Fig. 3From: Detection of Leishmania donovani using ITS1-RFLP from positive and negative smear samples among clinically reported patients visiting University of Gondar Comprehensive Specialized HospitalA representative agarose gel electrophoresis (2.5%) of PCR- RFLP method using Hae III restriction enzyme. (L1) 50Â bp DNA ladder. (L2) 100Â bp DNA ladder. (PC) positive control for L.donovani (190, 80 and 50Â bp). Lane 1 to 10 was positive to L. donovani which provided (190, 80 and 50Â bp) bands, Lane 3 contained extra band which indicates mixed co- infectionBack to article page