Species | Gene target | Primer/probe | Primer/probe sequence | Length (bp) | Product size(bp) | Concentration((µM) |
---|---|---|---|---|---|---|
HBV | S | Forward | 5′-TGTGTCTGCGGCGTTTTATC-3′ | 20 | 139 | 0.2 |
 | S | Reverse | 5′-CATGGTCCCGTGCTGGTAG-3′ | 19 | 0.2 | |
 | S | Probe | 5′-VIC-ATCCTGCTGCTATGCCTCATCTTCTTG-BHQ1-3′ | 27 | 0.2 | |
HCV | 5’ UTR | Forward | 5′-TAGCCGAGTAGTGTTGGGTC-3′ | 20 | 95 | 0.1 |
 | 5’ UTR | Reverse | 5′-TCATGGTGCACGGTCTACG-3′ | 19 | 0.1 | |
 | 5’ UTR | Probe | 5′-CY5-TACTGCCTGATAGGGTGCTTGCG-BHQ3-3′ | 23 | 0.1 | |
HEV | ORF2 | Forward | 5′-ATCTTGCTGACACGCTTCTCG-3′ | 21 | 158 | 0.4 |
 | ORF2 | Reverse | 5′-AGCTATACCCTTATCCTGCTGA-3′ | 22 | 0.4 | |
 | ORF2 | Probe | 5′-TAMRA-GTCTCAGCCAATGGCGAGCCGAC-BHQ2-3′ | 23 | 0.4 | |
T.pallidum | TPP47 | Forward | 5′-AGGTAAGCAGCATGGAGAGC-3′ | 20 | 117 | 0.1 |
 | TPP47 | Reverse | 5′-AGCCATCAGCCCTTTTCAGC-3′ | 20 | 0.1 | |
 | TPP47 | Probe | 5′-FAM-CCGCACGACCTTGTGGTAGACACG-BHQ1-3′ | 24 | 0.1 | |
RNase P | RPP30 | Forward | 5′-GGCGGTGTTTGCAGATTT-3′ | 18 | 76 | 0.1 |
 | RPP30 | Reverse | 5′-AGCGGCTGTCTCCACAAG-3′ | 18 | 0.1 | |
 | RPP30 | Probe | 5′-ROX-GGGTTCTGACCTGAAGGCTCTGC-BHQ2-3′ | 23 | 0.1 |