Fig. 3

BSI-HMGS can simultaneously detect 24 pathogens in a single reaction. A mixture of all 25 plasmids, each at a concentration of 100 copies/μL, was detected by BSI-HMGS, and all specific peaks were found to be above the positive cut-off fluorescence signal of 500 rfu. No non-specific peak was observed, indicating that the optimized BSI-HMGS assay has a high level of sensitivity for simultaneously detecting the 24 target pathogen genes and the internal control gene in a single reaction. HMGS: high-throughput multiplex genetic detection system; IC: internal control