Looker KJ, Magaret AS, May MT, Turner KM, Vickerman P, Gottlieb SL, et al. Global and Regional estimates of prevalent and incident herpes simplex virus type 1 infections in 2012. PLoS ONE. 2015;10(10):e0140765.

    Article  PubMed  PubMed Central  Google Scholar 

  46. Fatahzadeh M, Schwartz RA. Human herpes simplex virus infections: epidemiology, pathogenesis, symptomatology, diagnosis, and management. J Am Acad Dermatol. 2007;57(5):737–63. quiz 64 – 6.

    Article  PubMed  Google Scholar 

  47. Bernstein DI, Bellamy AR, Hook EW, Levin MJ, Wald A, Ewell MG, et al. Epidemiology, clinical presentation, and antibody response to primary infection with herpes simplex virus type 1 and type 2 in young women. Clin Infect Dis. 2013;56(3):344–51.

    Article  CAS  PubMed  Google Scholar 

  48. Ayoub HH, Chemaitelly H, Abu-Raddad LJ. Characterizing the transitioning epidemiology of herpes simplex virus type 1 in the USA: model-based predictions. BMC Med. 2019;17(1):57.

    Article  PubMed  PubMed Central  Google Scholar 

  49. Löwhagen GB, Tunbäck P, Andersson K, Bergström T, Johannisson G. First episodes of genital herpes in a Swedish STD population: a study of epidemiology and transmission by the use of herpes simplex virus (HSV) typing and specific serology. Sex Transm Infect. 2000;76(3):179–82.

    Article  PubMed  PubMed Central  Google Scholar 

  50. Khadr L, Harfouche M, Omori R, Schwarzer G, Chemaitelly H, Abu-Raddad LJ. The epidemiology of herpes simplex virus type 1 in Asia: systematic review, Meta-analyses, and Meta-regressions. Clin Infect Dis. 2019;68(5):757–72.

    Article  PubMed  Google Scholar 

  51. Xu F, Sternberg MR, Kottiri BJ, McQuillan GM, Lee FK, Nahmias AJ, et al. Trends in herpes simplex virus type 1 and type 2 seroprevalence in the United States. JAMA. 2006;296(8):964–73.

    Article  CAS  PubMed  Google Scholar 

  52. Harfouche M, Chemaitelly H, Abu-Raddad LJ. Herpes simplex virus type 1 epidemiology in Africa: systematic review, meta-analyses, and meta-regressions. J Infect. 2019;79(4):289–99.

    Article  PubMed  Google Scholar 

  53. Nilsen A, Myrmel H. Changing trends in genital herpes simplex virus infection in Bergen, Norway. Acta Obstet Gynecol Scand. 2000;79(8):693–6.

    Article  CAS  PubMed  Google Scholar 

  54. Samra Z, Scherf E, Dan M. Herpes simplex virus type 1 is the prevailing cause of genital herpes in the Tel Aviv area, Israel. Sex Transm Dis. 2003;30(10):794–6.

    Article  PubMed  Google Scholar 

  55. Gilbert C, Bestman-Smith J, Boivin G. Resistance of herpesviruses to antiviral drugs: clinical impacts and molecular mechanisms. Drug Resist Updat. 2002;5(2):88–114.

    Article  CAS  PubMed  Google Scholar 

  56. Haddow LJ, Sullivan EA, Taylor J, Abel M, Cunningham AL, Tabrizi S, et al. Herpes simplex virus type 2 (HSV-2) infection in women attending an antenatal clinic in the South Pacific island nation of Vanuatu. Sex Transm Dis. 2007;34(5):258–61.

    Article  PubMed  Google Scholar 

  57. Smith JS, Robinson NJ. Age-specific prevalence of infection with herpes simplex virus types 2 and 1: a global review. J Infect Dis. 2002;186(Suppl 1):S3–28.

    Article  PubMed  Google Scholar 

  58. Hashido M, Lee FK, Nahmias AJ, Kawana T. Prevalence of herpes simplex virus type 1- and 2- specific antibodies among the acute, recurrent, and provoked types of female genital herpes. Microbiol Immunol. 1997;41(10):823–7.

