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Table 1 Primer information used in BSI-HMGS

From: Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system

Target pathogen

Primer information

Concentration (μM/L)

Size (bp)

 

Sequence

Gram-negative bacteria

   

 K. pneumoniae

F: 5′-TGAGACTGTTCTGATTCCTGAT-3′

2

105

 

R: 5′-GTCGATTCGTTTCACCGGA-3′

2

 

 B. cepacia

F: 5′-TGTATCGGGCTTACCATGCGAT-3′

1

123

 

R: 5′-GTCTATACTCTGCACTGACTTCCT-3′

1

 

 P. mirabilis

F: 5′-TGCGTCGACCAATCGTTGTTAT-3′

1

140

 

R: 5′-GTAGTAGCTTTGAGACAGAAGGTGCTA-3′

1

 

 M. catarrhalis

F: 5′-TGACTGACTGCCACTGGTATGGATA-3′

1

150

 

R: 5′-GTATTTGGCACCACGCATAAT-3′

1

 

 S. marcescens

F: 5′-TGACCGGTAACACTGATCG-3′

1

170

 

R: 5′-GTGGTTTGCAGGTAAGGATC-3′

1

 

 H. influenzae

F: 5′-TGTCGTAACGCGTACTGTACCTG-3′

2

179

 

R: 5′-GTGATTGAGTTCTCGTTGCATCTT-3′

2

 

 P. aeruginosa

F: 5′-TGCAACCGGACCTGTGGCGC-3′

1

188

 

R: 5′-GCCTGGTAGTCTTCGGCACT-3′

1

 

 E. cloacae complex

F: 5′-TGCACGATGATGAAACCAAATGCGAC-3′

1

203

 

R: 5′-GTAGACCATTGTAGCATTCTGTTTCCGGT-3

1

 

 E. coli

F: 5′-TGCCTGGACGGATGAAAGTAGACA-3′

1

213

 

R: 5′-GTTCAGGAAGGAGCCGATATCATCTCTGA-3′

1

 

 A. baumannii

F: 5′-TGACTCTTTAATCCGCCGTGAA-3′

1

267

 

R: 5′-GTGCATCGTGGCTAATTTGTG-3′

1

 

 S. maltophilia

F: 5′-TGATAGGAACAGAAGGTCGAGATCAA-3′

2

277

 

R: 5′-GTCATTCGATCTGTGCCTTGTCGT-3′

2

 

 S. enteritidis

F: 5′-TGACGCGTCGTATTCGTTTACCAAAGC-3′

1

282

 

R: 5′-GTATTCATGCTTGTAGGCAATATCGG-3′

1

 

Gram-positive bacteria

   

 Staphylococcus

F: 5′-TCTCGTCAAATCGAATCWGC-3′

1

292

 

R: 5′-GTTCACCACCCAATTCYTCACGTT-3′

1

 

 S. aureus

F: 5′-TGTATTCGCAGGTCCTTCA-3′

2

302

 

R: 5′-GTTGACGAAACTGCGAGTGATTAAG-3′

2

 

 Streptococcus

F: 5′-TGACGTATTCCGTTCTAATACAGG-3′

1

220

 

R: 5′-GTACCGTCCGTGATAKAGATATCCA-3′

1

 

 S. pyogenes

F: 5′-TGGAAGGCGAGTTAATCAGGTAG-3′

1.5

248

 

R: 5′-GTAGAAGCCTGAGGAATCGCTA-3′

1.5

 

 S. agalactiae

F: 5′-TGCTGGGTTAGGAGTAGGTTTGTCAGC-3′

2

259

 

R: 5′-GTTGATTGCGTGTACCTTGCGATAATG-3′

2

 

 S. pneumoniae

F: 5′-TGCTATACAATGGACGACCCTTTC-3′

2

345

 

R: 5′-GTAGTTTGTCTGCTTCGACCTTTAT-3′

2

 

 E. faecalis

F: 5′-TGTCAAGACCAGTGTTCACGAA-3′

2

117

 

R: 5′-GTGTCATGAAACGATGTTTGG-3′

2

 

 E. faecium

F: 5′-TGTAATCAGGAGTCGTTCTTGCGAT-3′

1.5

353

 

R: 5′-GTGATATGGAAGTTTGTGCCGGTCATAT-3′

1.5

 

Fungus

   

 C. albicans

F: 5′-TGTTGTTTGCCTTATTGGTTGCCT-3′

2

128

 

R: 5′-GTCTTTGGATAACCGTTGATGGTAC-3′

2

 

 C. tropicalis

F: 5′-TGACTCTAGAAAGTCGCGTATTTC-3′

1

188

 

R: 5′-GTCTGTGATTGAGAATGATCGCT-3′

1

 

 C. parapsilosis

F: 5′-TGCTGTTTGGGCGTCGTTG-3′

2

231

 

R: 5′-GTCAAGGATAAGACGCGTATCTCCCTTC-3′

2

 

 C. glabrata

F: 5′-TGGTTGCACGATATACAGGGACAC-3′

1

332

 

R: 5′-GTGCACACGCTGTATATGTTCTTGTT-3′

1

 

IC

   

 IC

F: 5′-TGAACGTCTTACACCTCCTAAACA-3′

2

367

 

R: 5′--GTCACGTTCCGGCATTGTCTTAT-3′-

2

 
  1. IC: internal control; F: forward; R: reverse