Table 1 Primers and probes used for the qPCR assays to detect S. pneumoniae, H. influenzae, S. aureus, and K. pneumoniae
Organism (target gene) | Primer name | Nucleotide sequence of primers and probes |
|---|---|---|
S. pneumoniae (lytA) | lytA-CDC-f | 5ʹ-ACGCAATCTAGCAGATGAAGCA-3ʹ |
lytA-CDC-r | 5ʹ-TCGTGCGTTTTAATTCCAGCT-3ʹ | |
lytA-CDC-pr | 5ʹ-FAM-GCCGAAAACGCTTGATACAG GGAG-3ʹ- BHQ1 | |
S. aureus (nuc) | nucF | 5ʹ-GTTGCTTAGTGTTAACTTTAGTTGTA-3ʹ |
nucR | 5ʹ-AATGTCGCAGGTTCTTTATGTAATTT-3ʹ | |
nucPr | 5ʹ-FAM-AAGTCTAAGTAGCTCAGCAAATGCA-3ʹ- BHQ1 | |
K. pneumoniae (mdh) | mdhF | 5ʹ-CGGGCGTAGCGCGTAA-3ʹ |
mdhR | 5ʹ-GATACCCGCATTCACATTAAACAG-3ʹ | |
mdhP | 5ʹ-FAM-CCCGGCATGGATCGTTCCGA-3ʹ-BHQ1 | |
H. influenzae (hpd) | hpdF729 | 5ʹ-AGATTGGAAAGAAACACAAGAAAAAGA-3ʹ |
hpdR819 | 5ʹ-CACCATCGGCATATTTAACCACT-3ʹ | |
hpdPbr762i | 5ʹ-FAM-AAACATCCAATCGT-BHQ1-AATTATAGTTTACCCAATAACCC-3ʹ- C6 |