    Article  CAS  PubMed  Google Scholar 

  59. Vyse AJ, Gay NJ, Slomka MJ, Gopal R, Gibbs T, Morgan-Capner P, et al. The burden of infection with HSV-1 and HSV-2 in England and Wales: implications for the changing epidemiology of genital herpes. Sex Transm Infect. 2000;76(3):183–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Fleming DT, McQuillan GM, Johnson RE, Nahmias AJ, Aral SO, Lee FK, et al. Herpes simplex virus type 2 in the United States, 1976 to 1994. N Engl J Med. 1997;337(16):1105–11.

    Article  CAS  PubMed  Google Scholar 

  61. Tunbäck P, Bergström T, Andersson AS, Nordin P, Krantz I, Löwhagen GB. Prevalence of herpes simplex virus antibodies in childhood and adolescence: a cross-sectional study. Scand J Infect Dis. 2003;35(8):498–502.

    Article  PubMed  Google Scholar 

  62. Ades AE, Peckham CS, Dale GE, Best JM, Jeansson S. Prevalence of antibodies to herpes simplex virus types 1 and 2 in pregnant women, and estimated rates of infection. J Epidemiol Community Health. 1989;43(1):53–60.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  63. Nahmias AJ, Lee FK, Beckman-Nahmias S. Sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. Scand J Infect Dis Suppl. 1990;69:19–36.

    CAS  PubMed  Google Scholar 

  64. BRASIL. Instituto Brasileiro de Geografia e Estatística (Cidades e Estados Dourados, 2022). https://www.ibge.gov.br/cidades-e-estados/ms/dourados.html.2022.

  65. Coelho RA. Composição da População Segundo a Cor no Brasil e Nas Diversas Regiões Fisiográficas e Unidades da Federação.In ONTRIBUIÇÕES Para o Estudo da Demografia do Brasil. (Estudos de Estatística Teórica e Aplicada). 1970.

  66. Weiss H. Epidemiology of herpes simplex virus type 2 infection in the developing world. Herpes. 2004;11(Suppl 1):A24–35.

    Google Scholar 

  67. Obasi A, Mosha F, Quigley M, Sekirassa Z, Gibbs T, Munguti K, et al. Antibody to herpes simplex virus type 2 as a marker of sexual risk behavior in rural Tanzania. J Infect Dis. 1999;179(1):16–24.

    Article  CAS  PubMed  Google Scholar 

  68. Miskulin M, Miskulin I, Milas J, Antolović-Pozgain A, Rudan S, Vuksić M. Prevalence and risk factors for herpes simplex virus type 2 infections in East Croatia. Coll Antropol. 2011;35(1):9–14.

    PubMed  Google Scholar 

  69. Stanberry LR, Rosenthal SL, Mills L, Succop PA, Biro FM, Morrow RA, et al. Longitudinal risk of herpes simplex virus (HSV) type 1, HSV type 2, and cytomegalovirus infections among young adolescent girls. Clin Infect Dis. 2004;39(10):1433–8.

    Article  PubMed  Google Scholar 

  70. Engels D, Zhou XN. Neglected tropical diseases: an effective global response to local poverty-related disease priorities. Infect Dis Poverty. 2020;9(1):10.

    Article  PubMed  PubMed Central  Google Scholar 

  71. van Zyl Smit RN, Pai M, Yew WW, Leung CC, Zumla A, Bateman ED, et al. Global lung health: the colliding epidemics of tuberculosis, tobacco smoking, HIV and COPD. Eur Respir J. 2010;35(1):27–33.

    Article  PubMed  PubMed Central  Google Scholar 

  72. Halsey NA, Coberly JS, Holt E, Coreil J, Kissinger P, Moulton LH, et al. Sexual behavior, smoking, and HIV-1 infection in Haitian Women. JAMA. 1992;267(15):2062–6.

