Table 4 Consistency of the pentaplex qRT-PCR and singleplex qPCR for detecting blood samples
| Â | No. (%) positive numbers in blood donors | No. (%) positive numbers in clinical samples | ||||||
|---|---|---|---|---|---|---|---|---|
Subject | HBV | HCV | HEV | T. pallidum | HBV | HCV | HEV | T. pallidum |
Pentaplex qRT-PCR | 13(1.08) | 3(0.25) | 1(0.08) | 0(0) | 56(4.67) | 9(0.75) | 0(0) | 0(0) |
Singleplex qPCR | 13(1.08) | 3(0.25) | 1(0.08) | 0(0) | 56(4.67) | 9(0.75) | 0(0) | 0(0) |
Sensitivity (% ) | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
Specificity (% ) | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
Agreement (% ) | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |