Table1 Overall analysis of the clinical samples via RT-qPCR, AuNPs-probe conjugate and PCR-based agglutination approach
Clinical samples | AuNPs-probe conjugate | PCR-based agglutination approach | Real-time PCR | |||
|---|---|---|---|---|---|---|
+ | − | + | − | + | − | |
Non-Leishmania skin wounds (n = 18) | 1 | 17 | 1 | 17 | 1 | 17 |
VL (n = 25) | 24 | 1 | 25 | – | 25 | – |
Blood samples from non-Endemic regions (n = 25) | – | 25 | – | 25 | – | 25 |
CL (n = 76) | 75 | 1 | 76 | – | 76 | – |
Specificity for VL | 100% | 100% | 100% | |||
Sensitivity for VL | 96% | 100% | 100% | |||
Specificity for CL | 94% | 94% | 94% | |||
Sensitivity for CL | 98% | 100% | 100% | |||