    Article  CAS  PubMed  Google Scholar 

  73. Penkower L, Dew MA, Kingsley L, Becker JT, Satz P, Schaerf FW, et al. Behavioral, health and psychosocial factors and risk for HIV infection among sexually active homosexual men: the Multicenter AIDS Cohort Study. Am J Public Health. 1991;81(2):194–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. Gupta R, Warren T, Wald A. Genital herpes. Lancet. 2007. https://doi.org/10.1016/S0140-6736(07)61908-4.

    Article  PubMed  Google Scholar 

  75. Garland SM, Steben M. Genital herpes. Best Pract Res Clin Obstet Gynaecol. 2014;28(7):1098–110.

    Article  PubMed  Google Scholar 

  76. Hollier LM. H. S. Genital herpes. BMJ Clin Evid2011.

  77. Shangase N, Kharsany ABM, Ntombela NP, Pettifor A, McKinnon LR. A systematic review of Randomized controlled trials of School based interventions on sexual risk behaviors and sexually transmitted infections among Young adolescents in Sub-saharan Africa. AIDS Behav. 2021;25(11):3669–86.

    Article  PubMed  PubMed Central  Google Scholar 

  78. Magaret AS, Mujugira A, Hughes JP, Lingappa J, Bukusi EA, DeBruyn G, et al. Effect of Condom Use on per-act HSV-2 transmission risk in HIV-1, HSV-2-discordant couples. Clin Infect Dis. 2016;62(4):456–61.

    PubMed  Google Scholar 

  79. Sarmati L, Babudieri S, Longo B, Starnini G, Carbonara S, Monarca R, et al. Human herpesvirus 8 and human herpesvirus 2 infections in prison population. J Med Virol. 2007;79(2):167–73.

    Article  CAS  PubMed  Google Scholar 

  80. Caballero-Hoyos JR, Monárrez-Espino J. Concurrence and selection of sexual partners as predictors of condom use among Mexican indigenous migrant workers. Rev Salud Publica (Bogota). 2018;20(3):293–300.

    Article  PubMed  Google Scholar 

  81. Monsell E, McLuskey J. Factors influencing STI transmission in middle-aged heterosexual individuals. Br J Nurs. 2016;25(12):676–80.

    Article  PubMed  Google Scholar 

  82. Carey C, O’Donnell K, Davoren M, Quinlan M, Igoe D, Barrett P. Factors associated with lower knowledge of HIV and STI transmission, testing and treatment among MSM in Ireland: findings from the MSM Internet Survey Ireland (MISI) 2015. Sex Transm Infect. 2021;97(5):351–6.

    Article  PubMed  Google Scholar 

  83. Bozicević I, Stulhofer A, Ajduković D, Kufrin K. Patterns of sexual behaviour and reported symptoms of STI/RTIs among young people in Croatia–implications for interventions’ planning. Coll Antropol. 2006;30(Suppl 2):63–70.

    PubMed  Google Scholar 

  84. Brazzale AG, Russell DB, Cunningham AL, Taylor J, McBride WJ. Seroprevalence of herpes simplex virus type 1 and type 2 among the indigenous population of Cape York, Far North Queensland, Australia. Sex Health. 2010;7(4):453–9.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank all participants for their contributions to our study.

Funding

This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Oswaldo Cruz Institute and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and partially funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Grants 440245/2018-4), Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT 041/2017), and Secretaria do Estado de Saúde of Mato Grosso do Sul.

