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Table 1 Specification details of the three serological tests used to detect Anti-SARS-CoV-2 antibodies

From: Test concordance and diagnostic accuracy of three serological assays for detection of anti-SARS-CoV-2 antibody: result from a population-based sero-epidemiological study in Delhi

Test specifications

WANTAI SARS-CoV-2 Ab ELISA¥

Quantitative anti-RBD ELISA (QRBD)

Chemiluminescence assay (CLIA)

Manufacturer

Beijing Wantai Biological Pharmacy Enterprise Co., Ltd, China

In-house kit developed by Translational Health Science And Technology Institute (THSTI), India

Abbott Laboratories Diagnostics, USA

Principle

ELISA

ELISA

Chemi-luminiscence Assay

Type

Qualitative

Quantitative

Qualitative

Target protein

Receptor binding domain of spike protein

Receptor binding domain of spike protein

Nucleocapsid protein of SARS-CoV-2

Antibody captured

Total antibody (IgM + IgG + IgA)

IgG

IgG

Test sensitivity

94.5%

100%

Unknown@

Test specificity

100%

100%

Unknown@

Reference Value

≥ 0.19 OD = Positive

< 0.19 OD = Negative

≤ 7.99 BAU#/ml = Negative

> 7.99—12.0 BAU#/ml = Equivocal

≥ 12.0 BAU#/ml = Positive

< 1.4 S/C$ = Negative

≥ 1.4 S/C$ = Positive

  1. ¥WANTAI Severe Acute Respiratory Syndrome Corona Virus-2 antibody Enzyme-Linked Immunosorbent Assay
  2. Quantitative anti-receptor binding domain Enzyme-Linked Immunosorbent Assay
  3. OD: optical density
  4. #BAU = ELISA Unit
  5. $S/C = Ratio of the mean chemiluminescent signal of sample and calibrator
  6. @As mentioned in the kit information brochure