Table 2 The limit of detection of the pentaplex qRT-PCR
Pathogen | Concentration (copies/µL) | Reactions | Positive | Hit rate(%) | 95%LOD (copies/µL) |
|---|---|---|---|---|---|
HBV | 100 | 20 | 20 | 100 | 7.11 |
50 | 20 | 20 | 100 | ||
10 | 20 | 20 | 100 | ||
5 | 20 | 17 | 85 | ||
1 | 20 | 14 | 70 | ||
HCV | 100 | 20 | 20 | 100 | 7.65 |
50 | 20 | 20 | 100 | ||
10 | 20 | 20 | 100 | ||
5 | 20 | 16 | 80 | ||
1 | 20 | 11 | 55 | ||
HEV | 100 | 20 | 20 | 100 | 8.45 |
50 | 20 | 20 | 100 | ||
10 | 20 | 20 | 100 | ||
5 | 20 | 14 | 70 | ||
1 | 20 | 8 | 40 | ||
T. pallidum | 100 | 20 | 20 | 100 | 9.06 |
50 | 20 | 20 | 100 | ||
10 | 20 | 20 | 100 | ||
5 | 20 | 10 | 50 | ||
1 | 20 | 3 | 15 |