Fig. 2

Flowchart depicting the process of molecular analyses. DNA deoxyribonucleic acid, rRNA ribosomal ribonucleic acid, PCR polymerase chain reaction; msp merozoite surface protein, PCR polymerase chain reaction. ^*Primary PCR using specific primers for Plasmodium spp. species. ^#Nested PCR using specific primers for P. falciparum, P. vivax and P. malariae, respectively ^&Nested PCR using specific primers for P. ovale curtisi and P. ovale wallikeri respectively, ^‡Nested PCR using specific primers for P. knowlesi.