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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system

Fig. 1

PCR inhibitors are present in various blood culture bottles. To explore the presence of PCR inhibitors in the matrix components of predominantly used blood culture bottles, mixtures of P. mirabilis suspension with matrix components from each bottle were extracted, amplified, and detected (A–F). To verify the counter inhibition efficacy of the pre-treatment protocol, the pre-treated mixture of P. mirabilis suspension and matrix components from Versa TREK Redox was examined (G). The mixture of P. mirabilis suspension and ddH2O was also examined as a positive control (H). The mixtures containing matrix components from most blood culture bottles showed no amplified peaks. Notably, no distinct non-specific primer dimer peaks were observed from 0 to 100 nt in these tests, which should have appeared in every successful amplification. In contrast, the pre-treated mixture and control mixture showed specific p. mirabilis peaks along with normal primer dimer peaks. Likewise, the mixture containing matrix component from the Versa TREK Redox anaerobic bottle showed correct peaks, which may result from its unique composition compared with other bottles. The dashed red lines denote the demarcation between primer dimer peaks (0–100 nt) and peaks of target pathogens (100–400 nt)

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