Table 3 The efficiency of ITS1 PCR- RFLP Diagnostic Method for Leishmania donovani species considering Microscopy as the gold standard at 95% confidence interval (CI)
Method | Gold-standard Microscope | Missed value | Total | PPV | NPV | Kappa value | P-value | |
|---|---|---|---|---|---|---|---|---|
Positive | Negative | |||||||
(= 47) | (= 43) | 0 | (= 90) | |||||
ITS1-PCR (LITSR/L5.8S) | ||||||||
 Positive | 42(87.5%) | 5 (11.9%) | 0 | 47 | 89.4% | 86.04% | 0.755 (0.60–0.80) | P < 0.05 |
 Negative | 6 (12.5%) | 37 (88.1%) | 0 | 43 | ||||