Author information

Authors and Affiliations

  1. Molecular Virology and Parasitology Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, 21040360, Brazil

    Flávia Freitas de Oliveira Bonfim, Jéssica Gonçalves Pereira, Ana Carolina Silva Guimarães & Vanessa Salete de Paula

  2. Viral Hepatitis Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, 21040360, Brazil

    Livia Melo Villar

  3. Department of Epidemiology of Microbial Diseases, Universidade Federal de Mato Grosso do Sul-UFMS, Campo Grande, Mato Grosso do Sul, Brazil

    Julio Croda

  4. Fiocruz Mato Grosso do Sul, Fundação Oswaldo Cruz, Campo Grande, Mato Grosso do Sul, Brazil

    Julio Croda

  5. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA

    Crhistinne Cavalheiro Maymone Gonçalves

  6. Binacional Campus of Oiapoque, Federal University of Amapá, Amapá, 68903419, Brazil

    Solange Rodrigues da Silva

  7. Center for Biological and Health Sciences, Blood Center Sector, Federal University of Mato Grosso do Sul, Campo Grande, Grosso do Sul, 79070900, Brazil

    Grazielli Rocha de Rezende Romeira, Gabriela Alves Cesar, Sabrina Weis-Torres & Ana Rita Coimbra Motta-Castro

  8. State Department of Health of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79031350, Brazil

    Crhistinne Cavalheiro Maymone Gonçalves & Ana Rita Coimbra Motta-Castro

  9. Laboratory of Tryposomatid Biology, Oswaldo Cruz Foundation, Rio de Janeiro, 21040360, Brazil

    Lucas Fernando Tinoco Leonardo

  10. Oswaldo Cruz Foundation, Biosafety Level 3 Facility (BSL-3), Rio de Janeiro, 21040360, Brazil

    Marco Aurélio Horta

  11. Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados, Mato Grosso do Sul, Brazil

    Simone Simionatto

  12. Environmental Analysis Laboratory, Southern Cross University, Military Road East, Lismore, NSW, 2480, Australia

    Vivianne de Oliveira Landgraf de Castro

Authors
  1. Flávia Freitas de Oliveira Bonfim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Livia Melo Villar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Julio Croda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jéssica Gonçalves Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ana Carolina Silva Guimarães
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Solange Rodrigues da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Crhistinne Cavalheiro Maymone Gonçalves
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lucas Fernando Tinoco Leonardo
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Grazielli Rocha de Rezende Romeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Gabriela Alves Cesar
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Sabrina Weis-Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Vivianne de Oliveira Landgraf de Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Marco Aurélio Horta
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Simone Simionatto
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Ana Rita Coimbra Motta-Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Vanessa Salete de Paula
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization, F.F.d.O.B., L.M.V., S.S., J.C., A.R.C.M.-C. and V.S.d.P.; methodology, F.F.d.O.B., M.A.H., S.R.d.S. and V.S.d.P.; Maps, S.R.d.S.; L.F.T.L and F.F.d.O.B.; investigation, F.F.d.O.B., V.S.d.P., S.S., A.R.C.M.-C.; A.C.S.G; J.G.P. and L.M.V.; resources, F.F.d.O.B., J.C., C.C.M.G., V.d.O.L.d.C., G.R.d.R.R., G.A.C., S.M.d.S.W.-T., S.S. and A.R.C.M.-C. and V.S.d.P.; data curation, A.R.C.M.-C., S.S., M.A.H. and V.S.d.P.; writing?original draft preparation, F.F.d.O.B.; writing review and editing; supervision, V.S.d.P., project administration, F.F.d.O.B., S.S., J.C., A.R.C.M.-C. and V.S.d.P.; funding acquisition, V.S.d.P., S.S., J.C., A.R.C.M.-C. and L.M.V. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Vanessa Salete de Paula.

Ethics declarations

Ethics approval and consent to participate

Approval was received from the Ethics Committee of the University of Grande Dourados (UFGD-MS) in March 2017 (CA AE:62012616.3.0000.5160 (number 2.000.496)) .

Consent for publication

Not applicable.

Informed consent Statement

Informed consent was obtained from all participants involved in the study.

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

de Oliveira Bonfim, F.F., Villar, L.M., Croda, J. et al. High silent prevalence of human herpesvirus 1 (HSV-1) infection affecting the indigenous reservation of the municipality of Dourados, Central-West Brazil. BMC Infect Dis 24, 700 (2024). https://doi.org/10.1186/s12879-024-09497-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12879-024-09497-5

Keywords

BMC Infectious Diseases

ISSN: 1471-2334

Contact us