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Table 1 Primers and probes for detecting HBV, HCV, HEV, T. pallidum, and RNase P

From: Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene

Species

Gene target

Primer/probe

Primer/probe sequence

Length (bp)

Product size(bp)

Concentration((µM)

HBV

S

Forward

5′-TGTGTCTGCGGCGTTTTATC-3′

20

139

0.2

 

S

Reverse

5′-CATGGTCCCGTGCTGGTAG-3′

19

0.2

 

S

Probe

5′-VIC-ATCCTGCTGCTATGCCTCATCTTCTTG-BHQ1-3′

27

0.2

HCV

5’ UTR

Forward

5′-TAGCCGAGTAGTGTTGGGTC-3′

20

95

0.1

 

5’ UTR

Reverse

5′-TCATGGTGCACGGTCTACG-3′

19

0.1

 

5’ UTR

Probe

5′-CY5-TACTGCCTGATAGGGTGCTTGCG-BHQ3-3′

23

0.1

HEV

ORF2

Forward

5′-ATCTTGCTGACACGCTTCTCG-3′

21

158

0.4

 

ORF2

Reverse

5′-AGCTATACCCTTATCCTGCTGA-3′

22

0.4

 

ORF2

Probe

5′-TAMRA-GTCTCAGCCAATGGCGAGCCGAC-BHQ2-3′

23

0.4

T.pallidum

TPP47

Forward

5′-AGGTAAGCAGCATGGAGAGC-3′

20

117

0.1

 

TPP47

Reverse

5′-AGCCATCAGCCCTTTTCAGC-3′

20

0.1

 

TPP47

Probe

5′-FAM-CCGCACGACCTTGTGGTAGACACG-BHQ1-3′

24

0.1

RNase P

RPP30

Forward

5′-GGCGGTGTTTGCAGATTT-3′

18

76

0.1

 

RPP30

Reverse

5′-AGCGGCTGTCTCCACAAG-3′

18

0.1

 

RPP30

Probe

5′-ROX-GGGTTCTGACCTGAAGGCTCTGC-BHQ2-3′

23

0.1