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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Borrelia multiplex: a bead-based multiplex assay for the simultaneous detection of Borrelia specific IgG/IgM class antibodies

Fig. 4

Manual and automated processing of Borrelia multiplex. 662 serum samples were measured with the Borrelia multiplex and a common 2-step diagnostics. For the Borrelia multiplex processing was either manually or by pipetting robot. A, B Correlation between manual and automated processing of Borrelia multiplex. S/CO values were plotted against each other. A red dashed line shows the curve for identical S/CO values. Correlation analysis was performed according to Spearman. C–F Box-Whisker plots of S/CO values for Borrelia negative and positive samples. Samples were categorized according to the result of the 2-step diagnostics. Boxes include the median and the 25th and 75th percentiles. Whiskers are limited to 1.5 times IQR. Outliers are shown as depicted circles. A dashed line (S/CO = 1.0) indicates the threshold between positive and negative signal. The grey area below the line gives the borderline area (0.75 < S/Co < 1.0). For statistical analysis Mann–Whitney test (two-tailed) was used. P values were classified as follows: > 0.05 (ns), ≤ 0.05 (*), ≤ 0.01 (**), ≤ 0.001 (***)

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